A Molecular Systematics and Taxonomy Research on Trechispora (Hydnodontaceae, Trechisporales): Concentrating on Three New Trechispora Species from East Asia

Abstract

Trechispora are an important genus of wood-inhabiting fungi that have the ability to decompose rotten wood in the forest ecosystem. In this study, we reported three new species of Trechispora: T. murina, T. odontioidea, T. olivacea from a subtropical region of Yunnan Province, China. Species descriptions were based on a combination of morphological features and phylogenetic analyses of the ITS and LSU region of nuclear ribosomal DNA. Trechispora murina is characterized by the resupinate basidiomata, grandinioid hymenial surface with a greyish tint, monomitic hyphal system and ellipsoid, thick-walled, ornamented basidiospores; T. odontioidea has an odontioid hymenial surface with cylindrical to conical, blunt aculei and subglobose to globose, colorless, slightly thick-walled, ornamented basidiospores; T. olivacea has a farinaceous hymenial surface with olivaceous tint, basidia clavate and thick-walled, ornamented, broadly ellipsoid to globose basidiospores. Sequences of the ITS and nLSU rDNA markers of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. After a series of phylogenetic analyses, the 5.8S+nLSU dataset was constructed to test the phylogenetic relationship of Trechispora with other genera of Hydnodontaceae. The ITS dataset was used to evaluate the phylogenetic relationship of the three new species with other species of Trechispora. Using ITS phylogeny, the new species T. murina was retrieved as a sister to T. bambusicola with moderate supports; T. odontioidea formed a single lineage and then grouped with T. fimbriata and T. nivea; while T. olivacea formed a monophyletic lineage with T. farinacea, T. hondurensis, and T. mollis.

Morphological and molecular identification for four new wood-inhabiting species of Trechispora (Basidiomycota) from China

“…Sequences of Fibrodontia alba Yurchenko & Sheng H. Wu and F. brevidens (Pat.) Hjortstam & Ryvarden retrieved from GenBank were used as an outgroup in the ITS analysis ( Luo and Zhao 2022 ).…”

“… Fungi represent one of the most diverse groups of organisms on earth, with an indispensable role in the processes and functioning of ecosystems ( Wu et al 2020 , 2021 ; Wang et al 2021 ; Hyde 2022 ; Guan and Zhao 2023 ). Wood-inhabiting fungi play an important role in the carbon cycle ( Dai et al 2015 ; Wu et al 2017 ; Chen and Zhao 2020 ; Guan et al 2021 ; Liu et al 2022a , b ; Luo and Zhao 2022 ; Mardones et al 2023 ; Yuan et al 2023 ; Zhang et al 2023 ; Zhao et al 2023a , b ; Zhou et al 2023 ). The wood-inhabiting fungal order Trechisporales K.H.…”

“…Currently, MycoBank and Index Fungorum have registered 163 recorded and 156 recorded intraspecific names in Trechispora , respectively. About 100 species are currently accepted in Trechispora worldwide ( Karsten 1890 ; Bondartsev and Singer 1941 ; Rogers and Jackson 1943 ; Rogers 1944 ; Bondartsev 1953 ; Liberta 1966 , 1973 ; Parmasto 1968 ; Burdsall and Gilbertson 1982 ; Gilbertson and Budington 1970 ; Jülich 1975 , 1976 ; Ryvarden 1975 ; Ryvarden and Liberta 1978 ; Hallenberg 1978 , 1980 ; Jülich and Stalpers 1980 ; Rauschert 1987 ; Vries 1987 ; Larsson 1992 , 1994 , 1995 , 1996 ; Hjortstam and Larsson 1995 ; Ryvarden 2002 ; Trichies and Schultheis 2002 ; Ryvarden et al 2003 ; Miettinen and Larsson 2006 ; Dai 2011 ; Yuan and Dai 2012 ; Ordynets et al 2015 ; Phookamsak et al 2019 ; Xu et al 2019 ; Chikowski et al 2020 ; Haelewaters et al 2020 ; Crous et al 2021 ; de Meiras-Ottoni et al 2021 ; Zhao and Zhao 2021 ; Liu et al 2022a , b ; Luo and Zhao 2022 ; Sommai et al 2023 ), of which 38 species of the genus have been found in China ( Dai 2011 ; Yuan and Dai 2012 ; Xu et al 2019 ; Dai et al 2021 ; Zhao and Zhao 2021 ; He et al 2022 ; Liu et al 2022a , b ; Luo and Zhao 2022 ; Deng et al 2023 ; Liu et al 2024a , b ).…”

“…The phylogeny of Trechisporales was inferred from a combined ITS-nLSU sequences, which revealed that two related genera Porpomyces , Scytinopogon Singer, grouped closely together with Trechispora and all of them nested within Hydnodontaceae ( Liu et al 2019 ). Based on ITS dataset, the three new species of Trechispora were described and used to evaluate the phylogenetic relationship with other species of this genus, in which T. murina was retrieved as a sister to T. bambusicola with moderate supports, and T. odontioidea formed a single lineage and then grouped with T. fimbriata and T. nivea , while T. olivacea formed a monophyletic lineage with T. farinacea , T. hondurensis , and T. mollis ( Luo and Zhao 2022 ). Recently, based on the morphological features and molecular evidence, three new species of Trechispora have been reported from Northern and Northeastern Thailand ( Sommai et al 2023 ).…”

Morphological and molecular identification for four new species of wood-inhabiting macrofungi (Basidiomycota, Hydnodontaceae) from Yunnan-Guizhou Plateau, China

“…The phylogeny of Trechisporales was inferred from a combined ITS-nLSU sequences, which revealed that two related genera Porpomyces, Scytinopogon Singer, grouped closely together with Trechispora and all of them nested within Hydnodontaceae (Liu et al 2019). Based on ITS dataset, the three new species of Trechispora were described and used to evaluate the phylogenetic relationship with other species of this genus, in which T. murina was retrieved as a sister to T. bambusicola with moderate supports, and T. odontioidea formed a single lineage and then grouped with T. mbriata and T. nivea, while T. olivacea formed a monophyletic lineage with T. farinacea, T. hondurensis, and T. mollis (Luo and Zhao 2022a). Recently, based on the morphological features and molecular evidence, three new species of Trechispora has been reported from Northern and Northeastern Thailand (Sommai et al 2023).…”