A new and highly sensitive TLC method to measure hypericin using chemiluminescence

Lotz, Andreas; Habermehl, Peter; Spangenberg, Bernd

doi:10.1080/10826076.2017.1298030

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study, Lotz et al developed a non-validated HPTLC method to determine only HyP by using a mobile system composed of ethyl acetate-water-formic acid-methyl tert-butyl ether-cyclohexane (180:14:14:80:30, v/v/v/v/v) and chemiluminescence for detection. 34 The limit of quantification for HyP was 690 pg. In the other two studies reported by Frommenwiler et al and Booker et al, the authors used qualitative HPTLC techniques to identify adulteration in marketed formulations of H. perforatum.…”

Section: Discussionmentioning

confidence: 99%

“…They reported that the lowest detectable limit for HyF was 100 ng/spot with a good resolution on the plate. In another study, Lotz et al developed a non‐validated HPTLC method to determine only HyP by using a mobile system composed of ethyl acetate‐water‐formic acid‐methyl tert ‐butyl ether‐cyclohexane (180:14:14:80:30, v/v/v/v/v) and chemiluminescence for detection 34 . The limit of quantification for HyP was 690 pg.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of new and validated HPTLC methods for the qualitative and quantitative analysis of hyperforin, hypericin and hyperoside contents in Hypericum species

Saçıcı

Yeşilada

2021

Phytochemical Analysis

View full text Add to dashboard Cite

Hypericum perforatum L. (St. John's wort) is a medicinally important member of Hypericaceae. Many pharmacological activities have been mostly attributed to its hyperforin, hypericin and/or hyperoside contents. Therefore, qualitative and quantitative determinations of these ingredients are essential to justify the beneficial effects of St. John's wort on health. In the European Pharmacopoeia, the TLC and HPLC methods were given for this purpose. High performance thin layer chromatography (HPTLC) has recently become increasingly used as a suitable technique for analysing herbal drugs. This study aims to develop new and validated HPTLC methods to analyse these active components in different Hypericum spp. to find other suitable species to replace the official plant.Methods: Three different mobile phases were developed: n-hexane-ethyl acetate (8:2) for hyperforin analysis, toluene-chloroform-ethyl acetate-formic acid (8:5:3.5:0.6) for hypericin analysis and ethyl acetate-formic acid-acetic acid-water (15:2:2:1) for hyperoside analysis. These newly developed and validated HPTLC systems were further applied to determine their concentrations in different Hypericum species.Results: Hyperforin concentration was found between 6.40 to 26.40 mg/g only in H. triquetrifolium, H. scabrum and two H. perforatum samples; hypericin was detected between 0.81 and 1.41 mg/g only in H. bithynicum, H. perfoliatum, H. triquetrifolium and two H. perforatum samples; and hyperoside was identified in all tested specimens ranging from 1.01 to 9.73 mg/g. The new HPTLC methods developed and validated in the present study may ensure reliable results for the qualification and quantification of hyperforin, hypericin and hyperoside contents in Hypericum species.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of new and validated HPTLC methods for the qualitative and quantitative analysis of hyperforin, hypericin and hyperoside contents in Hypericum species

Saçıcı

Yeşilada

2021

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

A new universal detection system for high-performance thin-layer chromatography plates using chemiluminescence

Spangenberg

2023

JPC-J Planar Chromat

View full text Add to dashboard Cite

High-performance thin-layer chromatography (HPTLC) is a cost-effective method for the separation of small molecules, such as active pharmaceutically ingredients. The method can be used without sophisticated equipment. For example, the detection and quantification of colored compounds on an HPTLC plate can be performed with a CCD camera. For noncolored compounds, a new dipping solution for the generation of chemiluminescence on an HPTLC plates is presented. In combination with a charge-coupled diode (CCD) camera, this can be used as a universal detection system. The efficiency of this new staining reagent is demonstrated by the detection of trimethoprim and sulfamethoxazole from a fixed-dose combination in a ratio of 1:5.

show abstract

Hypericin: A natural anthraquinone as promising therapeutic agent

Zhang

Xia

et al. 2023

Phytomedicine

View full text Add to dashboard Cite

A new and highly sensitive TLC method to measure hypericin using chemiluminescence

Cited by 4 publications

References 15 publications

Development of new and validated HPTLC methods for the qualitative and quantitative analysis of hyperforin, hypericin and hyperoside contents in Hypericum species

Development of new and validated HPTLC methods for the qualitative and quantitative analysis of hyperforin, hypericin and hyperoside contents in Hypericum species

A new universal detection system for high-performance thin-layer chromatography plates using chemiluminescence

Hypericin: A natural anthraquinone as promising therapeutic agent

Contact Info

Product

Resources

About