A Modification of the Formol-Ether Concentration Technique for Increased Sensitivity in Detecting Schistosoma Mansoni Eggs

Knight, Wilda B.; Hiatt, Robert A.; Cline, Barnett L.; Ritchie, L. S.

doi:10.4269/ajtmh.1976.25.818

Cited by 116 publications

(62 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cysts were concentrated from stool samples using formalin-ether concentration method (Knight et al, 1976). DNA from the concentrated cysts was isolated using QIAamp DNA stool kit as per protocol provided by the supplier.…”

Section: Isolation Of Dna From Axenic Cultures and Stool Samplesmentioning

confidence: 99%

Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar

Srivastava¹,

Bhattacharya²,

Paul³

2005

Experimental Parasitology

View full text Add to dashboard Cite

Entamoeba histolytica and Entamoeba dispar are two morphologically indistinguishable species that are found in the human gut. Of the two, E. histolytica is considered to be pathogenic while E. dispar is nonpathogenic. To generate molecular probes to detect and distinguish between the two species, we utilized repeat sequences present in Entamoeba genome. We have developed probes and primers from rDNA episomes, and unidentified Entamoeba EST1 repeat for this purpose, and used them for dot blot hybridization and PCR amplification. To investigate the possible existence of invasive and noninvasive strains of E. histolytica, the ability to differentiate individual isolates is necessary. For this purpose, we have utilized a modification of the AFLP procedure called 'Transposon display,' which generates and displays large number of genomic bands associated with a transposon. We have used the abundant retrotransposon, EhSINE1, for this purpose, and demonstrated its potential as a marker to study strain variation in E. histolytica. This technique could suitably be employed in carrying out significant molecular epidemiological studies and large-scale typing of this parasite.

show abstract

Section: Isolation Of Dna From Axenic Cultures and Stool Samplesmentioning

confidence: 99%

Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar

Srivastava¹,

Bhattacharya²,

Paul³

2005

Experimental Parasitology

View full text Add to dashboard Cite

show abstract

“…Samples fixed in MIF were processed by the direct MIF and MIF concentration techniques, 24,25 and those fixed in 10% formalin were processed by a formol-ether concentration technique. 26 Four slides per specimen were fully examined by one professional and finally by one of the authors (J-GE). Microscopic slides and materials from the human parasite collection of the Parasitology Department of the University of Valencia were used for quality control when needed.…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

Balantidiasis in Aymara children from the northern Bolivian Altiplano.

Esteban

Aguirre²,

Angles³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Balantidium coli infection was coprologically studied in 2,124 Aymara children 5-19 years of age from the schools of 22 communities of the northern Bolivian Altiplano over a five-year period. Infection with B. coli was found in 11 of the communities surveyed, with prevalences of 1.0-5.3% (overall prevalence ϭ 1.2%). The prevalences observed are some of the highest reported and did not differ significantly among the various age groups or between boys and girls. These prevalences, the apparent absence of symptoms or signs of illness due to this parasite in the schoolchildren surveyed at the time of stool sampling, and the consistency of stool samples of the infected students suggest that they are apparently asymptomatic carriers. Infection with B. coli must be considered as an endemic anthropozoonosis in the area studied. A relationship between B. coli infection and Altiplanic pigs is suggested.Balantidium coli (Malmsten, 1857) (Ciliophora: Balantiidae) is the largest protozoan and the only ciliate parasite of humans. Although numerous animal species are known to harbor B. coli, pigs have a particularly high rate of infection (20-100%) and consequently are generally considered the main natural reservoir of this parasite.

show abstract

“…Fresh faecal samples were processed individually and examined microscopically for helminth eggs and protozoan cysts and/or oocysts after concentration by the modified formol-ether technique 13 .…”

Section: Parasitological Proceduresmentioning

confidence: 99%

Prevalence of gastrointestinal parasites of stray dogs impounded by the Society for the Prevention of Cruelty to Animals (SPCA), Durban and Coast, South Africa : short communication

Mukaratirwa¹,

Singh²

2010

J. S. Afr. Vet. Assoc.

View full text Add to dashboard Cite

Coprological examination was used to determine the prevalence and intensity of gastrointestinal parasites of stray dogs impounded by the Society for the Prevention of Cruelty to Animals (SPCA), Durban and Coast, South Africa. Helminth and protozoan parasites were found in faeces of 240 dogs with an overall prevalence of 82.5% (helminth parasites 93.1% and protozoan parasites 6.9 %). The following parasites and their prevalences were detected; Ancylostoma sp. (53.8 %), Trichuris vulpis (7.9 %), Spirocerca lupi (5.4 %), Toxocara canis (7.9 %), Toxascaris leonina (0.4 %) Giardia intestinalis (5.6 %) and Isospora sp. (1.3 %). Dogs harbouring a single parasite species were more common (41.7 %) than those harbouring 2 (15 %) or multiple (2.1 %) species. Ancylostoma sp., Toxocara canis and Giardia intestinalis have zoonotic potential and were detected in 66.7 % of the samples

show abstract

A Modification of the Formol-Ether Concentration Technique for Increased Sensitivity in Detecting Schistosoma Mansoni Eggs

Cited by 116 publications

References 0 publications

Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar

Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar

Balantidiasis in Aymara children from the northern Bolivian Altiplano.

Prevalence of gastrointestinal parasites of stray dogs impounded by the Society for the Prevention of Cruelty to Animals (SPCA), Durban and Coast, South Africa : short communication

Contact Info

Product

Resources

About