A new ethanol biosensor based on polyfluorene-g-poly(ethylene glycol) and multiwalled carbon nanotubes

Abstract

Functionalization of the conjugated polymers has gained considerable interest in the biomedical engineering and biosensing applications as distinct properties can be imparted to the corresponding pristine analogues. In the present work, we report a novel sensing platform for the quantification of ethanol through a macromolecular design involving polyfluorene-g-poly(ethylene glycol) (PF-g-PEG) and multiwalled carbon nanotubes (MWCNTs). First, poly(ethylene glycol) with fluorene functionality (PEG-FL) was synthesized with a one-step procedure and characterized. The nanotube modified electrodes were then used as working electrodes for the electropolymerization of PEG-FL macromonomer to form PF-g-PEG films on the electrode surface. Finally, alcohol oxidase enzyme was immobilized on the modified surfaces. Similar devices without MWCNTs or PF-g-PEG were prepared and compared. Sensor properties for selective ethanol detection were investigated and it was found that PF-g-PEG modified MWCNTs exhibited the highest sensing ability. The potential practical application of the fabricated biosensor is demonstrated in alcoholic drinks for the analysis of ethanol contents.

Development of Electrochemical HRP-MWCNT-Based Screen-Printed Biosensor for the Determination of Phenolic Compounds in Effluent from Washing Coffee Beans

“…Because the CNTs have electrochemical stability and high surface area, they are very employed in sensors and biosensors. 15,16 Screen printed electrodes (SPEs) have also been widely employed in sensors for their especially low cost, fast response and large-scale production. 17 Regardless of these advantages, signal conductivity remains a weak point of SPE electrode.…”

Carbon nanotubes in biomedical applications: current status, promises, and challenges

“…The cathodic peak current showed a good linear response to the fraction of MDR with a correlation coefficient of 0.995 was observed for the cathodic peak current indicating an easy prediction of the MDR fraction based on the calibration curve of the cathodic peak current versus the fraction of MDR [ 72 ]. A sensor-based on polyfluorene-g-poly(ethylene glycol) (PF-g-PEG) and MWCNTs was developed for the quantification of ethanol in alcoholic drinks by Bekmezci et al, with high sensitivity [ 73 ]. Ghrera et al fabricated an impedimetric microfluidic-based biosensor for quantification of the DNA sequence specific to chronic myelogenous leukemia (CML).…”

Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications

“…There have been several studies on how enzyme‐incorporated nano‐biosensors have been found promising. For example, alcohol dehydrogenase/diamond NPs on phenothiazine support matrix (Revenga‐Parra et al, 2020), screen‐printed electrodes modified with GNPs and MWCNTs/polyneutral red film/alcohol dehydrogenase (Bilgi & Ayranci, 2016), polyfluorene‐g‐polyethylene glycol/MWCNTs/alcohol oxidase (Bekmezci et al, 2020) and the immobilization of alcohol dehydrogenase on Fe 3 O 4 @Au NPs (Samphao et al, 2015). Table 6 shows the summary of the enzyme‐incorporated biosensors using nanomaterials.…”