A microtiter plate procedure for evaluating fungal functional diversity

A bacterial triple-species consortium that synergistically metabolizes the phenylurea herbicide linuron was studied to determine whether synergy is extended toward the metabolism of other C-sources. The metabolic performance and range of the individual consortium members were compared with those of paired and three-species combinations in Biolog GN2 MicroPlate assays. The strain combinations showed an increase in the rate and extent of utilization of 80% of the C-sources that were utilized by either one or more of the individual consortium members and the additional utilization of eight C-sources for which oxidation was not observed for the individual strains. When one of the three strains was replaced by bacterial strains 'foreign' to the consortium, either belonging to the same genus or to other genera, mainly antagonistic effects occurred. The data suggest that the consortium members cooperate in the metabolism of C-sources in addition to linuron. This feature can contribute in consolidating consortium composition when linuron is absent or present at low concentrations.

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“… Total numbers are given as well as numbers per indicated substrate group as proposed by Dobranic & Zak (). …”

Section: Resultsmentioning

confidence: 99%

Carbon source utilization profiles suggest additional metabolic interactions in a synergistic linuron-degrading bacterial consortium

Horemans

Smolders

Springael

2012

FEMS Microbiol Ecol

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“…Substrate utilization profiles from BIOLOG Phenotype MicroArrays and API 50 CH biochemical tests and enzyme profiles from API ZYM have been applied previously to assess functional diversity and nutritional profiles of individual fungal species (Dobranic & Zak, 1999; Tanzer et al , 2003; Druzhinina et al , 2006) as well as in the analysis of fungal communities (Buyer et al , 2001) and microbial bioprocesses (Tiquia, 2002). In our study, we investigated patterns of carbon source utilization by two marine species of the fungus Dendryphiella , D. arenaria and D. salina , to detect traits differentiating the two species and to learn to what extent they have the ability to use various carbon sources.…”

Section: Discussionmentioning

confidence: 99%

Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina

Cruz¹,

Schulz²,

Kubicek³

et al. 2006

FEMS Microbiology Ecology

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Carbon utilization by the marine Dendryphiella species, D. arenaria and D. salina, was investigated to detect differences in utilization and traits associated with their adaptation to the marine habitat. Fifty-four strains were isolated world-wide and tested for the utilization of various carbon sources using BIOLOG phenotype MicroArray (PM) and for the production of extracellular enzymes on solid culture media and on API ZYM assay strips. PM analysis showed that the fastest growth occurred on several monosaccharides and amino acids, 2-keto-d-gluconic acid, succinamide and turanose. Some polyols were poor carbon sources. However, the two species differed in their utilization rates of carbon sources, forming three major clusters: two separate clusters for D. arenaria and D. salina and a third cluster in which strains of the two species formed separate subclades that correlated with geographic origin. Several carbon sources were also found useful in differentiating the two speices. Dendryphiella salina did not utilize xylitol and quinic acid, whereas D. arenaria grew well on these substrates. The latter failed to grow on sorbitol and grew slowly on mannitol, both were good substrates for the former. There were also no qualitative differences between the extracellular enzymes produced, although laccase and peroxidase activities were confined only to some strains. The physiological similarities exhibited by the two species support the close relationship between D. arenaria and D. salina.

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“…Bacterial inoculations were accomplished by transferring 150 μl of solution to each of 96 wells in each EcoPlate and fungal inoculations using only 100 μl per well (Buyer et al 2001;Donbranic and Zak 1999). All plates were placed in polyethylene bags to reduce desiccation while incubating.…”

Section: Microbial Propertiesmentioning

confidence: 99%

Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China

et al. 2008

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Nitrogen (N) addition has been well documented to decrease plant biodiversity across various terrestrial ecosystems. However, such generalizations about the impacts of N addition on soil microbial communities are lacking. This study was conducted to examine the impacts of N addition (urea-N fertilizer) on soil microbial communities in a semi-arid temperate steppe in northern China. Soil microbial biomass carbon (C), biomass N (MBN), net N mineralization and nitrification, and bacterial and fungal community level physiological profiles (CLPP) along an N addition gradient (0-64 g N m −2 year −1 ) were measured. Three years of N addition caused gradual or step increases in soil NH 4 -N, NO 3 -N, net N mineralization and nitrification in the early growing season. The reductions in microbial biomass under high N addition levels (32 and 64 g N m −2 year −1 ) are partly attributed to the deleterious effects of soil pH. An N optimum between 16 and 32 g N m −2 year −1 in microbial biomass and functional diversity exists in the temperate steppe in northern China. Similar N loading thresholds may also occur in other ecosystems, which help to interpret the contrasting observations of microbial responses to N addition.

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A microtiter plate procedure for evaluating fungal functional diversity

Cited by 49 publications

References 25 publications

Carbon source utilization profiles suggest additional metabolic interactions in a synergistic linuron-degrading bacterial consortium

Carbon source utilization profiles suggest additional metabolic interactions in a synergistic linuron-degrading bacterial consortium

Carbon source utilization by the marine Dendryphiella species D. arenaria and D. salina

Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China

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