Determination of atomoxetine in human plasma by a high performance liquid chromatographic method with ultraviolet detection using liquid–liquid extraction

Guo, Wei; Li, Wenbiao; Guo, Gui-Xin; Zhang, Jun; Zhou, Bei-Lei; Zhai, Yuanzheng; Wang, Chuanyue

doi:10.1016/j.jchromb.2007.04.007

Cited by 28 publications

(25 citation statements)

References 19 publications

(31 reference statements)

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“…[2,6,7] Initially, the mobile phase without phosphate buffer was used and the retention time of ATO was increased to 25 min with poor peak morphology originating from the lack of pH optimization. Then, the mobile phase including phosphate buffer at different pH values (in the pH range of 2.5-5.5) was investigated for the chromatographic separation of ATO.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

“…It has been marked by symptoms of inattention, hyperactivity, and impulsiveness that impair academic and social functioning. [1,2] ATO is predominantly metabolized by cytochrome P450 (CYP) 2D6 enzyme. It has been reported that maximum plasma concentrations of ATO have been observed in the range of 160-184 ng=mL in CYP2D6 extensive metabolizers and up to 925 ng=mL in genetically deficient CYP2D6 poor metabolizers after oral administration of 20 mg twice daily in adults.…”

Section: Introductionmentioning

confidence: 99%

“…Several chromatographic methods have been reported for the determination of ATO in human plasma including, high performance liquid chromatography (HPLC) [1][2][3] and liquid chromatography-tandem mass spectrometry (LC=MS=MS). [4] Also, chiral separation of ATO enantiomers is described.…”

Section: Introductionmentioning

confidence: 99%

“…[9] These methods developed for the analysis of ATO in its dosage forms and also the chiral method, did not offer the adequate sensitivity for measuring ATO in plasma. [5][6][7][8][9] The methods reported for the determination of ATO in plasma offered adequate sensitivity; however, they involved liquid-liquid [1][2][3] or solid phase [4] extraction of ATO from the plasma samples prior to analysis. These time-consuming and expensive extraction procedures increase the overall analysis time and the cost of the analysis.…”

Section: Introductionmentioning

confidence: 99%

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A Simple and Specific HPLC Method for the Determination of Atomoxetine in Pharmaceuticals and Human Plasma

Doğrukol-Ak

Yeniceli

2010

Journal of Liquid Chromatography & Related Technologies

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Turkey& A liquid chromatographic method has been described for the determination of atomoxetine in pharmaceuticals and human plasma. Plasma samples were analyzed after a simple, one step protein precipitation with methanol, and chromatographic separation of atomoxetine and carbamazepine (internal standard) was carried out using the optimum mobile phase of a methanol= acetonitrile=phosphate buffer (10 mM, pH 3.0) (35:15:50, v=v=v). Limit of quantification values were 45.2 and 49.5 ng=mL for atomoxetine in the mobile phase and human plasma, respectively. Fully validated method is reproducible and selective for the determination of atomoxetine in pharmaceuticals and human plasma.

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Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Simple and Specific HPLC Method for the Determination of Atomoxetine in Pharmaceuticals and Human Plasma

Doğrukol-Ak

Yeniceli

2010

Journal of Liquid Chromatography & Related Technologies

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“…Several analytical methods for the determination of atomoxetine using liquid chromatography coupled with ultraviolet detection [7,8], fluorescence [9], or tandem mass spectrometry (LC-MS/MS) [10,11] have been reported. These methods are useful for the pharmacokinetic study of atomoxetine.…”

Section: Introductionmentioning

confidence: 99%

Determination of atomoxetine metabolites in human plasma by liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study

Choi

Bae

Lee

et al. 2012

Journal of Chromatography B

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A liquid chromatography/tandem mass spectrometry assay for the analysis of atomoxetine in human plasma and in vitro cellular samples

Appel

Brinda

Markowitz

et al. 2012

Biomedical Chromatography

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A simple, rapid and sensitive method for quantification of atomoxetine by liquid chromatography- tandem mass spectrometry (LC-MS/MS) was developed. This assay represents the first LC-MS/MS quantification method for atomoxetine utilizing electrospray ionization. Deuterated atomoxetine (d3-atomoxetine) was adopted as the internal standard. Direct protein precipitation was utilized for sample preparation. This method was validated for both human plasma and in vitro cellular samples. The lower limit of quantification was 3 ng/ml and 10 nM for human plasma and cellular samples, respectively. The calibration curves were linear within the ranges of 3 ng/ml to 900 ng/ml and 10 nM to 10 μM for human plasma and cellular samples, respectively (r2 > 0.999). The intra- and inter-day assay accuracy and precision were evaluated using quality control samples at 3 different concentrations in both human plasma and cellular lysate. Sample run stability, assay selectivity, matrix effect, and recovery were also successfully demonstrated. The present assay is superior to previously published LC-MS and LC-MS/MS methods in terms of sensitivity or the simplicity of sample preparation. This assay is applicable to the analysis of atomoxetine in both human plasma and in vitro cellular samples.

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Determination of atomoxetine in human plasma by a high performance liquid chromatographic method with ultraviolet detection using liquid–liquid extraction

Cited by 28 publications

References 19 publications

A Simple and Specific HPLC Method for the Determination of Atomoxetine in Pharmaceuticals and Human Plasma

A Simple and Specific HPLC Method for the Determination of Atomoxetine in Pharmaceuticals and Human Plasma

Determination of atomoxetine metabolites in human plasma by liquid chromatography/tandem mass spectrometry and its application to a pharmacokinetic study

A liquid chromatography/tandem mass spectrometry assay for the analysis of atomoxetine in human plasma and in vitro cellular samples

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