A new biological glue from gelatin and poly (L-glutamic acid)

Otani, Yuto; Tabata, Yasuhiko; Ikada, Yoshito

doi:10.1002/(sici)1097-4636(199606)31:2<157::aid-jbm2>3.0.co;2-m

Cited by 107 publications

(60 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The addition of γ‐PGA into the formulation could accelerate the gel formation rate, increase the viscosity, and improve antibacterial activity because of its abundant carboxyl groups, γ‐peptide bonds, and helical structure. However, as shown in Figure 3, the viscosity of the solution mixture slowly increases with time without showing a sharply increasing peak, in contrast to previous studies 28, 30. In this study, we reconstituted collagen molecules in γ‐PGA solution first, and then crosslinked them with EDC solution.…”

Section: Discussionmentioning

confidence: 68%

“…In this study, we quantified the adhesive properties according to ASTM F2255‐05 regulations to obtain compatible mechanical properties. The shear strength of the collagen/γ‐PGA cohesive plug gel was significant higher than that of fibrin glue, and the strength continually increased with time over 24 h. Despite the different test methods, the results of the shear strength of the collagen/γ‐PGA cohesive plug gel is significant higher than previous studies 16, 18, 30. The high shear strength might be due to collagen molecules assembled into larger fibers with D‐period structure.…”

Section: Discussionmentioning

confidence: 69%

See 1 more Smart Citation

Fabrication and evaluation of a biodegradable cohesive plug based on reconstituted collagen/γ‐polyglutamic acid

et al. 2010

View full text Add to dashboard Cite

In the past decade, numerous studies have been devoted to developing natural bioadhesives that have the notable capacity to adhere to wet surfaces. Collagen and γ-polyglutamic acid (γ-PGA) are well-known natural hydrophilic polymers that have both been utilized for their versatility in a wide range of biomedical applications. The aim of this study was the construction and characterization of a cohesive plug composed of γ-PGA and reconstituted collagen fibrils crosslinked with water-soluble carbodiimide. Transmission electron microscopy examinations confirmed that the collagen fibrils in the reconstituted collagen/γ-PGA gel retained their native specific D-period structure. This unique D-pattern structure of collagen plays a major role in hemostasis and is also related to several cellular behaviors. The bonding strength of the reconstituted collagen/γ-PGA adhesive was approximately 42.9 ± 4.0 KPa after 5 min of application and increased to 76.5 ± 15.1 KPa after 24 h. This was much stronger than the fibrin adhesive, whose bonding strength was 30.9 ± 0.2 KPa. Furthermore, the reconstituted collagen/γ-PGA gel degraded gradually after subcutaneous implantation in the backs of rats over a period of 8 weeks, without any severe inflammatory response. On the basis of the histological analysis, fibroblasts migrated into the gel while it degraded, which indicates that the gel is not harmful to cellular activity. Together, these findings demonstrate that using reconstituted collagen with retained D-periodicity as a component of the bioadhesive is a possibly better option to formulate effective adhesiveness and is promising as a scaffold for tissue repair.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 69%

Fabrication and evaluation of a biodegradable cohesive plug based on reconstituted collagen/γ‐polyglutamic acid

et al. 2010

View full text Add to dashboard Cite

show abstract

“…This gel gives higher bonding strength to various soft tissues than that of clinically used fibrin glues [72,73]. Degradation of the gel did not cause any significant inflammation in the animal model.…”

Section: Gelatin-based Gluementioning

confidence: 86%

“…One of them is a rapidly curable bioabsorbable glue consisting of gelatin and poly(Lglutamic acid) (PLGA) [72,73]. Mixing of gelatin and PLGA aqueous solutions with water-soluble carbodiimide (WSC) forms a hydrogel within a few seconds through amide formation between amino groups of gelatin and carboxyl groups of PLGA, as shown in Fig.…”

Section: Gelatin-based Gluementioning

confidence: 99%

Adhesion of Cells and Tissues to Bioabsorbable Polymeric Materials: Scaffolds, Surgical Tissue Adhesives and Anti-adhesive Materials

Suzuki

Ikada

2010

Journal of Adhesion Science and Technology

View full text Add to dashboard Cite

Interaction between the body and implanted materials is a very complex phenomenon, and understanding the concept of cell and tissue adhesion, which is the first event that occurs when a biomaterial is implanted, is of great importance. Currently, bioabsorbable materials are preferably used as biomaterials. They are only present in the body temporary, and promote guided cell and tissue growths for the required time. Hence, the choice of the material, depending on the application, becomes crucial. This article offers an overview of cell and tissue adhesion to three types of clinically-important bioabsorbable materials from a materials perspective: scaffolds, surgical tissue adhesives, and anti-adhesive materials.

show abstract

“…Gelatin-based sealants have been created using different crosslinking agents, such as genipin [18], water-soluble carbodiimides [18,19], transglutaminase [20,21], biopolymers [22][23][24] and glutaraldehyde [25]. The use of chemical crosslinking agents has a risk of cytotoxicity caused by the unreacted compounds and their degradation products.…”

Section: Surgical Hemostatic Agentsmentioning

confidence: 99%

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Yamashita

Tabayashi

et al. 2015

Bio-Medical Materials and Engineering

View full text Add to dashboard Cite

BACKGROUND: The bilayer gelatin sealing sheet was developed as a safe, effective, easy-to-handle and low-cost hemostatic agent. OBJECTIVE: To examine the feasibility of gelatin sealing sheets using a canine arterial hemorrhage model. METHODS:In vivo degradation of gelatin sealing sheets was examined by implanting subcutaneously in rats. For the hemostatic and anti-adhesion efficacy investigations, femoral arteries of dogs were pricked with syringe needle to make a small hole and a gelatin (i.e. experimental group) or fibrin glue sealing sheet (i.e. control group) was applied on the hole to stop bleeding (n = 8). After discontinuation of the bleeding, the skin incisions were closed and re-examined 4 weeks postoperatively. RESULTS: From the degradation study, 4 h thermally treated gelatin sheet which degraded within 3 weeks in vivo was chosen for the further hemostatic study. In all cases of gelatin and fibrin glue sealing sheets, bleeding from the needle hole on canine femoral arteries was effectively stopped. Postoperative adhesions and inflammation at the site in the experimental group were significantly less than those in the control group (P < 0.01 for adhesion scores). CONCLUSIONS: The gelatin sealing sheet was found to be as effective as the fibrin glue sealing sheet as a surgical hemostatic agent, and more effective in preventing postoperative adhesions.

show abstract

A new biological glue from gelatin and poly (L-glutamic acid)

Cited by 107 publications

References 21 publications

Fabrication and evaluation of a biodegradable cohesive plug based on reconstituted collagen/γ‐polyglutamic acid

Fabrication and evaluation of a biodegradable cohesive plug based on reconstituted collagen/γ‐polyglutamic acid

Adhesion of Cells and Tissues to Bioabsorbable Polymeric Materials: Scaffolds, Surgical Tissue Adhesives and Anti-adhesive Materials

Gelatin sealing sheet for arterial hemostasis and anti-adhesion in vascular surgery: A dog model study

Contact Info

Product

Resources

About