A microfluidic streaming potential analyzer for label-free DNA detection

Li, Yuefang; Lai, Sze Nga; Zheng, Bo

doi:10.1016/j.snb.2017.12.130

Cited by 17 publications

(10 citation statements)

References 38 publications

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“…e.g. 10 nM (Li et al, 2018) to 460 fM (Cavallaro et al, 2019), is a clear testament of this behaviour. Aiming to understand such a large variation in the sensitivity, we utilized here a recently developed theoretical model to simulate the sensor response as a function of the surface coverage for three different classes of proteins in terms of their size and charges (Fig.…”

Section: Discussionmentioning

confidence: 91%

“…In the context of electrokinetic sensors (Dev et al, 2016), where an adsorbed particle perturbs both the local hydrodynamics and electrostatics (Joly et al, 2004), the influence of physical properties like the size and charge of the adsorbed particle is a particularly impor tant consideration. The electrokinetic principles such as streaming current and streaming potential have been widely explored for precise determination of surface coverage and deposition kinetics of various organic/inorganic particles (Downs et al, 2019;Hanaor et al, 2014) as well as biological molecules/particles such as antibodies (Dev et al, 2016), DNA (Li et al, 2018) and extracellular vesicles (Cavallaro et al, 2019). The streaming current ( ), or the streaming potential ( ), is generated when an electrolyte is forced to flow through a channel under an applied pressure.…”

Section: Introductionmentioning

confidence: 99%

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Influence of molecular size and zeta potential in electrokinetic biosensing

Sahu

Stiller

Cavallaro

et al. 2020

Biosensors and Bioelectronics

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Electrokinetic principles such as streaming current and streaming potential are extensively used for surface characterization. Recently, they have also been used in biosensors, resulting in enhanced sensitivity and simpler device architecture. Theoretical models regarding streaming current/potential studies of particle-covered surfaces have identified features such as the particle size, shape and surface charge to influence the electrokinetic signals and consequently, the sensitivity and effective operational regime of the biosensor. By using a set of well-characterized proteins with varying size and net surface charge, this article experimentally verifies the theoretical predictions about their influence on the sensor signal. Increasing protein size was shown to enhance the signal when their net surface charge was either opposite to that of the sensor surface, or close to zero, in agreement with the theoretical predictions. However, the effect gradually saturates as the protein size exceeds the coulomb screening length of the electrolyte. In contrast, the proteins containing the same type of charge as the surface show little or no difference, except that the signal inverts. The magnitude of the surface charge was also shown to influence the signal. The sensitivity of the technique for protein detection varied over two orders of magnitude, depending upon the size and surface charge. Furthermore, the capacity of the electrokinetic method for direct electrical detection of various proteins, including those carrying little or no net electric charges, is demonstrated.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Influence of molecular size and zeta potential in electrokinetic biosensing

Sahu

Stiller

Cavallaro

et al. 2020

Biosensors and Bioelectronics

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show abstract

“…Hence, DA concentration in human body is usually used to identify several neural diseases such as memory loss, drug addiction, psychiatric problems, Parkinson's disease etc. [103,159]. In view of this, lot of work has been done on the detection of DA.…”

Section: Dopaminementioning

confidence: 99%

Optical Biomedical Diagnostics Using Lab-on-Fiber Technology: A Review

2022

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Point-of-care and in-vivo bio-diagnostic tools are the current need for the present critical scenarios in the healthcare industry. The past few decades have seen a surge in research activities related to solving the challenges associated with precise on-site bio-sensing. Cutting-edge fiber optic technology enables the interaction of light with functionalized fiber surfaces at remote locations to develop a novel, miniaturized and cost-effective lab on fiber technology for bio-sensing applications. The recent remarkable developments in the field of nanotechnology provide innumerable functionalization methodologies to develop selective bio-recognition elements for label free biosensors. These exceptional methods may be easily integrated with fiber surfaces to provide highly selective light-matter interaction depending on various transduction mechanisms. In the present review, an overview of optical fiber-based biosensors has been provided with focus on physical principles used, along with the functionalization protocols for the detection of various biological analytes to diagnose the disease. The design and performance of these biosensors in terms of operating range, selectivity, response time and limit of detection have been discussed. In the concluding remarks, the challenges associated with these biosensors and the improvement required to develop handheld devices to enable direct target detection have been highlighted.

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“…The bottom image of Figure 5a shows surface treatments, e.g., etching and oxidation, had a profound influence on the zeta potential of the silicon nitride surface [109], where the oxidized nitride surface might resist bacterial colonization due to its extreme negative surface charge. Li et al [3] designed a DNA detection system based on DNA-PNA hybridization inside a microchannel, measured with a streaming potential analyzer, and obtained a detection limit of 10 nM. Yu et al [114] developed a self-powered urea sensor by immobilizing catalytic enzymes on the microfluidic channel.…”

Section: Streaming Potential Measurementmentioning

confidence: 99%

Surface Potential/Charge Sensing Techniques and Applications

Chen

Dong

Yang

2020

Sensors

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Surface potential and surface charge sensing techniques have attracted a wide range of research interest in recent decades. With the development and optimization of detection technologies, especially nanosensors, new mechanisms and techniques are emerging. This review discusses various surface potential sensing techniques, including Kelvin probe force microscopy and chemical field-effect transistor sensors for surface potential sensing, nanopore sensors for surface charge sensing, zeta potentiometer and optical detection technologies for zeta potential detection, for applications in material property, metal ion and molecule studies. The mechanisms and optimization methods for each method are discussed and summarized, with the aim of providing a comprehensive overview of different techniques and experimental guidance for applications in surface potential-based detection.

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A microfluidic streaming potential analyzer for label-free DNA detection

Cited by 17 publications

References 38 publications

Influence of molecular size and zeta potential in electrokinetic biosensing

Influence of molecular size and zeta potential in electrokinetic biosensing

Optical Biomedical Diagnostics Using Lab-on-Fiber Technology: A Review

Surface Potential/Charge Sensing Techniques and Applications

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