A New Colorimetric Sensor for Cysteine Determination Based on Dual Reacting‐mediated Strategy

Abstract

Rapid and simple determination of cysteine is a key requirement for both diagnosis and treatment of diseases. A novel concept is proposed for combining dual reacting-mediated process into the construction of a simple yet effective colorimetric strategy for cysteine detecting, which is based on the analyte-induced formation of ferrous ion and graphene oxide-supported ionic recognition through selective coordination process between phenanthroline molecule and ferrous ion. In colorimetric analysis, the simple principle of ferrous ion that cysteine-induced can be recognized by phenanthroline indicator due to their good binding affinity and therefore provided a sensitive optical readout for conveniently detecting cysteine. Proofconcept experiments demonstrate that the proposed colorimetric system shows rapid response toward cysteine determination as well as with a satisfying detection limit of 4.8 μM only by using simple UV-Vis spectroscopy and 15 μM with the naked eye.

Designing an “Off–On” Fluorescence Sensor Based on Cluster-Based CaII-Metal–Organic Frameworks for Detection of l-Cysteine in Biological Fluids

“…The relative standard deviation was found to be 6.6% by operating three repeated tests of 20 μM l -cysteine (Table S3, Supporting Information). Moreover, the fluorescence sensor possessed a relatively wide linear range and a low detection limit, which was compared to or even better than the characteristic quantities of the other reported methods as shown in Table . − ,,− …”

“…22−25 Thus, the development of fluorescence sensors for L-cysteine determination has become quite desirable in diagnosis of some diseases. Currently, some classical analytical techniques have already been applied for quantitative detection of L-cysteine, such as colorimetric methods, 26 electrochemical methods, 27 highperformance liquid chromatography, 28 mass spectrometry, 29 capillary electrophoresis, 30 and fluorescence analysis methods. 31 Nevertheless, these techniques still have many shortcomings and limitations.…”

A novel Salen-based dual channel sensor for easy and selective nanomolar detection of l-cysteine

“…While most of these techniques furnish both quantitative and qualitative insights into the presence of L-cysteine in physiological samples, they are not without their inherent limitations and drawbacks. [19][20][21][22] In contrast to existing methodologies, colorimetric analysis stands out as a straightforward and userfriendly approach for analyte detection. 23,24 Nevertheless, it faces notable limitations, primarily in terms of accuracy and susceptibility to interference.…”