A Microfluidic Hydrogel Capable of Cell Preservation without Perfusion Culture under Cell‐Based Assay Conditions

Xu, Yan; Sato, Kae; Mawatari, Kazuma; Konno, Tomohiro; Jang, Kyung‐In; Ishihara, Kazuhíko; Kitamori, Takehiko

doi:10.1002/adma.201000006

Cited by 50 publications

(40 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A chondrocyte marker, Col2a1, was highly expressed from the adhered cells onto PMBV352 with BMP2 signal rather than conventional materials. This result indicates that PMBV352 is a useful artificial stem cell niche to guide the desired differentiation property [114][115][116].…”

Section: Cell-function Controllable Surfacesmentioning

confidence: 78%

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

Iwasaki

Ishihara

2012

Science and Technology of Advanced Materials

Self Cite

261

216

View full text Add to dashboard Cite

This review article describes fundamental aspects of cell membrane-inspired phospholipid polymers and their usefulness in the development of medical devices. Since the early 1990s, polymers composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units have been considered in the preparation of biomaterials. MPC polymers can provide an artificial cell membrane structure at the surface and serve as excellent biointerfaces between artificial and biological systems. They have also been applied in the surface modification of some medical devices including long-term implantable artificial organs. An MPC polymer biointerface can suppress unfavorable biological reactions such as protein adsorption and cell adhesion -in other words, specific biomolecules immobilized on an MPC polymer surface retain their original functions. MPC polymers are also being increasingly used for creating biointerfaces with artificial cell membrane structures.

show abstract

Section: Cell-function Controllable Surfacesmentioning

confidence: 78%

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

Iwasaki

Ishihara

2012

Science and Technology of Advanced Materials

Self Cite

261

216

View full text Add to dashboard Cite

show abstract

“…We have previously reported effective surface modification methods to achieve cell micropatterning directly inside the microchannel via photochemical reaction. 23 This technique is based on the combination of a nonbiofouling polymer, 2-methacryloyloxyethyl phosphorylcholine ͑MPC͒, 24,25 and a nitrobenzyl photocleavable linker ͑PL͒. 26,27 The MPC polymer, PL, and silanization reagents are covalently bonded to the glass surface; the MPC polymer is selectively removed by UV irradiation, forming a cell-adherent hydrophobic surface.…”

Section: Introductionmentioning

confidence: 99%

Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system

Jang

Tanaka

et al. 2010

Biomicrofluidics

Self Cite

View full text Add to dashboard Cite

Recently, interest in single cell analysis has increased because of its potential for improving our understanding of cellular processes. Single cell operation and attachment is indispensable to realize this task. In this paper, we employed a simple and direct method for single-cell attachment and culture in a closed microchannel. The microchannel surface was modified by applying a nonbiofouling polymer, 2-methacryloyloxyethyl phosphorylcholine ͑MPC͒ polymer, and a nitrobenzyl photocleavable linker. Using ultraviolet ͑UV͒ light irradiation, the MPC polymer was selectively removed by a photochemical reaction that adjusted the cell adherence inside the microchannel. To obtain the desired single endothelial cell patterning in the microchannel, cell-adhesive regions were controlled by use of round photomasks with diameters of 10, 20, 30, or 50 m. Single-cell adherence patterns were formed after 12 h of incubation, only when 20 and 30 m photomasks were used, and the proportions of adherent and nonadherent cells among the entire UVilluminated areas were 21.3% Ϯ 0.3% and 7.9% Ϯ 0.3%, respectively. The frequency of single-cell adherence in the case of the 20 m photomask was 2.7 times greater than that in the case of the 30 m photomask. We found that the 20 m photomask was optimal for the formation of single-cell adherence patterns in the microchannel. This technique can be a powerful tool for analyzing environmental factors like cell-surface and cell-extracellular matrix contact.

show abstract

“…This mild crosslinking reaction occurs in physiological environment (cell culture medium, 37°C and neutral pH) and is therefore suitable for the encapsulation of biological agents. Previous research has proved that this hydrogel enabled the safe encapsulation of bacteria [42], living cells [43][44][45], and bioactive molecules [46,47]. The PMBV/PVA hydrogel has also been used in vivo to prevent tissue adhesion surrounding tendon [48].…”

Section: Spontaneously Forming Hydrogels Designed With Phospholipid Pmentioning

confidence: 97%

Cytocompatible and spontaneously forming phospholipid polymer hydrogels

Gao

Konno

Ishihara

2015

European Polymer Journal

Self Cite

View full text Add to dashboard Cite

A Microfluidic Hydrogel Capable of Cell Preservation without Perfusion Culture under Cell‐Based Assay Conditions

Cited by 50 publications

References 28 publications

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system

Cytocompatible and spontaneously forming phospholipid polymer hydrogels

Contact Info

Product

Resources

About