A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis

Sarauli, David; Xu, Cheng; Dietzel, Birgit; Schulz, Burkhard; Lisdat, Fred

doi:10.1039/c4tb00336e

Cited by 21 publications

(22 citation statements)

References 57 publications

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“…The methoxylation leads to an electron donating effect within the polymer and therefore increases its electron density [ 46 ]. Methoxylated polyanilines have already been used in biosensors and could improve electron transfer (ET) [ 47 ]. The carboxylation makes the polymer soluble and it can also be used to couple the polymer to the surface of amino-functionalities.…”

Section: Resultsmentioning

confidence: 99%

Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes

et al. 2014

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Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose.

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Section: Resultsmentioning

confidence: 99%

Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes

et al. 2014

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“…The hybridization of DNA probes with the target (analyte) DNA was determined, evaluating the charge transfer resistance. Also, the formation of multilayers by the combination of polymers and enzymes in biosensing schemes has been evaluated using a simple Randles circuit [45].…”

Section: Elementmentioning

confidence: 99%

Electrochemical techniques for investigating redox active macromolecules

Izquierdo

Kranz

2016

European Polymer Journal

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“…The treatment related to anti-fibrosis remained an unconquered era for drug progression, development.PQQ via scavenging activity can open new horizons in this field [145]. A significant protection in liver and colon cells was found with administration of PQQ [146] and to extricate untimely senescence, provoked by eradication of Bmi-1 and inhibition of oxidative stress by ROS can cause liver impairment [147]. Multiple lines of evidence indicate that NOX-generated ROS engaged in crucial function of liver pathogenesis [148].…”

Section: Pqq In Liver Fibrogensismentioning

confidence: 99%

The Life History of Pyrroloquinoline Quinone (PQQ): A Versatile Molecule with Novel Impacts on Living Systems

Naveed¹

2016

IJMBOA

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Pyrroloquinoline quinone (PQQ) acting as redox cofactor of Glucose dehydrogenase is orthocyclic antioxidant acting under multifarious environmental stress and rich conditions in prokaryotes as well as eukaryotes. Microbes are the exclusive source of PQQ biosynthesis and for biocatalysis of glucose into gluconic acid and 2-ketogluconic acid by gram positive and negative bacteria. This study focus to describe PQQ biography highlighted its applications acts as biocatalyst by enhanced production of NADH from pqqC, involved in several important mechanisms like bacterial energy transduction by m-ATPase, production of biocontrol substances, growth stimulating activities and DNA repair. PQQ acting as anti-neurological, anti-degenerative, anti-melanogenic and anti-cancer agent due to its antioxidant nature by scavenging free radicals. PQQ is modulator of immunity by CD4 cells count and IL-2, sleep maintenance by PGC-1 alpha pathway and inflammation due to ROS. The mineral phosphate solubilization results in plant growth promotional, biocontrol, antifungal and ISR activities. PQQ nanoparticles used for production of Biofuels cells and sulfonated polymers. It acts as a modulator of diverse signaling pathways like STAT, MAPK, JAK, JNK, P13K/Akt, mTOR, EGFR and Raps for cell proliferation, differentiation, apoptosis, Box translocation and metabolic pathways by phosphorylation of ADP and suppression of reactive oxygen species. It protects from oxidative stress by acting as Alpha AA modulator of lysine metabolism, TrxR1 in selenium metabolism, low density lipoprotein in lipid metabolism, ATP production in Energy, Glucose and carbohydrate metabolism. It has been structurally characterized with bioinformatics tools and future perspectives being molecular modeling and docking for analytical drug design.

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A multilayered sulfonated polyaniline network with entrapped pyrroloquinoline quinone-dependent glucose dehydrogenase: tunable direct bioelectrocatalysis

Abstract: Differently sulfonated polyaniline copolymers have been utilized as matrices for the entrapment of PQQ-GDH, resulting in a direct bioelectrocatalytic response together with a colour change upon addition of the substrate.

Cited by 21 publications

References 57 publications

Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes

Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes

Electrochemical techniques for investigating redox active macromolecules

The Life History of Pyrroloquinoline Quinone (PQQ): A Versatile Molecule with Novel Impacts on Living Systems

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