A new method for isolation of polyethylene wear debris from tissue and synovial fluid

Visentin, M.; Stea, Susanna; Squarzoni, Stefano; Antonietti, B; Reggiani, Matteo; Toni, Aldo

doi:10.1016/j.biomaterials.2004.01.001

Cited by 39 publications

(27 citation statements)

References 22 publications

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“…Centrifugation at 105,000 × g is generally used for separation of cell organelles without any significant damage to them [15]. However, when such a possibility was suggested for polyethylene wear particles [9], it was desirable to investigate the problem and verify the correctness of both our own [7] and other isolation techniques [10][11][12], which all employ centrifugation. There are two theoretical possibilities how centrifugation could have influenced wear particle morphology: (i) elongated particles might have been changed to spherical due to mutual collisions and collisions with vessel edges at very high centrifugation speeds and (ii) very small particles might have been isolated in higher amounts at higher centrifugation rates.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the methods rely on tissue digestion, washing, filtration and/or centrifugation [7,[10][11][12]. In one of the studies [9] it was suggested that ultracentrifugation or centrifugation may change particle morphology. However, this assumption has been presented without any proof.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, many authors paid attention to the isolation, purification and characterization of in vivo, "native", biologically active UHMWPE wear particles [6,7] from periprosthetic tissues. The existing isolation methods are being improved [7,8] and new methods appear [9]. Most of the methods rely on tissue digestion, washing, filtration and/or centrifugation [7,[10][11][12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Can centrifugation affect the morphology of polyethylene wear debris?

Zolotarevova

Fejfarkova

Entlicher

et al. 2008

Wear

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Can centrifugation affect the morphology of polyethylene wear debris?

Zolotarevova

Fejfarkova

Entlicher

et al. 2008

Wear

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“…Quantification was defined by 20 fields capturing 90 micron 2 each, representing all the regions of the filter. The particles were counted, their major diameter measured and their chemical composition was verified by energy dispersive X-ray microanalysis; analysis was performed at 25-mm WD with an Oxford INCA Energy 200 apparatus [20]. The amount and the chemical composition of metal particles were then scored for each sample by two separate operators.…”

Section: Detection Of Particles In Synovial Fluidmentioning

confidence: 99%

Metal-on-metal hip prostheses: Correlation between debris in the synovial fluid and levels of cobalt and chromium ions in the bloodstream

Pasquale

Stea

Squarzoni

et al. 2013

International Orthopaedics (SICOT)

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Purpose Hip prostheses with metal-on-metal (MoM) coupling can release cobalt-chromium particles and ions. The aim of this work is to verify the correlation between particles in the synovial fluid and circulating ions. Methods Forty patients were enrolled; particles from synovial fluid were analysed by SEM-EDX (Scanning Electron Microscopy-Energy Dispersion X-rays analysis) and levels of circulating Co and Cr were assayed by ICP-MS (inductively-coupled plasma mass spectrometry). Results In 16 cases we did not find any particles in the synovial fluid and the Co level in whole blood was 0.05-4.42 ppb; in seven with few particles the blood level was 2.2-15.6 ppb; in six cases with several particles the level was 5.0-54.3 ppb; finally, in 11 cases we isolated not only Co-Cr particles, but also Cr particles with low or absent Co and in these patients the circulating level of Co was 23.8-109.6 ppb. Co in serum and Cr level both whole blood and serum have shown a similar trend to Co; the correlation between all these values and the corresponding particles is statistically significant in all cases. Conclusion Co and Cr both in serum and whole blood represents a systemic representation of the particle release at local level and can therefore be used to confirm a diagnosis and monitor the wear process of MoM articular prostheses.

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“…From the size distributions shown in Table 1, the UHMWPE debris from the spinal simulator was somewhat in agreement with that of other TJRs [9,[15][16][17][18][19][20][21]. However it is difficult to make direct comparisons as test, sample preparation and assessment methodologies and equipment vary between research groups [8].…”

Section: Discussionmentioning

confidence: 82%

The evolution of polymer wear debris from total disc arthroplasty

2015

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a b s t r a c tTotal disc arthroplasty is an alternative to spinal fusion, aimed at preserving flexibility; these devices typically involve a cobalt chrome molybdenum alloy socket articulating against an ultra-high molecular weight polyethylene (UHMWPE) ball. As with all artificial joints, wear debris is of particular concern due to its effect on both implant life and the in vivo biological reactions that can occur. In this paper, a profile of the UHMWPE wear debris generated from disc arthroplasty, tested on a spine simulator, is built with a combination of SEM image analysis tools. SEM images were analysed by computer vision, which allowed size and shape information to be extracted and images to be categorised by the shared topological features on individual wear particles. The computer visions techniques were based on a Scale Invariant Feature Transform (SIFT) to extract key point data from individual images and a Support Vector Decision Machine (SVM) to filter images based on a series of trained parameters. As certain wear particle morphology is predominantly produced by a particular wear regime, grouping wear particles by morphology and size made it possible to infer the relative rates of various wear regimes responsible for wear debris generation. By sampling synovial lubricant at intervals throughout the tribological test, the predominant wear regimes and particle sizes were tracked over the course of the implant life. Wear debris samples were taken at 12 intervals over a 5 million cycle test. The majority of debris was found to be 0.88 μm in equivalent circle diameter, with an aspect ratio (defined as the major over the minor diameter of the smallest possible encompassing ellipse of the debris) of 1.55. There was a decreasing trend in average particle size as the number of cycles increased. During the early stages of the test, adhesion and abrasion were dominant in forming particle morphologies, however after 2 million cycles; particles generated as a result of fatigue became the major particle morphology.

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A new method for isolation of polyethylene wear debris from tissue and synovial fluid

Cited by 39 publications

References 22 publications

Can centrifugation affect the morphology of polyethylene wear debris?

Can centrifugation affect the morphology of polyethylene wear debris?

Metal-on-metal hip prostheses: Correlation between debris in the synovial fluid and levels of cobalt and chromium ions in the bloodstream

The evolution of polymer wear debris from total disc arthroplasty

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