A miniaturized quantitative polymerase chain reaction system for DNA amplification and detection

Wang, Jung-Hao; Chien, Liang-Ju; Hsieh, Tar‐Hwa; Luo, Ching‐Hsing; Chou, Wen-Pin; Chen, Ping‐Hei; Chen, Pei‐Jer; Lee, Dasheng; Lee, Gwo‐Bin

doi:10.1016/j.snb.2009.06.034

Cited by 48 publications

(29 citation statements)

References 35 publications

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“…In thermal microfluidics systems, different heat sources such as preheated liquids, 19,20 Joule heating, [21][22][23][24][25] microwave heating, [26][27][28] and chemical reactions 29 have been used. Resistive heating (Joule heating) is selected as a heat source in this work to achieve homogeneous heating with a wide operational temperature range, better heat control compared to other methods 30 and for ease of integration onto thin film substrates.…”

Section: Design and Fabrication Of The Thermal Componentsmentioning

confidence: 99%

A novel on-chip three-dimensional micromachined calorimeter with fully enclosed and suspended thin-film chamber for thermal characterization of liquid samples

et al. 2014

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A microfabricated calorimeter (l-calorimeter) with an enclosed reaction chamber is presented. The 3D micromachined reaction chamber is capable of analyzing liquid samples with volume of 200 nl. The thin film low-stress silicon nitride membrane is used to reduce thermal mass of the calorimeter and increase the sensitivity of system. The l-calorimeter has been designed to perform DC and AC calorimetry, thermal wave analysis, and differential scanning calorimetry. The l-calorimeter fabricated with an integrated heater and a temperature sensor on opposite sides of the reaction chamber allows to perform thermal diffusivity and specific heat measurements on liquid samples with same device. Measurement results for diffusivity and heat capacitance using time delay method and thermal wave analysis are presented. V C 2014 AIP Publishing LLC. [http://dx

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Section: Design and Fabrication Of The Thermal Componentsmentioning

confidence: 99%

A novel on-chip three-dimensional micromachined calorimeter with fully enclosed and suspended thin-film chamber for thermal characterization of liquid samples

et al. 2014

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“…Nucleic acid analysis generally consists of three major steps: DNA isolation, amplification, and detection. However most researchers have reported LOC systems in which only one or more parts of the nucleic acid analysis steps are integrated 1, [3][4][5][6][7][8][9] . For the commercialization of such bio-chip analysis system, the system management functions such as the data processing and the user interface are also required as well as biochemical processes 1,4-10 .…”

Section: Introductionmentioning

confidence: 99%

Efficient control system for PCR chips

Kim

Kim²,

Lee³

2011

SPIE Proceedings

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Effective host-local system architecture for PCR chips is presented. The functions required for the control of the chips and their partitioning into the host and the local system are qualitatively analyzed. The results indicated that, most functions can be resided in the host to deliver the benefits on the development period, the system cost, and the maintenance. The control system was implemented with PC as the host and the local system was connected by USB interface, obtaining the superior development and the GUI design environment. A PCB-based PCR chip example was constructed and tested to verify the proposed system.

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“…However, the numbers of thermal cycles are usually not adjustable, and increasing the cycle numbers results in larger device size. Utilization of circular channel loop with circularly arranged heaters was also proposed [14,15]. PCR mixture circulates in the channel loop with three specific temperature zones.…”

Section: Introductionmentioning

confidence: 99%

“…1(c)). Note that since the actuators can be easily driven with DC voltages, the proposed approach eliminates the need of bulky equipments for driving fluid, such as syringe pumps/injectors [15,19], special power supplies [17], or air compressors [16,21]. Figures 3(b) and 3(c) are the schematic illustration of the combined configuration viewed along the xdirection indicated in Fig.…”

Section: Introductionmentioning

confidence: 99%

A Self-Contained Portable Polymerase-Chain-Reaction System Integrated with Electromagnetic Mini-Actuators for Bi-Directional Fluid Transport

Chia¹,

Yang²,

Cheng³

et al. 2011

J. mech.

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In this paper, the development of a portable polymerase chain reaction (PCR) device is presented. Integrating electromagnetic mini-actuators for bi-directional fluid transport, the proposed device, whose dimension is 67mm  66mm  25mm, can be fully operated with a 5V DC voltage. The device consists of four major parts: A disposable channel chip in which PCR mixture is manipulated and reacted, a heater chip which generates different temperature zones for PCR reaction, a linear actuator array for pumping PCR mixture, and a circuit module for controlling and driving the system. The advantages of the device include the rapid temperature responses associated with continuous-flow-type PCR devices, as well as the programmable thermal cycling associated with chamber-type PCR devices. The thermal characteristics are measured and discussed. PCR amplification is successfully performed for the 122 bp segment of MCF-7/adr cell line. Due to its small footprint, this self-contained system potentially can be employed for point-of-care (POC) applications.

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A miniaturized quantitative polymerase chain reaction system for DNA amplification and detection

Cited by 48 publications

References 35 publications

A novel on-chip three-dimensional micromachined calorimeter with fully enclosed and suspended thin-film chamber for thermal characterization of liquid samples

A novel on-chip three-dimensional micromachined calorimeter with fully enclosed and suspended thin-film chamber for thermal characterization of liquid samples

Efficient control system for PCR chips

A Self-Contained Portable Polymerase-Chain-Reaction System Integrated with Electromagnetic Mini-Actuators for Bi-Directional Fluid Transport

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