A new high performance liquid chromatographic method for quantification of atomoxetine in human plasma and its application for pharmacokinetic study

Determination of atomoxetine in human plasma by a high performance liquid chromatographic method with ultraviolet detection using liquid–liquid extraction

“…The detection of atomoxetine at 210 nm provided maximum intensity with minimum interference. Both solid phase extraction (SPE) and liquid-liquid extraction (LLE) sample preparation methods have been reported [15,[18][19][20]. The SPE method used polystyrenedivinylbenzene to extract atomoxetine from plasma and urine [18].…”

“…So far, the reported analytical methods for atomoxetine include GC [15], LC-MS [18] HPLC-FL [19], and HPLC-UV [20]. The GC method reported by Farid et al [15] for the quantification of atomoxetine in human plasma and urine used liquid-liquid extraction (LLE) and electron-capture detection with a structural analogue as internal standard.…”

“…However, derivatization with the reagent 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride (DIB-Cl) during sample pretreatment is time-consuming and complex, thereby limiting applicability to routine clinical analysis. When the manuscript was under revising, Patel et al [20] reported a HPLC-UV method using liquid-liquid extraction with tertiary butyl methyl ether. The lower limit of atomoxetine quantification was 50 ng/mL with the detection wavelength at 272 nm.…”

Validated Stability-Indicating UPLC and Derivative Synchronous Fluorescence Spectroscopy Methods for the Determination of Atomoxetine Hydrochloride in Pharmaceutical Preparation

“…These procedures include spectrophotometry and/or fluorimetry [3][4][5][6], high performance thin layer chromatography [7], voltammetry [8], HPLC [9][10][11], and LC/MS Abstract .HCl [12,13]. Forced degradation study was not reported in these articles [4][5][6][7][8][9][10][11][12][13]; in addition, a paper on the stability indicating HPLC assay of ATM in presence of unknown degradation products was developed [14]. However, the paper did not deals neither with the determination of ATM in spiked plasma nor with analysis of the drug in presence of known degradates using (DSF) derivative synchronous fluorometry.…”

Determination of atomoxetine metabolites in human plasma by liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study

“…Several analytical methods for the determination of atomoxetine using liquid chromatography coupled with ultraviolet detection [7,8], fluorescence [9], or tandem mass spectrometry (LC-MS/MS) [10,11] have been reported. These methods are useful for the pharmacokinetic study of atomoxetine.…”