A microsatellite panel for mating system analysis and broodstock management of captive long-snouted seahorse Hippocampus guttulatus

López, Almudena; Pardo, Belén G.; Planas, Miquel; Quintas, Patricia; Martı́nez, Paulino; Bouza, Carmen

doi:10.1016/j.aquaculture.2012.05.021

Cited by 6 publications

(18 citation statements)

References 42 publications

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“…A first set of six loci useful for parentage analysis in H . guttulatus was amplified in a single PCR for all wild seahorses and offspring ( Hgu -USC5, Hgu -USC6, Hgu -USC7, Hgu -USC8, Hgut 4, Hgut 6) following López et al [ 35 ]. A second PCR multiplex with the remaining seven loci was amplified for wild seahorses in a volume of 10 μL with 1X Qiagen Multiplex PCR Master Mix, 50 ng of DNA template, and 0.08, 0.10, 0.10, 0.08, 0.15, 0.15, 0.10 μM of each primer for Hgu -USC2, Hgu -USC4, Hgu -USC9, Hgu -USC12, Hgu -USC13, Hca μ25, Hca μ36, respectively, using NED, 6-FAM, VIC, VIC, 6-FAM, PET, PET as 5’ fluorescent label for their respective forward primer.…”

Section: Methodsmentioning

confidence: 99%

“…Microsatellite markers have proven to be useful to ascertain the mating system and to investigate parentage and kinship of wild and captive populations of some seahorse species [ 31 – 34 ], including H . guttulatus [ 30 , 35 ]. Genetic analysis of different populations of this latter species revealed single maternity per brood [ 30 , 35 ], and serial monogamy in captivity [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…guttulatus [ 30 , 35 ]. Genetic analysis of different populations of this latter species revealed single maternity per brood [ 30 , 35 ], and serial monogamy in captivity [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

et al. 2015

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This study was focused on conservation genetics of threatened Hippocampus guttulatus on the Atlantic coast of NW Iberian Peninsula. Information about spatial structure and temporal stability of wild populations was obtained based on microsatellite markers, and used for monitoring a captive breeding program firstly initiated in this zone at the facilities of the Institute of Marine Research (Vigo, Spain). No significant major genetic structure was observed regarding the biogeographical barrier of Cape Finisterre. However, two management units under continuous gene flow are proposed based on the allelic differentiation between South-Atlantic and Cantabrian subpopulations, with small to moderate contemporary effective size based on single-sample methods. Temporal stability was observed in South-Atlantic population samples of H. guttulatus for the six-year period studied, suggesting large enough effective population size to buffer the effects of genetic drift within the time frame of three generations. Genetic analysis of wild breeders and offspring in captivity since 2009 allowed us to monitor the breeding program founded in 2006 in NW Spain for this species. Similar genetic diversity in the renewed and founder broodstock, regarding the wild population of origin, supports suitable renewal and rearing processes to maintain genetic variation in captivity. Genetic parentage proved single-brood monogamy in the wild and in captivity, but flexible short- and long-term mating system under captive conditions, from strict monogamy to polygamy within and/or among breeding seasons. Family analysis showed high reproductive success in captivity under genetic management assisted by molecular relatedness estimates to avoid inbreeding. This study provides genetic information about H. guttulatus in the wild and captivity within an uncovered geographical range for this data deficient species, to be taken into account for management and conservation purposes.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

et al. 2015

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“…In this study, the mean number of alleles was smaller than that re ported for other fishes (Munkres et al 2007, Li et al 2010, López et al 2012, Luo et al 2014a. As a consequence, in our simulations, at least 6 microsatellite markers were needed to achieve 95% assignment success when there was information available regarding the parents.…”

Section: Discussionmentioning

confidence: 87%

“…This accounted for an average of 8.33% at the 3 loci, a value higher than that reported for most other species of fish (Hoffman & Amos 2005, Castro et al 2007, Luo et al 2014a). Thus, when choosing markers for parentage assignment, besides polymorphism and null allele frequency, the quality of the allelic pattern should also be taken into consideration since loci with a high number of stutter bands (or shadow bands) are often associated with high typing error rates (Estoup et al 1998, López et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)

Shao¹,

Luo²,

Wei³

et al. 2017

Aquat. Biol.

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We evaluated the use of microsatellite markers to identify parentage in Brachy mystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellitebased parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species.

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Embryonic development and microsatellite-based parentage assignment of seaweed pipefish Syngnathus schlegeli

et al. 2019

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A microsatellite panel for mating system analysis and broodstock management of captive long-snouted seahorse Hippocampus guttulatus

Cited by 6 publications

References 42 publications

Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

Conservation Genetics of Threatened Hippocampus guttulatus in Vulnerable Habitats in NW Spain: Temporal and Spatial Stability of Wild Populations with Flexible Polygamous Mating System in Captivity

Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)

Embryonic development and microsatellite-based parentage assignment of seaweed pipefish Syngnathus schlegeli

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