A New Method for the Determination of γ-Glutamyltransferase in Serum

Persijn, J.-P.; Slik, W. van der

doi:10.1515/cclm.1976.14.1-12.421

Cited by 191 publications

(119 citation statements)

References 19 publications

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“…Gamma-glutamyltranferase (GMT, as an indicator of liver function) was biochemically determined in serum using Bio-La-test gamma-glutamyltransferase -GMT L250 kit (Erba Lachema, Brno, Czech Republic) on XL -200 analyser (Erba Mannheim, Mannheim, Czech Republic). Catalytic concentration of GMT was measured according to Persijn and Vanderslik (1976).…”

Section: Chemical Analysismentioning

confidence: 99%

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

Tůmová

Volek

Chodová

et al. 2016

Animal

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A 3 to 4 week feed restriction of about 20% to 25% of the free intake is widely applied in rabbit breeding systems to reduce postweaning digestive disorders. However, a short intensive feed restriction is described in few studies and can be beneficial for growing rabbits due to a longer re-alimentation period. The aim of this study was to evaluate the effect of ad libitum (AL) and two restriction levels of feeding (50 and 65 g/rabbit per day) applied for 1 week on performance, gastrointestinal morphology and physiological parameters during the restriction and during the re-alimentation period. Rabbits were divided into three experimental groups: AL rabbits were fed AL, R1 rabbits were restricted from 42 to 49 days of age and received 50 g daily (29% of AL) and R2 rabbits were restricted at the same age and were fed 65 g of feed daily (37% of AL). In the 1 st week after weaning and in the weeks after restriction, all the groups were fed AL. During the restriction period, daily weight gain (DWG) in R1 significantly dropped to 11% (experiment 1) and 5% (experiment 2) compared with rabbits in the AL group, although they were fed 29% of AL, whereas in the R2 group it decreased to 20% (experiment 1) and 10% (experiment 2). In the week following feed restriction, DWG in the restricted groups increased (P < 0.001) to 166% and 148% in R1 and to 128% and 145% in R2. Restricted rabbits in both the experiments reached up to 90% to 93% of the final live weight (70 days) of the AL group. Over the entire experimental period, feed restriction significantly decreased feed intake to 85% to 88% of the AL group; however, the feed conversion ratio was lower (P < 0.05) only in experiment 1 (−6% in R1 and −4% in R2). Digestibilities of CP and fat were not significantly higher during the restriction period and during the 1 st week of re-alimentation compared with the AL group. Significant interactions between feeding regime and age revealed the shortest large intestine in the AL group at 49 days of age and the longest at 70 days in the AL and R1 groups. Small intestinal villi were significantly higher and the crypts were significantly deeper in the restricted groups. It could be concluded that short intensive feed restriction increased digestible area in the small and large intestines, including the height of villi and depth of crypts, which might be involved in the compensatory growth and defence mechanism.

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Section: Chemical Analysismentioning

confidence: 99%

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

Tůmová

Volek

Chodová

et al. 2016

Animal

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“…8 The normal reference value of the GGT activity was 7-49 U/L. C-reactive protein (CRP) levels were measured on a Bio B (Roche Diagnostics, Mannheim, Germany) by means of turbidimetry.…”

Section: Biochemical Measurementsmentioning

confidence: 99%

The Importance of Gamma‐Glutamyltransferase Activity in Patients with Coronary Artery Disease

Demircan

Yazıcı

Durna

et al. 2009

Clinical Cardiology

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Background: In this study, we sought to investigate the relation of gamma-glutamyltransferase (GGT) levels with the significance of coronary artery disease (CAD), clinical presentation, left ventricular (LV) function, and inflammatory activity. Methods: A total of 235 patients (mean age: 60.1 ± 10.5 years, 166 [70%] males) who had coronary angiography were included in the study. Patients who had CAD constituted the study group (Group 1, n = 189) and patients who had insignificant coronary disease or normal coronary activity constituted the control group (Group 2, n = 46). Results: GGT levels were higher in Group 1 than Group 2 (38.7 ± 30.9 U/L versus 27.5 ± 17.5 U/L, p = 0.025). Left ventricular ejection fraction (LVEF) was lower in Group 1 than Group 2 (52.6%±11.7% versus 58.8%±11.3%, p<0.002). GGT activity (40.2 ± 32.5 U/L versus 29.1 ± 18.3 U/L, p<0.002) and c-reactive protein (CRP) levels (33.9 ± 43.6 mg/dl versus 17.8 ± 29.8 mg/dl, p<0.002) were higher, LVEF (52.6%±12.1% versus 56.5%±11.0%, p = 0.021) was lower in patients with acute coronary syndrome compared with stable CAD group. In regression analysis, CRP levels (p<0.0001, odds ratio [OR] = 3.77, 95% confidence interval [CI] 0.10

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“…Various synthetic substrates such as D, L-γ -glutamyl aminoproprionitril, N-(D, L-γ -glutamyl) aniline and L-γ -glutamyl-α-naphthylamide have been proposed [24].…”

Section: Introductionmentioning

confidence: 99%

“…Only L-γ -glutamyl-p-nitroanilide has been retained for routine use [20,24]. γ -GT activity is generally measured with L-γ -glutamylp-nitroanilide as the donor of the γ -glutamyl moiety and glycylglycine as the acceptor substrate.…”

Section: Introductionmentioning

confidence: 99%

Studies on the thermal sensitivity of ?-glutamyl transpeptidase measured with a modified test procedure and compared with that of alkaline phosphatase and lactoperoxidase in milk

Blel

Guingamp

Gaillard

et al. 2002

Lait

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-The milk enzyme γ -glutamyl transpeptidase (γ -GT) might be a useful indicator for monitoring the various conditions of milk pasteurisation. In the present work, γ -GT activity was measured using Clarifying Reagent ® to facilitate spectrophotometric measurement. The proposed method exhibited a good sensitivity (7.1 mU·mL -1 ) and a satisfying repeatability with a CV of 3% (n = 20). It was well correlated with the reference method for γ -GT activity measurement in 40 bovine milk samples (r = 0.98). The stability of γ -GT to milk heat treatments was compared with that of alkaline phosphatase (ALP) and lactoperoxidase. The thermal denaturation characteristics of γ -GT were very close to those of lactoperoxidase. γ -GT and ALP were of interest to control the label "made from raw milk" in Camembert cheese. This method could be suitable for routine quality control and assessment of mild heat treatment.Bovine milk / cheese / -glutamyl transpeptidase / alkaline phosphatase / lactoperoxidase / heat treatment Résumé -Étude de la thermosensibilité de la -glutamyl transpeptidase, mesurée à l'aide d'une méthode modifiée faisant appel à la transparisation, comparée à celles de la phosphatase alcaline et de la lactoperoxydase dans le lait. La γ -glutamyl transpeptidase (γ -GT) est une enzyme du lait qui pourrait être un indicateur utile pour la surveillance des conditions de pasteurisation du lait. Dans la méthode proposée, l'activité de la γ -GT a été mesurée en utilisant le Réactif de Transparisation ® pour faciliter la mesure spectrophotométrique. La méthode proposée présente une bonne sensibilité (7,1 mU·mL -1 ) et une répétabilité satisfaisante avec un CV de 3 % (n = 20). Les résultats de la mesure d'activité γ -GT de 40 échantillons de lait bovin obtenus par la méthode proposée sont en bonne corrélation avec ceux obtenus par la méthode de référence (r = 0,98). La stabilité de la γ -GT dans le lait soumis à différents traitements thermiques a été comparée à celles de la phosphatase alcaline (ALP) et de la lactoperoxydase. Les caractéristiques de la dénaturation thermique de la γ -GT étaient très proches de celles de la lactoperoxydase. Les activités γ -GT et ALP résiduelles pourraient être intéressantes pour le contrôle de l'appellation « au lait cru » du camembert. L'utilisation de cette méthode serait possible en analyse de routine pour le contrôle de la qualité ainsi que pour l'évaluation des traitements thermiques modérés.Lait / fromage / -glutamyl transpeptidase / phosphatase alcaline / lactoperoxydase / traitement thermique

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A New Method for the Determination of γ-Glutamyltransferase in Serum

Cited by 191 publications

References 19 publications

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

The Importance of Gamma‐Glutamyltransferase Activity in Patients with Coronary Artery Disease

Studies on the thermal sensitivity of ?-glutamyl transpeptidase measured with a modified test procedure and compared with that of alkaline phosphatase and lactoperoxidase in milk

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