A new assay for cholesterol and cholesterol esters in serum which is not affected by bilirubin

Babson, Arthur L.; Shapiro, Paul O.; Phillips, George E.

doi:10.1016/0009-8981(62)90062-1

Cited by 91 publications

(13 citation statements)

References 13 publications

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“…(2) L'étude analytique et longitudinale de ces hyperlipémies, ainsi que l'enquête géné tique biologique ont utilisé les techniques courantes de dosage du cholestérol total par une adaptation de la technique de Babson et al [3], des triglycérides par la technique de Van H andel et Z ilversmit [28], des lipides totaux [5], des phospholipides [31], ainsi que pour la caractérisation des lipoprotéines, l'électrophorèse sur papier des lipoprotéines de Lees et Hatch [21 ], et le test de décantation de G ordis [ 15] en gradient de polyvinylpyrrolidone. Les études de sensibilité diététique ont été réalisées sous la supervision de notre départe ment diététique pour la composition des régimes prescrits, la surveillance de leur mode d'application et les interviews diététiques de contrôle.…”

Section: Matériel Et Méthodes ( !) Critères De Sélectionunclassified

Hyperlipémies dépendantes de l’alcool

Gennes¹,

Thomopoulos²,

Truffert³

et al. 1972

Ann Nutr Metab

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The authors report a study of 28 cases of hyperlipemia due to alcohol. Clinically, they emphasize the predominance of the male sex, the high incidence of digestive disturbances, weight loss and hepatomegaly and the rarity of cardiovascular involvement. From the biochemical point of view, the majority of cases present an endogenous hyperlipemia of the same type as that induced by carbohydrates. Attention is also drawn to the disturbed glucose balance and the frequent hyperuricemia. Moreover, demonstration of lipid anomalies in the relatives of 11 cases out of 15 suggests a genetic predisposition rather analogous to that in carbohydrate hyperlipemia. Treatment by simple withdrawal of alcohol is always effective, but very unreliably adhered to by these patients.

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Section: Matériel Et Méthodes ( !) Critères De Sélectionunclassified

Hyperlipémies dépendantes de l’alcool

Gennes¹,

Thomopoulos²,

Truffert³

et al. 1972

Ann Nutr Metab

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“…Blood specimens obtained from the survivors of both groups were analyzed for serum levels of total cholesterol, phospholipids, and triglycerides by the following methods: total cholesterol extracted with ethyl acetate, using AI(OH), for removal of bilirubin and ferric chloride for color reaction [1]; phospholipids extracted with 2 : I chloroform-methanol, digested by perchloric acid and reacted with ammonium molybdate for color development (201; triglycerides extracted with chloroform, using zeolite for removal of phospholipids, and reacted with chromotropic acid for color development 1171.…”

Section: Methodsmentioning

confidence: 99%

Production of Atherosclerosis in Lathyritic Aortas of Rats

Hosoda¹,

Suzuki²

1970

Pathobiology

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Forty weanling male rats were administered 0.4% beta-aminopropionitrile fumarate (BAPN) for 3 weeks and were then fed an atherogenic diet supplemented with 0.2% BAPN for 4 weeks and with 0.l% BAPN for 9 weeks. Twenty control rats were fed the atherogenic diet without added BAPN. Grossly, intimal lipidosis of the aorta stained with Sudan IV appeared more extensive in test than in control rats. Histologically, intimal lipidosis of the aorta in test rats appeared as early as at 3 weeks on the atherogenic diet. Six test rats showed medial dissection of the thoracic aorta with intimal lipidosis. Dissected spaces of the aorta were filled with proliferating medial cells and, in the subendothelial zone, the smooth muscle cells were mixed with lipid-laden macrophages. Electron microscopically, the aortic intima of test rats showed lipid-laden macrophages and smooth muscle cells in widened subendothelial space. Between the test and control groups, none of the major serum lipid fractions showed significant difference.The results suggest that lathyritic changes of rat aortas induce not only active repair of damaged media but also enhance intimal lipid deposition.

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“…The objective of this experiment was to characterize nutritional ketosis by changes in levels of blood metabolites due to a gradual decrease in intake of dietary energy, and to quantify the degree of energy depletion in terms (26), p-hydroxybutyrate (27), glucose (22), total esters (24), cholesterol (2), phospholipid phosphorus (4) and free fatty acids ( 1). The data from the experiment were subjected to least squares analysis and the rneans tested by the multiple range test (7 (14).…”

Section: Introductionmentioning

confidence: 99%