A Network Thermodynamic Model of Kidney Perfusion

Lachenbruch, Charles A.; Diller, Kenneth R.

doi:10.1016/s1474-6670(17)46240-9

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…As already mentioned, the sinusoidal endothelium is penetrated by large pores and small fenestrae, leading some to term the sinusoid as discontinuous (68). Additionally, typical capillary wall hydraulic permeabilities (including basement membrane and endothelium) exceed cell membrane hydraulic permeabilities by an order of magnitude (69). Thus, it is expected that the cell membrane is the rate-limiting resistance to water transport.…”

Section: Krogh Model Assumptionsmentioning

confidence: 99%

Quantitative Measurement and Prediction of Biophysical Response During Freezing in Tissues

Bischof

2000

Annu. Rev. Biomed. Eng.

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Cryopreservation and cryosurgery are important biomedical applications used to selectively preserve or destroy cellular systems through freezing. Studies using cryomicroscopy techniques, which allow the visualization of the freezing process in single cells, have shown that a drop in viability correlates with the extent of two biophysical events during the freezing process: (a) intracellular ice formation and (b) cellular dehydration. These same biophysical events operate in tissue systems; however, the inability to visualize and quantify the dynamics of the freezing process in tissues has hampered direct correlation of these events with freezing-induced changes in viability. This review highlights two new techniques that use freeze substitution and differential scanning calorimetry to provide dynamic freezing data in tissue. Characteristic dimensions and parameters extracted from these new data are then used in a predictive model of biophysical freezing response in several tissues, including liver and tumor. This approach promises to help guide improved design of both cryopreservation and cryosurgical applications of tissue freezing.

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Section: Krogh Model Assumptionsmentioning

confidence: 99%

Quantitative Measurement and Prediction of Biophysical Response During Freezing in Tissues

Bischof

2000

Annu. Rev. Biomed. Eng.

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“…Historically, efforts to understand CPA loading and unloading and CPA toxicity have been conducted separately, as shown in Table 1 . For instance, there are various models for CPA transport in biological organs, such as the Krogh model [ 37 ], multi-dimensional model [ 28 ], network thermodynamic model [ 26 ], and others. There are also several approaches to understanding CPA toxicity, such as the solution effect model [ 5 , 30 ] ( qv * model [ 9 ]), CPA interaction model [ 21 ], and toxicity cost function model [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Model-Guided Design and Optimization of CPA Perfusion Protocols for Whole Organ Cryopreservation

et al. 2023

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Vitrification could enable long-term organ preservation, but only after loading high-concentration, potentially toxic cryoprotective agents (CPAs) by perfusion. In this paper, we combine a two-compartment Krogh cylinder model with a toxicity cost function to theoretically optimize the loading of CPA (VMP) in rat kidneys as a model system. First, based on kidney perfusion experiments, we systematically derived the parameters for a CPA transport loading model, including the following: Vb = 86.0% (ra = 3.86 μm), Lp = 1.5 × 10–14 m3/(N·s), ω = 7.0 × 10–13 mol/(N·s), σ = 0.10. Next, we measured the toxicity cost function model parameters as α = 3.12 and β = 9.39 × 10–6. Combining these models, we developed an improved kidney-loading protocol predicted to achieve vitrification while minimizing toxicity. The optimized protocol resulted in shorter exposure (25 min or 18.5% less) than the gold standard kidney-loading protocol for VMP, which had been developed based on decades of empirical practice. After testing both protocols on rat kidneys, we found comparable physical and biological outcomes. While we did not dramatically reduce toxicity, we did reduce the time. As our approach is now validated, it can be used on other organs lacking defined toxicity data to reduce CPA exposure time and provide a rapid path toward developing CPA perfusion protocols for other organs and CPAs.

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Microscopic and Calorimetric Assessment of Freezing Processes in Uterine Fibroid Tumor Tissue

Devireddy

Coad²,

Bischof

2001

Cryobiology

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A Network Thermodynamic Model of Kidney Perfusion

Cited by 3 publications

References 5 publications

Quantitative Measurement and Prediction of Biophysical Response During Freezing in Tissues

Quantitative Measurement and Prediction of Biophysical Response During Freezing in Tissues

Model-Guided Design and Optimization of CPA Perfusion Protocols for Whole Organ Cryopreservation

Microscopic and Calorimetric Assessment of Freezing Processes in Uterine Fibroid Tumor Tissue

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