A New Method to Determine the Level of Coenzyme Q10 in One Drop of Human Blood for Biomedical Research

Effects of simvastatin on blood lipids, vitamin E, coenzyme Q10 levels and left ventricular function in humans

“…The precipitates were reconstituted into 500 µ L of a 1 : 3 Hexane : Dichloromethane (H : D) solution and applied to Silica Sep-Pak Columns (690 mg) [17] which had been prewashed with 12·5 mL of the H : D solution. The columns were then washed with 3·5 mL of the H : D solution, with coenzyme Q 9 and coenzyme Q 10 being retained on the column.…”

Sensitive and selective analysis of coenzyme Q10in human serum by negative APCI LC-MS

“…Many procedures have been reported for the analysis of CoQ 10 in human plasma. [8][9][10][11][12][13][14][15][16][17][18][19][20][21] The total CoQ 10 concentration may be obtained by the simultaneous detection of both the reduced and oxidized CoQ 10 . [8][9][10][11][12][13][14][15][16] However, CoQ 10 H 2 is very unstable 10,17 and for routine clinical total CoQ 10 determinations of many samples a better strategy is to oxidise the reduced form to CoQ 10 before or during the extraction.…”

“…[8][9][10][11][12][13][14][15][16][17][18][19][20][21] The total CoQ 10 concentration may be obtained by the simultaneous detection of both the reduced and oxidized CoQ 10 . [8][9][10][11][12][13][14][15][16] However, CoQ 10 H 2 is very unstable 10,17 and for routine clinical total CoQ 10 determinations of many samples a better strategy is to oxidise the reduced form to CoQ 10 before or during the extraction. [15][16][17][18][19][20][21] The oxidation has been performed with O 2 , 14,[18][19][20] FeCl 3 , 11 CuCl 2 17 and 1,4-benzoquinone.…”

“…[8][9][10][11][12][13][14][15][16] However, CoQ 10 H 2 is very unstable 10,17 and for routine clinical total CoQ 10 determinations of many samples a better strategy is to oxidise the reduced form to CoQ 10 before or during the extraction. [15][16][17][18][19][20][21] The oxidation has been performed with O 2 , 14,[18][19][20] FeCl 3 , 11 CuCl 2 17 and 1,4-benzoquinone. 21 The total CoQ 10 concentrations are usually obtained by analysis of the extracts by reverse-phase, high-performance liquid chromatography (RP-HPLC) coupled either with a UV or electrochemical detector (ECD).…”

Биофармацевтический анализ коэнзима Q₁₀ (убидекаренона)

“…Äëÿ îñàaeäåíèÿ áåëêîâ èñïîëüçóþò ýòàíîë, ìåòàíîë, ïðîïàíîë-2 è èõ ñìåñè [11]. Ýòèëîâûé ñïèðò ÿâëÿåòñÿ íàèáîëåå ÷àñòî èñïîëüçóåìûì àãåíòîì äëÿ äåïðîòåíèíèçàöèè êðîâè [10,12,13,14,15], à òàêaeå ãîìîãåíàòîâ ðàçëè÷íûõ òêàíåé [5,6,7,9,16] âñëåäñòâèå äîñòóïíîñòè è íèçêîé òîêñè÷íîñòè.…”