A new method for anti‐negative interference of calcium dobesilate in serum creatinine enzymatic analysis

Abstract

Background Serum creatinine is a widely used biomarker for evaluating renal function. Sarcosine oxidase enzymatic (SOE) analysis is currently the most widely used method for the detection of creatinine. This method was negatively interfered with by calcium dobesilate, causing pseudo‐reduced results. The aim of this study was to explore a new method to alleviate the negative interference of this drug on creatinine detection. Method We formulated eight drug concentrations and 12 creatinine concentrations from serum. The SOE method, the new method, and the Jaffe method were used for detection in five systems. Creatinine biases were analyzed under the conditions with or without the interference of calcium dobesilate, at consistent or inconsistent creatinine concentrations. Creatinine concentrations were also analyzed at three medical decision levels (MDLs). Results Calcium dobesilate had negative interference in creatinine SOE analysis. With the increase in calcium dobesilate concentrations, the negative bias increases. The new BG method showed an anti‐negative interference effect. In the Roche system, the BG method reduced the negative bias from −71.11% to −16.7%. In the Abbott system, bias was reduced from −45.15% to −2.74%. In the Beckman system, the bias was reduced from −65.36% to −7.58%. In the Siemens system, the bias was reduced from −58.62% to −7.58%. In the Mindray system, the bias was reduced from −36.29% to −6.84%. Conclusion The new method alleviated the negative interference of calcium dobesilate in creatinine SOE detection. The negative bias could be reduced from −60% or −70% to less than −20%.

Emergency medicine pharmacotherapy compromises accuracy of plasma creatinine determination by enzyme-based methods: real-world clinical evidence and implications for clinical practice

“…During the past two decades, Jaffé methods have been widely replaced by enzyme-based colorimetric methods employing sarcosine oxidase/peroxidase reaction chemistry ( 2 ). It has been shown repeatedly that enzymatic methods are susceptible to negative interference caused by several drugs, such as etamsylate ( 3 ), metamizole ( 4 , 5 ), paracetamol, N-acetyl-p-benzoquinone imine (NAPQUI) ( 5 ), acetylcysteine ( 6 ), derivatives of salicylic acid ( 5 , 7 ), high-dose acidum ascorbicum ( 8 ), and dobesilate ( 9 ). Negative interference by catecholamines at clinically relevant concentrations was also shown to affect these methods ( 10 ), and recently, monoclonal immunoglobulins have also been reported to interfere ( 11 , 12 ).…”