Accurate
discrimination of cancerous cells is a good solution for
early diagnosis of tumors. The mitochondrion plays an important role
in cells. Herein, the five aggregation-induced emission luminogens
(AIEgens) with various double positive charges are synthesized to
image mitochondria. Tetraphenylethylene (TPE) molecules are modified
by methoxy groups, conjugated donor–acceptor, and different
positive charges to achieve multicolor emission. The five AIEgens
form the PTx-Sa (positive mitochondria-target
molecular sensor array) to perform cross-fluorescence response based
on the mitochondria-targeted imaging to achieve the discrimination
of various cells. Principal component analysis of the cross-response
fluorescence data of PTx-Sa shows that
100% accurate identification of various cells, including cancer cells
and normal cells, digestive tract cancer cells, gastric cancer cells,
and mixed gastric cancer cells. By support vector machine to show
the predictive ability of PTx-Sa to
unknown cells by using blind samples. This is the first time to apply
mitochondria-targeted sensor array to identification of various cells.