A narrative review of urinary phospholipids: from biochemical aspect towards clinical application

High level of phosphatidylcholines/lysophosphatidylcholine ratio in urine is associated with prostate cancer

“…In our previous report, we have defined a quick, qualitative, and quantitative analysis of urinary LPLs and PLs using MALDI‐TOF/MS, as the “qShot MALDI” analysis. 4 Briefly, 10 mmol/L of 9‐AA dissolved in a mixture of IPA and ACN (60:40, v:v) was used as the matrix; and a specially designed target plate, the μFocus MALDI plate (Hudson Surface Technology, Inc), was used to form a precise and concentrated distribution of the sample. Meanwhile, iSTDs containing LPC (17:1) and PC (24:0/24:0) were spiked into the sample before extraction to improve the reproducibility between different wells and on different days.…”

“… 1 , 2 , 3 Among lipid categories, phospholipids (PLs) and lysophospholipids (LPLs) are the most extensively studied. 4 The expression patterns of several PLs and LPLs reportedly differ in prostate cancer (CaP) cells 5 and tissues. 6 Recently, reports have emerged on trained canines being able to detect CaP by sniffing urine samples from these patients.…”

Electron ionization mass spectrometry feature peak relationships combined with deep classification model to assist similarity algorithm for fast and accurate identification of compounds

“…For electron ionization MS using chromatographic separation of substances, molecular samples in the ion source are ionized into ions, through the mass analyzer, that is, detector after the mass spectrometer signals and input are fed into the computer; finally, the unknown compounds and the presence of compounds in the library for the similarity of the matching are used to identify the compounds. 5 The mass spectra of different compounds are often very similar, and the mass spectra of the same compound are not ideally reproducible. Therefore, in many cases, algorithms produce nonideal results even if the compound exists in the database.…”