A new, easy-to-make pectin-honey hydrogel enhances wound healing in rats

Abstract

BackgroundHoney, alone or in combination, has been used for wound healing since ancient times and has reemerged as a topic of interest in the last decade. Pectin has recently been investigated for its use in various biomedical applications such as drug delivery, skin protection, and scaffolding for cells. The aim of the present study was to develop and evaluate a pectin-honey hydrogel (PHH) as a wound healing membrane and to compare this dressing to liquid honey.MethodsThirty-six adult male Sprague-Dawley rats were anesthetized and a 2 × 2 cm excisional wound was created on the dorsum. Animals were randomly assigned to four groups (PHH, LH, Pec, and C): in the PHH group, the pectin-honey hydrogel was applied under a bandage on the wound; in the LH group, liquid Manuka honey was applied; in the Pec group, pectin hydrogel was applied (Pec); and in the C group, only bandage was applied to the wound. Images of the wound were taken at defined time points, and the wound area reduction rate was calculated and compared between groups.ResultsThe wound area reduction rate was faster in the PHH, LH, and Pec groups compared to the control group and was significantly faster in the PHH group. Surprisingly, the Pec group exhibited faster wound healing than the LH group, but this effect was not statistically significant.ConclusionThis is the first study using pectin in combination with honey to produce biomedical hydrogels for wound treatment. The results indicate that the use of PHH is effective for promoting and accelerating wound healing.

Theophylline‐loaded pectin‐based hydrogels. II. Effect of concentration of initial pectin solution, crosslinker type and cation concentration of external solution on drug release profile

“…5,[44][45][46][47][48] It is widely used as a thickener, gelling agent, emulsifier, and so forth in commercial applications. 49 Since pectin and pectin-based materials in 3D structure are also extensively used in biomedical applications, 14,50,51 there are some scientific publications investigated the effect of structure and source of pectin on rheological behavior of pectin gels. Chemical structure such as molecular weight, degree of esterification, and source of pectin are also important parameters.…”

“…Most of the studies are about the relationship between chemical properties of pectin and rheological properties of pectin solution 5,45,49 or between pectin concentration of the solution and its rheological properties. 49 Since pectin and pectin-based materials in 3D structure are also extensively used in biomedical applications, 14,50,51 there are some scientific publications investigated the effect of structure and source of pectin on rheological behavior of pectin gels. 15,52 We also determined the viscoelastic properties of the 3D pectin hydrogels prepared in three different pectin solutions without drug, P2CC7-B, P4CC7-B, and P6CC7-B.…”

Stimulation of wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF)

“…Pectin is a heterosaccharide found in plant cell walls. 22 It is very cheap, can be extracted from renewable sources, and has biocompatible properties. It has been investigated for many different biomedical applications, such as drug delivery, tissue engineering, and wound dressing.…”

Keratin scaffolds with human adipose stem cells: Physical and biological effects toward wound healing

“…Keratin is a natural material with excellent biocompatibility and has been evaluated (Batzer, Marsh, & Kirsner, ; Bhardwaj et al, ; Rouse & Van Dyke, ). Regarding to the common biomaterials for wound healing, there are various dressings or gels used for physical trauma, chronic wound, and other types of injuries, which include hydrocolloids (Grange‐Prunier, Couilliet, Grange, & Guillaume, ; Koo, Piletta‐Zanin, Politta‐Sanchez, Milingou, & Saurat, ), alginates (Balakrishnan, Mohanty, Umashankar, & Jayakrishnan, ; Wang et al, ), hydrogels (Kamoun, Kenawy, & Chen, ; Madaghiele, Demitri, Sannino, & Demitri, ), polyurethane (Gultekin et al, ; Yücedag et al, ), chitosan (Liang et al, ; Ribeiro et al, ; Stricker‐Krongrad et al, ), gelatin (Tanaka, Nagate, & Matsuda, ; Ulubayram, Nur Cakar, Korkusuz, Ertan, & Hasirci, ) pectin (Giusto et al, ; Tummalapalli et al, ), and hyaluronic acid (Neuman, Nanau, Oruna‐Sanchez, & Coto, ; Price, Myers, Leigh, & Navsaria, ). However, these biomaterials have limited application due to poor in vivo persistency.…”