A New and Rapid Method for the Assay of Bacterial Fatty Acids Using High Resolution Capillary Gas Chromatography and Trimethylsulfonium Hydroxide

Müller, Karl-Dieter; Husmann, H.; Nalik, H. P.

doi:10.1016/s0934-8840(11)80100-3

Cited by 54 publications

(27 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The head group inventory was quantified using a procedure reported by Mangelsdorf et al (37). The fatty acyl side chain inventory was analyzed by gas chromatography (GC)-MS after trans-esterification of the phospholipid fraction to obtain the methylated formerly phospholipid-linked fatty acids using the method described by Mueller et al (41). The position of the double bond(s) in unsaturated phospholipid-linked fatty acids (PLFAs) was determined as described by Pepe et al (50).…”

Section: Shotgun Proteomics By Liquid Chromatography-electrospray Ionmentioning

confidence: 99%

Physiological and Proteomic Adaptation of “Aromatoleum aromaticum” EbN1 to Low Growth Rates in Benzoate-Limited, Anoxic Chemostats

et al. 2012

View full text Add to dashboard Cite

؊1 ) growth rates. A positive correlation to growth rate was observed for cellular parameters (cell size, and DNA and protein contents). The free energy consumed for biomass formation steadily increased with growth rate. In contrast, the energy demand for maintenance increased only from low to med and then remained constant until high . The most comprehensive proteomic changes were observed at low compared to high . Uniformly decreased abundances of protein components of the anaerobic benzoyl coenzyme A (benzoyl-CoA) pathway, central carbon metabolism, and information processing agree with a general deceleration of benzoate metabolism and cellular processes in response to slow growth. In contrast, increased abundances were observed at low for diverse catabolic proteins and components of uptake systems in the absence of the respective substrate (aromatic or aliphatic compounds) and for proteins involved in stress responses. This potential catabolic versatility and stress defense during slow growth may be interpreted as preparation for future needs. Most terrestrial and aquatic habitats are characterized by dynamic fluctuations in the composition and concentration of nutrients, supporting heterotrophic growth of bacteria in a feastand-famine manner. In most cases, poor carbon and nutrient availabilities restrict or even arrest microbial growth (see, e.g., references 19, 27, and 33). Thus, the capacity of bacteria to cope with nutrient limitation is a key determinant for their survival and success in the environment. Substrate limitation and its longknown intimate coupling to bacterial growth rates are best studied by continuous cultivation in chemostats (for an overview, see, e.g., references 20 and 27). Constrained by a defined and constant supply of the limiting nutrient at a specific rate (dilution rate [D]), the bacterial population in a chemostat approaches a steady state characterized by a specific growth rate () and stable growth parameters (39, 45). Combining these quantities with chemostat models can describe the dynamics of bacterial populations at many levels (see, e.g., reference 25).Steady-state responses are best understood in Escherichia coli and other enterobacteria. Early physiological studies revealed a tight coupling of growth rate with the size and molecular composition of cells (7,42,61). Subsequently, growth rate-dependent global alterations in gene expression (21, 30) and proteome signatures (49, 76) were observed. An interesting outcome from these global surveys of slowly growing, carbon-limited cells was the apparently preemptive adaptation to future needs for stress resistance and multiple substrate utilization (for an overview, see reference 19).The denitrifying strain "Aromatoleum aromaticum" EbN1 is a versatile aquatic betaproteobacterium that anaerobically degrades 24 different monoaromatic compounds, including petroleum hydrocarbons (57), phenolic solvents (79), and 3-phenylpropanoids (69). With few exceptions, these aromatic substrates are channeled via specific reaction sequences i...

show abstract

Section: Shotgun Proteomics By Liquid Chromatography-electrospray Ionmentioning

confidence: 99%

Physiological and Proteomic Adaptation of “Aromatoleum aromaticum” EbN1 to Low Growth Rates in Benzoate-Limited, Anoxic Chemostats

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Based on state-of-the-art data, experimental conditions were optimized for the DS determination of LCCE. The optimal stoichiometric ratio of TMSH reagent to the total ester functions has been reported to be 2:1 for the analysis of butter and fatty acids (Schulte and Weber 1989;Muller et al 1990). We first investigated the effect of the quantity of TMSH added to analyze a fully substituted sample, i.e.…”

Section: Optimization Of Reaction Parametersmentioning

confidence: 99%

“…It is accurate and easy to employ with a pre-settled chromatographic analysis. Our endeavor was focused to adapt a known method of lipids characterization (Schulte and Weber 1989;Muller et al 1990). It consists in the transesterification of the esters functions of LCCE with trimethylsulphonium hydroxide (TMSH) using tert-butyl methyl ether (MTBE) as a solvent followed by GC analysis of the transesterified acyl compounds.…”

Section: Introductionmentioning

confidence: 99%

Accurate determination of the degree of substitution of long chain cellulose esters

2008

View full text Add to dashboard Cite

The determination of the degree of substitution (DS) of fatty acid cellulose esters with alkyl chain lengths from C8 to C18 was performed by direct transesterification with trimethylsulphonium hydroxide (TMSH) using tert-butyl methyl ether (MTBE) as a solvent. Transesterification was demonstrated to be quantitative at 75°C in 60 min. The quantification of the formed fatty acid methyl esters was performed by gas chromatography (GC). After the optimization of the method, long chain cellulose esters (LCCE) could be analyzed in a wide range of DS. The obtained values were compared to those given by other existing protocols. LCCE with DS-values in a range of 5 9 10 -5 to 3 were analyzed with high accuracy. Reproducibility is weakened for high DS values if the sample has a compact aspect limiting the accessibility of TMSH to the ester functions. This method can also be suitable for the analysis of mixed cellulose esters.

show abstract

“…Both the free and bound fatty acids were transformed into the methyl esters (FAME) by the direct transesterification method of Muller and colleagues [8] with trimethylsulphonium hydroxide (TMSOH; Macherey-Nagel, Diiren, Germany). The fatty acid profiles were taken according to Miller and colleagues [9] using the capillary column Ultra 2 (crosslinked 5 % Ph Me Silicone; Hewlett-Packard, Palo Alto, USA) and the gas liquid chromatograph model HP 5890 A (Hewlett-Packard).…”

Section: Fa-gc Analysismentioning

confidence: 99%

Application of physico-chemical typing methods for the epidemiological analysis ofSalmonella enteritidisstrains of phage type 25/17

1994

View full text Add to dashboard Cite

SUMMARYEighty-nine Salmonella enteritidis phage type 25/17 strains isolated from a localized outbreak in the German state Nordrhein-Westfalen (outbreak NWI) could not be further differentiated by biochemotyping and plasmid pattern analysis. They were submitted to a complex typing system consisting of modern physico-chemical analytical procedures. In lipopolysaccharide pattern analysis the strains proved to be homogeneous. In multilocus enzyme electrophoresis, outer membrane and whole cell protein pattern (WCPP) analysis, and Fouriertransform infrared (FT-IR) spectroscopy (increasing extent of differentiation in the given order) strains deviating from each basal pattern were found. The extent of correspondence in these deviations was satisfactory.Forty-six strains of the same sero-and phage type, however, obtained from different outbreaks, were additionally typed. The results obtained with them indicate that the data of the first group were not restricted to strains from outbreak NWI, but of general validity.It was found that both WCPP and FT-IR represent valuable methods for the sub-grouping of bacteria.

show abstract

A New and Rapid Method for the Assay of Bacterial Fatty Acids Using High Resolution Capillary Gas Chromatography and Trimethylsulfonium Hydroxide

Cited by 54 publications

References 14 publications

Physiological and Proteomic Adaptation of “Aromatoleum aromaticum” EbN1 to Low Growth Rates in Benzoate-Limited, Anoxic Chemostats

Physiological and Proteomic Adaptation of “Aromatoleum aromaticum” EbN1 to Low Growth Rates in Benzoate-Limited, Anoxic Chemostats

Accurate determination of the degree of substitution of long chain cellulose esters

Application of physico-chemical typing methods for the epidemiological analysis ofSalmonella enteritidisstrains of phage type 25/17

Contact Info

Product

Resources

About