A multi-laboratory evaluation of cryopreserved monkey hepatocyte functions for use in pharmaco–toxicology

Sousa, Georges de; Nicolas, Frédéric; Placidi, M; Rahmani, Roger; Benicourt, Marc; Vannier, Bernard; Lorenzon, Giocondo; Mertens, Karin; Coecke, Sandra; Callaerts, A.; Rogiers, Vera; Khan, Shamas; Roberts, P.; Skett, Paul; Fautrel, Alain; Chesné, Christophe

doi:10.1016/s0009-2797(99)00092-7

Cited by 15 publications

(6 citation statements)

References 46 publications

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“…Temperature, in general, is well recognized to impact viral infectivity during transport and/or storage. As observed in the current study, isolation of spumavirus from five of eight diluents after 7 days at 4°C, compared with one isolate (i.e., virus maintained in EMEM‐10%) at 23°C, concurs with the recognized protective effect of refrigeration [8,14]. Organic constituents in the media, such as sucrose or FBS (i.e., the sucrose component in CTM; the FBS supplement in EMEM), further served as viral stabilizing agents [15,16].…”

Section: Resultssupporting

confidence: 82%

“…Persistent spumavirus infections in primates sporadically result in the adulteration of cell cultures prepared from organs obtained from this animal group. The extensive use of primate cell cultures in human and veterinary diagnostics, their potential use in toxicologic research, and the utilization of primates themselves in numerous research disciplines denote a need to identify further those physical factors that might interplay in viral particle behavior (i.e., persistence/loss of infectivity) in the laboratory or clinical settings (see [8,9]).…”

Section: Introductionmentioning

confidence: 99%

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Survival of spumavirus, a primate retrovirus, in laboratory media and water

Lotlikar

Lipson

2002

FEMS Microbiology Letters

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The persistence of a previously characterized spumavirus strain (strain SV-522) was investigated utilizing various laboratory media and waters, including Eagle's minimal essential medium (EMEM) plus 0% fetal bovine serum (EMEM-0%), EMEM-2%, EMEM-10%, Chlamydia transport medium (CTM), phosphate-buffered saline, distilled, estuarine, and marine water, human serum, and the germicides, ethyl alcohol (70%) and sodium hypochlorite (10%). Experiments were performed at 4 degrees C and/or 23 degrees C. Infectivity endpoints were determined in stock aliquots upon initiation of testing and then after 3, 5, 7, and 10 days. The virus was reisolated from all diluents after 5 days at 23 degrees C and in EMEM-10% after 7 days. The virus was detected in CTM, EMEM-2%, EMEM-10%, and estuarine and marine waters after 7 days at 4 degrees C. Differences in the persistence of the virus may be ascribed to temperature and organic load. Water ionic strengths (e.g., estuarine vs. marine water) had no effect on modifying persistence of viral particles. Infectivity of spumavirus was undetectable after 30 s in 70% ethanol or 10% sodium hypochlorite. After 30 min at 23 degrees C, spumavirus infectivity in normal but not heat-inactivated human serum increased by almost 100-fold. Persistence of infectivity of primate spumavirus after 7 days in media and waters, and the agent's infectious potential in the human host, emphasize a need for cautious recognition during the manipulation of primate cells/organs and in the handling of primates themselves.

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Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Lotlikar

Lipson

2002

FEMS Microbiology Letters

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“…Hepatocyte cryopreservation has primarily focused on suspension freezing using medium (such as University of Wisconsin, Williams E medium, or Leibowitz L15 medium) supplemented with 10-40% FBS and 10-20% DMSO. 58,71,74,[77][78][79] Reports on post-thaw function vary significantly, with urea secretion, phase I and II metabolism, albumin/protein synthesis, and ATP content ranging from unchanged to 14% of the values expected from fresh hepatocytes. Therefore, in this study, mouse hepatocyte monolayers cryopreserved with 10% DMSO and IN were evaluated for critical hepatic function 24 hours post-thaw and compared to non-frozen hepatocytes.…”

Section: Papermentioning

confidence: 99%

Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities

Tomás,

Dallman,

Congdon

et al. 2023

Biomater. Sci.

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“…To guarantee the survival of hepatocytes isolated from individual donors, cryopreservation or cold storage techniques can be applied that lead to an inde nite (23,29) or 48-hour (54, 77) extension, respectively. However, the viability of stored cells is much lower than that of freshly isolated hepatocytes and dependent on factors such as initial cell integrity, ice crystal formation, and hypoxia during freezing and toxicity of cryopreservation substances.…”

Section: Isolated Liver Cell Modelsmentioning

confidence: 99%

In Vitro Models to Study Hepatotoxicity

Groneberg¹,

Große-Siestrup

Fischer³

2002

Toxicol Pathol

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Drug discovery and developmen t consists of a series of processes starting with the demonstration of pharmacologica l effects in experimental cell and animal models and ending with drug safety and ef cacy studies in patients. A main limitation is often the unacceptabl e level of toxicity with the liver as the primary target organ. Therefore, approache s to study hepatic toxicity in the early phase of drug discovery represent an important step towards rational drug development . A variety of in vitro liver models have been developed in the past years. Next to their use in drug development , they can also be applied to study environmenta l toxins and their hepatotoxicity. The 3 main approache s are ex vivo isolated and perfused organ models, precision-cut liver slices and cell culture models. Although the advantage of whole organ perfusions is based on the assessment of physiologic parameters such as bile production and morphologi c parameters such as tissue histology, cell culture models can be ef ciently used to assess cellular metabolism, cytotoxicity and genotoxicit y. The advantage of precision-cut liver slices is based on the juxtaposition of cellular assays and tissue morpholog y. None of these models can be compared as they all focus on different elds of hepatoxicolog y. For the future, the ideal setup for testing the hepatic toxicity of a new compoun d could of primary studies in cell or slice cultures to assess cellular effects and secondary studies using ex vivo perfused organs to examine gross organ function parameters and histology.

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A multi-laboratory evaluation of cryopreserved monkey hepatocyte functions for use in pharmaco–toxicology

Cited by 15 publications

References 46 publications

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Survival of spumavirus, a primate retrovirus, in laboratory media and water

Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities

In Vitro Models to Study Hepatotoxicity

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