A new method for the analysis of amikacin using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization and high-performance liquid chromatography with UV-detection

Ovalles, Fernando; Brunetto, Marı́a del Rosario; Gallignani, Máximo

doi:10.1016/j.jpba.2005.02.030

Cited by 25 publications

(19 citation statements)

References 20 publications

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“…When compared to other aminoglycosides amikacin has very narrow safety margin i.e., its therapeutic plasma concentration is 8-16 µg/ml. When it is given to renal impaired patients for over a period of time it shows ototoxicity and nephrotoxicity [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Teja

Gurupadayya

Sairam

2018

Int J Curr Pharm Sci

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Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based on simple charge transfer complexation of the drug with a p-chloranillic acid reagent to give a purple coloured product which was measured at 524nm against blank solution. Results:The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2. Conclusion:The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

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Section: Introductionmentioning

confidence: 99%

Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Teja

Gurupadayya

Sairam

2018

Int J Curr Pharm Sci

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“…Previously, amikacin has been detected using analytical methods such as capillary electrophoresis (Oguri & Miki, 1996;Yu et al, 2009) and liquid chromatography (Chen et al, 2012;Nicoli & Santi, 2006;Ovalles, Brunetto, & Gallignani, 2005;Zawilla et al, 2007). However, these methods are costly, time-consuming, require sophisticated laboratory equipment and technically trained personnel (Chen, Kong, et al, 2016;Li et al, 2016;Lourenco, Barbosa, & Pinto, 2011;Xu et al, 2016;Xu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the amikacin molecule has no chromophores that can give a reliable signal in the UV region, lacks volatility and is very hydrophilic (Farouk, Azzazy, & Niessen, 2015). As a result, direct HPLC analysis by UV detection is further complicated since it requires derivatization of some functional groups in amikacin to give efficient absorption in the UV-visible wavelength range or efficient fluorescent properties (Farouk et al, 2015;Ovalles et al, 2005;Zawilla et al, 2007). Therefore, other than the chromatographic methods, there is a need for alternative analytical techniques for amikacin determination.…”

Section: Introductionmentioning

confidence: 99%

Development of a monoclonal antibody assay and immunochromatographic test strip for the detection of amikacin residues in milk and eggs

Isanga

Mukunzi

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

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An amikacin-sensitive monoclonal antibody (MAb) assay and immunochromatographic test strip were developed and applied for the detection of amikacin residues in bovine milk and chicken eggs. The immunoassay was specific to amikacin and showed no cross-reactivity with other aminoglycosides. The half maximum inhibitory concentration (IC 50 ) of the assay was 0.65 ng/mL and the results were obtained within 90 min. Recoveries from spiked food matrices were within the range of 73.55-84.61% for bovine milk and 73.70-105.75% for whole egg. The strip test results were obtained within 10 min and showed a visual detection limit of 5.0 ng/mL for both food matrices. These results show that the MAb immunoassay and strip test developed in this study are very specific to amikacin and sufficiently sensitive for detection and routine monitoring of amikacin residues in food. ARTICLE HISTORY

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“…Amikacin sulfate is quantitatively determined through various analytical techniques such as spectrophotometry, 4-8 spectroflourimetry 9-13 and HPLC methods. [14][15][16][17][18][19][20][21][22][23][24][25] The aminoglycoside antibiotic activity can be increased by association with Herbs like activity by Pityrogramma calomelanos (L.). 26 The spectroscopic method for assay of amikacin sulfate is not official in any pharmacopoeia.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

Mughal¹,

Dayo²,

Ghoto³

et al. 2015

J YOUNG PHARM

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Objective: To determine the amikacin sulfate by developing a new spectrophotometric method by derivatization using double beam spectrophotometer from pure and commercial brands. Method: A quantitative analytical method was developed by amikacin sulfate using spectrophotometer after derivatization with vanillin under optimized parameters like pH, heating time and temperature, volume of reagent, Impact of mixing order, Effect of addition of solvents and Effect of excipients. The method was successfully applied on bulk and different brands containing amikacin sulfate. Results: The bulk and pharmaceutical analysis was carried out of different brands with formation of slight yellow colored imine base. The product showed absorbance at 400 nm w i t h molar absorptivity of 5.

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A new method for the analysis of amikacin using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization and high-performance liquid chromatography with UV-detection

Cited by 25 publications

References 20 publications

Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Spectrophotometric Method for the Determination Amikacin in Pure and Pharmaceutical Dosage Form

Development of a monoclonal antibody assay and immunochromatographic test strip for the detection of amikacin residues in milk and eggs

Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

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