A multiplexed point-of-care assay for C-reactive protein and N-terminal pro-brain natriuretic peptide

Melin, Jonas; Rundström, Gerd; Peterson, Christer; Bakker, Jimmy; MacCraith, Brian D.; Read, Mike; Öhman, Ove; Jönsson, Christina

doi:10.1016/j.ab.2010.09.034

Cited by 14 publications

(6 citation statements)

References 17 publications

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“…We clearly observed a further decrease in the Ang_II levels after one more week of treatment, and the much higher decreases are supported by the P-values 1.73e-33 and 5.01e-12, respectively. High sensitivity C-reactive protein (hs_CRP) is a protein whose serum level is positively correlated with the inflammation in the body, and amino terminal pro-B-type natriuretic peptide (NT_proBNP) is the N-termini of the brain natriuretic peptide whose serum level is positively associated with the CHF severeness 39. The serum levels of both molecules are used as markers to describe how severe the cardiac risk is 39.…”

Section: Resultsmentioning

confidence: 99%

“…High sensitivity C-reactive protein (hs_CRP) is a protein whose serum level is positively correlated with the inflammation in the body, and amino terminal pro-B-type natriuretic peptide (NT_proBNP) is the N-termini of the brain natriuretic peptide whose serum level is positively associated with the CHF severeness 39. The serum levels of both molecules are used as markers to describe how severe the cardiac risk is 39. The continuous decreases in the two molecular levels after the standard treatment in days 7 and 15 suggest that the treatment works reasonably well on CHF, as shown in Table 3.…”

Section: Resultsmentioning

confidence: 99%

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Clinical Effects of Xinmailong Therapy in Patients with Chronic Heart Failure

Ma¹,

Luo²,

Guo³

et al. 2013

Int. J. Med. Sci.

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In the last 100 years, intensive studies have been done on the identification of the systematic approaches to find the cure for the chronic heart failure, however the mystery remains unresolved due to its complicated pathogenesis and ineffective early diagnosis. The present investigation was aimed to evaluate the potential effects of the traditional chinese medicine, Xinmailong, on the chronic heart failure (CHF) patients as compared to the standard western medical treatment available so far. In our study, we selected two groups of voluntary CHF patients at the Xiangya Hospital, which were allowed to administrate Xinmailong or standard treatments, respectively. Another group of voluntary healthy individuals were recruited as the control group. The treatment effectiveness was measured by five symptomatic factors, i.e. angiotensin II (Ang_II), high sensitivity C-reactive protein (hs_CRP), Left Ventricular End Systolic Volume Index (LVESVI), left ventricular ejection fraction (LVEF) and pro-B-type natriuretic peptide (NT_proBNP), between the control group and the CHF patients at different stages of drug administration and in different treatment groups. The timeline for the full dose administration was set to 15 days and five measurements as indicated above were taken on every 0, 7th and 15th day of the drug administration respectively. In the conducted study, similar symptomatic measurements were observed on day 0 in both treatment groups, and slight improvements were observed on 7th day. It was observed that after a full course of drug administration for 15 days, both of the treatment groups achieved statistically significant improvements in all the five measures, but Xinmailong was found to be more (almost double) statistically significant as compared with the available drug treatments for chronic heart failure.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Clinical Effects of Xinmailong Therapy in Patients with Chronic Heart Failure

Ma¹,

Luo²,

Guo³

et al. 2013

Int. J. Med. Sci.

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“…Electrocardiograms (ECG), the first test when a patient suffering from chest pain goes to the doctor, lack of the required sensitivity. During recent years, several protein biomarkers have been associated with increased relative risk for cardiovascular events and could potentially add predictive value to establish risk factor sets 1. Standard assays to detect cardiac biomarkers, like enzyme‐linked immunosorbent assay (ELISA), are sensitive but suffer from important sample and reagent consumption in large‐scale studies.…”

Section: Introductionmentioning

confidence: 99%

“…Amino‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) and C‐reactive protein (CRP) are considered as two very promising biomarkers for cardiac risk prediction 1, 11. However, the clinically relevant cut‐off concentrations for NT‐proBNP and CRP differ by three orders of magnitude, and in extreme cases their serum concentrations could in principle differ by more than six or seven orders of magnitude 1, making their simultaneous detection in the same sample using the same device without different sample dilutions to cluster analytes into narrower concentration ranges, challenging.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Determination of Amino‐Terminal Pro‐B‐Type Natriuretic Peptide and C‐Reactive Protein Cardiac Biomarkers in Human Serum at a Disposable Electrochemical Magnetoimmunosensor

et al. 2013

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A rapid magnetoimmunosensor for the simultaneous determination of two cardiac biomarkers, amino‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP) and C‐reactive protein (CRP), in human serum is described. Specific capture antibodies were covalently immobilized onto carboxylic acid‐modified magnetic beads. The quantification of NT‐proBNP and CRP was performed by using indirect competitive and sandwich configurations, respectively, and horseradish peroxidase‐labeled tracers. The use of dual screen‐printed carbon electrodes allowed the achievement of simultaneous independent amperometric readout for each cardiac biomarker. The developed methodology showed very low limits of detection (0.47 ng mL−1). An international standard for CRP serum spiked with NT‐proBNP was analyzed to evaluate the usefulness of the magnetoimmunosensor.

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“…The flow rate of PBS was about 4 mm/s on the chip. In this experiment, the design of microchip referred to the microstructure of micropost array of 4castchip ® developed by Åmic AB [17,18]. It is important to note that the LF strip is one of the most successful commercial POCT products.…”

Section: Resultsmentioning

confidence: 99%

Capillary-driven surface-enhanced Raman scattering (SERS)-based microfluidic chip for abrin detection

Yang

Deng

et al. 2014

Nanoscale Res Lett

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Herein, we firstly demonstrate the design and the proof-of-concept use of a capillary-driven surface-enhanced Raman scattering (SERS)-based microfluidic chip for abrin detection. The micropillar array substrate was etched and coated with a gold film by microelectromechanical systems (MEMS) process to integrate into a lateral flow test strip. The detection of abrin solutions of various concentrations was performed by the as-prepared microfluidic chip. It was shown that the correlation between the abrin concentration and SERS signal was found to be linear within the range of 0.1 ng/mL to 1 μg/mL with a limit of detection of 0.1 ng/mL. Our microfluidic chip design enhanced the operability of SERS-based immunodiagnostic techniques, significantly reducing the complication and cost of preparation as compared to previous SERS-based works. Meanwhile, this design proved the superiority to conventional lateral flow test strips in respect of both sensitivity and quantitation and showed great potential in the diagnosis and treatment for abrin poisoning as well as on-site screening of abrin-spiked materials.

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A multiplexed point-of-care assay for C-reactive protein and N-terminal pro-brain natriuretic peptide

Cited by 14 publications

References 17 publications

Clinical Effects of Xinmailong Therapy in Patients with Chronic Heart Failure

Clinical Effects of Xinmailong Therapy in Patients with Chronic Heart Failure

Multiplexed Determination of Amino‐Terminal Pro‐B‐Type Natriuretic Peptide and C‐Reactive Protein Cardiac Biomarkers in Human Serum at a Disposable Electrochemical Magnetoimmunosensor

Capillary-driven surface-enhanced Raman scattering (SERS)-based microfluidic chip for abrin detection

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