A multilayer coil in type-I counter-current chromatography

Abstract

In the present study, a novel model of type-I counter-current chromatography was developed to simplify the separation column manufacture and extend its application ranges. In this system, a multilayer coil was used as the separation column of type-I counter-current chromatography for the first time. The chromatographic performance of this apparatus was evaluated in terms of retention of the stationary phase (Sf), theoretical plate (N) and peak resolution (Rs) using dipeptides and DNP-amino acids as test samples with two classic solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v) (BAW) and hexane-ethyl acetate-methanol-0.1 M HCl (1:1:1:1, v/v) (HEMW), respectively. The results indicated that the performance was optimal at the revolution speed of 200 rpm. The lower flow rate was more beneficial to retention of stationary phase and peak resolution. Over all results of our study revealed that the new type-I counter-current chromatography with a multilayer coil has a high application potential.

Development of online-storage inner-recycling counter-current chromatography for the preparative separation of complex components of alkylphenols from sarcotesta of Ginkgo biloba L.

“…This may be expressed by the following equation: ( R 1 / R 2 ) 2 = N 1 / N 2 = L 1 / L 2 where R is the resolution, N is the total number of theoretical plates, and L is the length of the column. 28 …”

“…where R is the resolution, N is the total number of theoretical plates, and L is the length of the column. 28 For a particular CCC instrument, the number of theoretical plates in a one-step separation is limited. Compared with HPLC, the number of theoretical plates of HSCCC is low, resulting in inferior separation.…”