A nanopillar array on black titanium prepared by reactive ion etching augments cardiomyogenic commitment of stem cells

Ghosh, Lopamudra Das; Hasan, Jafar; Jain, Aditi; Sundaresan, Nagalingam R.; Chatterjee, Kaushik

doi:10.1039/c9nr03424b

Cited by 15 publications

(15 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, bactericidal bTi has been introduced as a promising biomaterial for preventing IAIs. [ 48 ] To date, numerous studies [ 46,47,50 ] have focused on the biological properties of bSi and bTi surfaces, trying to optimize the dimensions of the pillars by changing one single processing parameter (i.e., etching time). It was reported that fine changes in the characteristics of the such etched structures may influence their biological properties.…”

Section: Discussionmentioning

confidence: 99%

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Modaresifar

Ganjian

Angeloni

et al. 2021

Small

View full text Add to dashboard Cite

Despite the potential of small‐scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each featuring different pillar designs. The interactions of the surfaces with MC3T3‐E1 preosteoblast cells and Staphylococcus aureus bacteria are then investigated. Pillars with different heights and spatial organizations differently influence the morphological characteristics of the cells, including their spreading area, aspect ratio, nucleus area, and cytoskeletal organization. The preferential formation of focal adhesions (FAs) and their size variations also depend on the type of topography. When the pillars are neither fully separated nor extremely tall, the colocalization of actin fibers and FAs as well as an enhanced matrix mineralization are observed. However, the killing efficiency of these pillars against the bacteria is not as high as that of fully separated and tall pillars. This study provides a new perspective on the dual‐functionality of bTi surfaces and elucidates how the surface design and fabrication parameters can be used to achieve a surface topography with balanced bactericidal and osteogenic properties.

show abstract

Section: Discussionmentioning

confidence: 99%

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Modaresifar

Ganjian

Angeloni

et al. 2021

Small

View full text Add to dashboard Cite

show abstract

“…28 Ti nano pillar surfaces, engineered by maskless plasma etching, were recently reported to promote both the osteogenic and cardiomyogenic lineage differentiation of mesenchymal stem cells. 31,32 In this context, it seemed attractive to analyze the potential osteoconductive properties of mechano-bactericidal Ti surface patterns established in our previous works. Here we aimed to investigate the adhesion, growth and differentiation ability of human adipose-derived mesenchymal stem cells (hASCs) in response to two types of mechano-bactericidal Ti topographies.…”

Section: Introductionmentioning

confidence: 99%

Mechano-Bactericidal Titanium Surfaces for Bone Tissue Engineering

Clainche

Linklater

Wong

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Despite advances in the development of bone substitutes and strict aseptic procedures, the majority of failures in bone grafting surgery are related to nosocomial infections. Development of biomaterials combining both osteogenic and antibiotic activity is, therefore, a crucial public health issue. Herein, two types of intrinsically bactericidal titanium supports were fabricated by using commercially scalable techniques: plasma etching or hydrothermal treatment, which display two separate mechanisms of mechano-bactericidal action. Hydrothermal etching produces a randomly nanostructured surface with sharp nanosheet protrusions killing bacteria via cutting of the cell membrane, whereas plasma etching of titanium produces a microscale two-tier hierarchical topography that both reduce bacterial attachment and rupture those bacteria that encounter the surface. The adhesion, growth, and proliferation of human adipose-derived stem cells (hASCs) on the two mechano-bactericidal topographies were assessed. Both types of supports allowed the growth and proliferation of the hASCs in the same manner and cells retained their stemness and osteogenic potential. Furthermore, these supports induced osteogenic differentiation of hASCs without the need of differentiation factors, demonstrating their osteoinductive properties. This study proves that these innovative mechano-bactericidal titanium surfaces with both regenerative and bactericidal properties are a promising solution to improve the success rate of reconstructive surgery.

show abstract

“…It is believed that increase in the surface roughness of biomaterials can effectively reinforce cell adhesion, thereby improving the effectiveness of engineered scaffolds. Many biocompatible compounds, such as polyurethane acrylate, [ 10a ] polylactic acid (PLA), [ 34 ] hydroxyapatite (HAp), [ 35 ] titanium (Ti), [ 36 ] and polydimethylsiloxane (PDMS) [ 37 ] have been developed to fabricate or modify biomaterial surfaces to determine cell morphology, adhesion, and metabolism for orienting cell differentiation. For instance, Yang et al.…”

Section: Biophysical Cues Of Biomaterials For Regulating Stem Cell Behaviormentioning

confidence: 99%

“…Various organic macromolecular polymers have been exploited for the development of pillar structures, such as PLA, [ 34 ] polycaprolactone (PCL), [ 50 ] and polystyrene (PS). [ 51 ] Biomaterials with pillar‐like characteristics have already been developed to investigate their effects on stem cells, including MSCs, [ 36 ] human adipose‐derived stem cells (hADSCs), [ 34 ] hematopoietic stem cells, [ 45 ] embryonic stem cells (ESCs), [ 51 ] and human neural stem cells (NSCs). [ 52 ] Pillars with high AR from the nanoscale to the microscale are inclined to reduce cell spreading but enhance elongation and differentiation.…”

Section: Biophysical Cues Of Biomaterials For Regulating Stem Cell Behaviormentioning

confidence: 99%

“…[ 52 ] Pillars with high AR from the nanoscale to the microscale are inclined to reduce cell spreading but enhance elongation and differentiation. [ 34,36,51 ] Gadegaard et al. demonstrated an enhanced induction of definitive endoderm cells from hESCs on the soft polycarbonate pillar arrays with high AR, in contrast to an increased tendency toward pancreatic lineage differentiation on the stiff pillar and planar control.…”

Section: Biophysical Cues Of Biomaterials For Regulating Stem Cell Behaviormentioning

confidence: 99%

See 1 more Smart Citation

Manipulation of Stem Cells Fates: The Master and Multifaceted Roles of Biophysical Cues of Biomaterials

Wan

Liu

2021

Adv Funct Materials

View full text Add to dashboard Cite

Owing to their self‐renewal and differentiation ability, stem cells are conducive for repairing injured tissues, making them a promising source of seed cells for tissue engineering. The extracellular microenvironment (ECM) is under dynamic mechanical control, which is closely related to stem cell behaviors. During the design and fabrication of biomaterials for regenerative medicine, the physiochemical properties of the natural ECM should be closely mimicked, which can reinforce stem cell lineage choice and tissue engineering. By reproducing the biophysical stimulations that stem cells may experience in vivo, many studies have highlighted the key role of biophysical cues in regulation of cell fate. Optimization of biophysical factors leads to desirable stem cell functions, which can maximize the effectiveness of regenerative treatment. In this review, the main biophysical cues of biomaterials, including stiffness, topography, mechanical force, and external physical fields are summarized, and their individual and synergistic influence on stem cell behavior is discussed. Subsequently, the current progress in tissue regeneration using biomaterials is presented, which directs the design and fabrication of functional biomaterial. The mechanisms via which biophysical cues activate cellular responses are also analyzed. Finally, the challenges in basic research as well as for clinical translation in this field are discussed.

show abstract

A nanopillar array on black titanium prepared by reactive ion etching augments cardiomyogenic commitment of stem cells

Abstract: The array highly efficiently promotes cardiomyogenic commitment of stem cells via integrin-mediated signalling compared to the smooth surface and is a potential platform for ex vivo differentiation of stem cells for cell therapy in cardiac tissue repair and regeneration.

Cited by 15 publications

References 61 publications

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual‐Functionality

Mechano-Bactericidal Titanium Surfaces for Bone Tissue Engineering

Manipulation of Stem Cells Fates: The Master and Multifaceted Roles of Biophysical Cues of Biomaterials

Contact Info

Product

Resources

About