A mitochondria-targeted near-infrared fluorescent probe for imaging viscosity in living cells and a diabetic mice model

Abstract

A mitochondria-targeted near-infrared fluorescent probe NIR-V with 700 nm emission was designed to monitor cell viscosity changes, which was applied to detect the intracellular viscosity and imagine pancreatic tissue in diabetic mouse model.

Semiconducting polymer dots as fluorescent probes forin vitrobiosensing

“…This journal is © The Royal Society of Chemistry 2022 of diseases. [98][99][100][101][102][103] Accurate quantification of specific biomolecules in biological samples in vitro can help us precisely diagnose diseases and guide disease treatment, which is of great clinical value. Meanwhile, the onset and progression of some diseases may involve multiple biomolecules, and thus the multiplexed detection of targets in complex sample media can provide more valuable information demanding less time and cost.…”

Recent Progress in Fluorescent Probes for Diabetes Visualization and Drug Therapy

“…Probe 6 is regarded as the first probe to estimate the viscosity behavior between blood samples of diabetic and hypertensive patients (Figure 4B). Zhou's group created a mitochondria-targeted NIR viscosity fluorescent probe (7) [61], consisting of 2,3,3-trimethyl-3H-indolenine and 4-(dimethylamino) cinnamaldehyde (Figure 5A). Probe 7 had a double twistable ethylene structure with twisted intramolecular charge transfer (TICT) capacity.…”

“…Figure 5B shows that the viscosity of the insulin group was lower than that of the diabetic group, which indicates that the probe is suitable for monitoring viscosity behavior and imaging pancreatic tissue in diabetic mice. Zhou's group created a mitochondria-targeted NIR viscosity fluorescent probe (7) [61], consisting of 2,3,3-trimethyl-3H-indolenine and 4-(dimethylamino) cinnamaldehyde (Figure 5A). Probe 7 had a double twistable ethylene structure with twisted intramolecular charge transfer (TICT) capacity.…”

A novel mitochondrion-targeted fluorescent probe for detecting viscosity in living cells and zebrafishes

“…Therefore, they could be adapted to monitor viscosity changes at microscopic scales in real time. [20][21][22][23][24][25][26][27][28][29][30][31] The application of fluorescent probes for monitoring intracellular viscosity changes, particularly at the subcellular level, has become a hot research spot. To date, several mitochondrion-targeted fluorescent probes have been reported.…”