A multiplexed protein microarray for the simultaneous serodiagnosis of human immunodeficiency virus/hepatitis C virus infection and typing of whole blood

Burgess, Stewart T. G.; Kenyon, Fiona; O'Looney, Nichola; Ross, Alan J.; Kwan, Marisa Chong; Beattie, John S.; Petrík, Juraj; Ghazal, Peter; Campbell, Colin J.

doi:10.1016/j.ab.2008.07.017

Cited by 21 publications

(18 citation statements)

References 17 publications

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“…In addition to DNA microarrays, interrogating host antibody responses by the use of protein microarrays is feasible for multiplexed detection of blood‐borne pathogens . Protein arrays containing >40 000 peptides have been developed to detect infection from a variety of targeted infectious agents, including viruses, bacteria and parasites . The arrays are typically developed by specific PCR amplification of the expressed genes within the genomes of targeted infectious agents, transformation of the PCR amplicons into bacterial plasmids and then in vitro transcription and automated printing of the protein arrays as individual spots onto glass slides.…”

Section: Testing In the Futurementioning

confidence: 99%

Advances in testing technology to ensure transfusion safety – NAT and beyond

Stramer

Dodd

Chiu

2015

ISBT Science Series

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Aims To provide an update on state-of-the-art testing technologies for infectious disease (ID) blood donation screening, including emerging molecular methods such as multiplexed nucleic acid testing (NAT) platforms, mass spectrometry, nucleic acid and protein microarrays, and next-generation sequencing.Background Testing donated blood for ID markers plays a major role in establishing and maintaining blood safety. The norm for ID testing has been serology, but increasingly, NAT is used where resources allow, including testing for emerging infectious disease (EID) agents. The appropriate mix of ID tests and associated algorithms depends on local epidemiology, infrastructure and economic considerations. Although NAT will detect acute infections, not all pathogens have adequate concentrations of nucleic acid for detection in the early acute or later chronic phases of infection. Thus, both NAT and serological approaches may be required to detect recent, current or past infection. The advent of new technologies to augment or potentially replace current NAT and/or serologic testing has spurred debate as to their optimal future role in blood-borne pathogen screening. Methods and ResultsInfectious disease testing has progressed from manual technologies using single markers to highly multiplexed automated assays. Emerging technologies allow the simultaneous detection and differentiation of hundreds of pathogens and will likely play an important role in future testing, but there are many hurdles prior to routine adoption.Conclusions Blood systems worldwide must be ready to adapt to EID agents, availability of new diagnostic technologies, and shifts in economic conditions and public expectations to accommodate the changing landscape of ID testing.

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Section: Testing In the Futurementioning

confidence: 99%

Advances in testing technology to ensure transfusion safety – NAT and beyond

Stramer

Dodd

Chiu

2015

ISBT Science Series

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“…All of the proteins which are found in high abundance in VSV were detected demonstrating that redundant measurement of unique viral components could provide a way to identify pathogens given the possibility of antigenic mutation (ex: antigenic shift/drift) or genetic modification The use of many specific (monoclonal) antibodies in a microarray format could allow for serodiagnosis and direct pathogen detection of patient samples [Burgess et al, 2008]. Additionally, these results support the use of this platform with complex solutions as the current surface chemistry and sample preparation procedures for antigen binding provided specific detection.…”

Section: Discussionmentioning

confidence: 99%

Label-free multiplexed virus detection using spectral reflectance imaging

Lopez

Daaboul

Vedula

et al. 2011

Biosensors and Bioelectronics

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We demonstrate detection of whole viruses and viral proteins with a new label-free platform based on spectral reflectance imaging. The Interferometric Reflectance Imaging Sensor (IRIS) has been shown to be capable of sensitive protein and DNA detection in a real time and high-throughput format. Vesicular stomatitis virus (VSV) was used as the target for detection as it is wellcharacterized for protein composition and can be modified to express viral coat proteins from other dangerous, highly pathogenic agents for surrogate detection while remaining a biosafety level 2 agent. We demonstrate specific detection of intact VSV virions achieved with surfaceimmobilized antibodies acting as capture probes which is confirmed using fluorescence imaging. The limit of detection is confirmed down to 3.5×10 5 plaque-forming units/mL (PFUs/mL). To increase specificity in a clinical scenario, both the external glycoprotein and internal viral proteins were simultaneously detected with the same antibody arrays with detergent-disrupted purified VSV and infected cell lysate solutions. Our results show sensitive and specific virus detection with a simple surface chemistry and minimal sample preparation on a quantitative label-free interferometric platform.

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“…Incubation of ELISA plates with biotinylated detecting antibody cocktails was performed in PBS (containing 0.1% Tween-20 and 5% FCS) at 37°C for 2 h. After washing, specific binding of the detecting antibodies was reported by streptavidin-HRP and stained with Super Signal ELISA Femto Maximum sensitive substrate (Thermo scientific, Rockford, USA) [11,14,15]. Visualization of the plate was performed in AE 1000 cool CCD image analyzer(Beijing BGI GBI Biotech Company., LTD, China).…”

Section: Methodsmentioning

confidence: 99%

Development of an ELISA-array for simultaneous detection of five encephalitis viruses

Kang

Fan

et al. 2012

Virol J

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Japanese encephalitis virus(JEV), tick-borne encephalitis virus(TBEV), and eastern equine encephalitis virus (EEEV) can cause symptoms of encephalitis. Establishment of accurate and easy methods by which to detect these viruses is essential for the prevention and treatment of associated infectious diseases. Currently, there are still no multiple antigen detection methods available clinically. An ELISA-array, which detects multiple antigens, is easy to handle, and inexpensive, has enormous potential in pathogen detection. An ELISA-array method for the simultaneous detection of five encephalitis viruses was developed in this study. Seven monoclonal antibodies against five encephalitis-associated viruses were prepared and used for development of the ELISA-array. The ELISA-array assay is based on a "sandwich" ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that the developed ELISA-array is sensitive and easy to use, which would have potential for clinical use.

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A multiplexed protein microarray for the simultaneous serodiagnosis of human immunodeficiency virus/hepatitis C virus infection and typing of whole blood

Cited by 21 publications

References 17 publications

Advances in testing technology to ensure transfusion safety – NAT and beyond

Advances in testing technology to ensure transfusion safety – NAT and beyond

Label-free multiplexed virus detection using spectral reflectance imaging

Development of an ELISA-array for simultaneous detection of five encephalitis viruses

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