A multiplex panel of short-amplicon insertion-deletion DNA polymorphisms for forensic analysis

Abstract

ABSTRACT.We have previously developed a panel of 40 insertiondeletion (INDEL) human DNA polymorphisms that was proven to adequately cover the span of global human genetic diversity. The panel was found to have very low matching probabilities with respect to both the global and Brazilian populations. To optimize the panel for application with degraded DNA samples, which are commonly encountered in forensic analysis, we have significantly reduced the amplicon size of the INDELs and developed a new multiplex panel. The panel has an amplicon size ranging from 50 to 153 base pairs, with a mean of 93 base pairs. It could be amplified by polymerase chain reaction in two multiplex reactions, which were then combined for electrophoretic separation and identification of the individual products in the ABI3130 four-color DNA analyzer. The results of the new panel were fully validated.

Development of a novel multiplex polymerase chain reaction system for forensic individual identification using insertion/deletion polymorphisms

“…Besides, amplicon sizes of 35 InDels ranged from 104 to 304 bp. Compared to amplicon sizes of published InDels , some out of the 35 InDels possessed relatively larger amplicon sizes. Further validation evaluations of forensic efficiencies, such as the ability to analyze degraded samples, sensitivity, specificity, stability, and so on, should be performed in the latter work.…”