A mini-pig model for evaluating the efficacy of autologous platelet patches on induced acute full thickness wound healing

Abstract

Background Autologous platelet concentrates are currently widely used across different areas of regenerative medicine in order to enhance the wound healing process. Although several protocols for platelet concentrates are available, their application remains difficult due to different protocols leading to distinct products with vary potential biological uses. In this study, we attempted to make a platelet patch (PP) using mixtures of platelet rich plasma (PRP) injection and platelet rich fibrin (PRF) to promote wound repair and regeneration. Results Experiments were performed using a full-thickness wound model in mini-pigs. Autologous PRP, PRF and PP were prepared immediately before creating four full-thickness skin wounds in pigs. We quantified concentrations of platelets, thrombin and various growth factors to ensure that the desired effect can be produced. After surgery, hydrocolloid dressing, PRP injection, PRF and PP was applied to experimentally induced wounds. Application efficacy was evaluated by measurement of wound sizes and histological examination. The results indicated that all wounds showed a significant size reduction. Wound repair efficacy in response to PP treatment exhibited enhanced re-epithelialization compared to PRP and PRF ( P < 0.05) and higher wound contraction than did PRF application ( P < 0.05). Another aspect, experiment using DsRed transgenic pigs as blood donors demonstrated that leucocytes in PP were incorporated into the wound bed at the end of the study, suggesting that leucocytes activity is stimulated in response to PP application. Safety of the experimental processes was also confirmed by examination of organ biopsies. Conclusions We used a mini-pig model to evaluate the efficacy of lab-made PP on induced full-thickness wound healing. Results demonstrated that application of one piece of PP was enough to obtain comparable efficacy versus general utilization of PRP or PRF for wound care. We also demonstrated that leucocytes in PP were incorporated into the wound bed and no safety concerns have been found in the whole experiment. This study provides a novel and feasible method for veterinary or clinical wound care.

Clinical, Molecular and Histopathological Evaluation of the Effect Autologus PRP &amp; PRF on the Second Intension Wound Healing in Dogs

“…Wound healing of the member of PRF group showed no signi cant difference from PRP treated animals but still lower in the healing percentage rate. Our results come in agreement with previous studies [36]. These reported that the liquid nature of the PRP increases the power of diffusion and invasion to the tissue, so the effect is faster than PRF which has a gel in nature.…”

Autologous Platelet-Rich Plasma Enhances the Healing of Large Cutaneous Wounds in Dogs

“…PRP is a readily available and cost-effective therapy rich in growth factors and cytokines that enhances and supports tissue regeneration by stimulating cell migration and proliferation. Its application for the treatment of skin ulcers has already been widely reported both in humans (9,(22)(23)(24)(25) and animals (1,16,29,37,39,42). However, studies regarding the clinical efficacy of PRP in dogs for skin wound healing are rare.…”

“…Positive results have been obtained also in the veterinary field, both in acute wound experimental models (26) or in the clinical practice to treat chronic non-healing wounds. In the literature, there are various reports on the use of PRP in veterinary medicine demonstrating that it might accelerate and stimulate the healing of acute wounds and promote the repair of impaired chronic wounds as observed in dogs (12,(27)(28)(29)(30)(31)(32), horses (1,16,(33)(34)(35)(36), cats (37,38), sheep (39), goat (40), and pigs (41,42). However, sometimes these results appear to be discordant, i.e., no positive effects of PRP are shown (2,43,44).…”

Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen

“…Applications of PRP in fracture repair had been shown in both dogs [49] and rabbit [48] by promoting bone healing. Interestingly, injection of PRP also promoted wound healing of soft tissue defects [50,51] as well as periodontal regeneration [52]. Since the present study did not evaluate the alteration of growth factors in PRP, it remains unknown whether the use of carprofen as a separation layer affecting the function of platelet-derived growth factors in PRP products.…”