A New Pyoverdin from Pseudomonas aeruginosa R'

Ruangviriyachai, Chalerm; Fernández, Diana Urı́a; Fuchs, Regine; Meyer, Jean‐Marie; Budzikiewicz, H.

doi:10.1515/znc-2001-11-1203

Cited by 19 publications

(14 citation statements)

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“…Previous work showed that RЈ synthesizes a rare fourth type of pyoverdine, a type III pyoverdine with a missing glutamine residue (61). Consistent with these findings, RЈ has a type III sequence type with a deletion of one nonribosomal peptide synthetase cassette, whose predicted specificity is glutamine.…”

supporting

confidence: 75%

Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa

et al. 2005

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Pyoverdine is the primary siderophore of the gram-negative bacterium Pseudomonas aeruginosa. The pyoverdine region was recently identified as the most divergent locus alignable between strains in the P. aeruginosa genome. Here we report the nucleotide sequence and analysis of more than 50 kb in the pyoverdine region from nine strains of P. aeruginosa. There are three divergent sequence types in the pyoverdine region, which correspond to the three structural types of pyoverdine. The pyoverdine outer membrane receptor fpvA may be driving diversity at the locus: it is the most divergent alignable gene in the region, is the only gene that showed substantial intratype variation that did not appear to be generated by recombination, and shows evidence of positive selection. The hypothetical membrane protein PA2403 also shows evidence of positive selection; residues on one side of the membrane after protein folding are under positive selection. R, previously identified as a type IV strain, is clearly derived from a type III strain via a 3.4-kb deletion which removes one amino acid from the pyoverdine side chain peptide. This deletion represents a natural modification of the product of a nonribosomal peptide synthetase enzyme, whose consequences are predictive from the DNA sequence. There is also linkage disequilibrium between the pyoverdine region and pvdY, a pyoverdine gene separated by 30 kb from the pyoverdine region. The pyoverdine region shows evidence of horizontal transfer; we propose that some alleles in the region were introduced from other soil bacteria and have been subsequently maintained by diversifying selection.

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supporting

confidence: 75%

Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa

et al. 2005

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“…Indeed, given the specificity for a membrane receptor that always has to recognize its pyoverdin, it seems probable that the pyoverdins evolve predominantly by replacement, loss, or acquisition of one amino acid. As shown with structurally related typical pyoverdins (2,31,33,39), the membrane receptor could probably still incorporate a slightly modified pyoverdin.…”

Section: Discussionmentioning

confidence: 88%

“…The results of the amino acid and MS analyses strongly suggest that such a modification probably occurred in the pyoverdins of P. syringae and P. cichorii, with replacement of a serine by a glycine or vice versa. Differences of one amino acid between typical pyoverdins were observed in P. aeruginosa and in P. fluorescens (31,39). In both cases, the pyoverdins differed essentially in terms of the presence of one additional amino acid in one of the molecules.…”

Section: Discussionmentioning

confidence: 99%

“…Fe(III) incorporation experiments are often carried out to confirm the results of IEF analyses (1,19,27,28). However, the same membrane receptor can incorporate different pyoverdins if they have some similarities (2,31,33,39), and bacteria can possess additional membrane receptors to incorporate heterogeneous pyoverdins (11,23). With P. syringae and P. cichorii, growth stimulation tests indicated that the two similar pyoverdins could be used by the two species (Bultreys and Gheysen, unpublished).…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, there are different siderovars in certain species, such as Pseudomonas fluorescens (about 19 siderovars) or Pseudomonas putida (about 13 siderovars) (14). The peptide chain of one of the four pyoverdins described for Pseudomonas aeruginosa (27,31) is synthesized by a nonribosomal pathway by means of the peptide synthetase PvdD (25). DNA hybridization studies have shown that genes comparable to pvdD are present in other fluorescent Pseudomonas species (25,30), but no clear evolutionary relationship has been found yet among the peptide chains of the pyoverdins described for different species (14).…”

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confidence: 99%

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High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species

Bultreys¹,

Gheysen²,

Wathelet

et al. 2003

Appl Environ Microbiol

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The relationship of pyoverdins produced by 41 pathovars of Pseudomonas syringae and by phytopathogenic Pseudomonas species was investigated. A high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical Fe(III)-chelated pyoverdins. Nonfluorescent strains were found in Pseudomonas amygdali, Pseudomonas meliae, Pseudomonas fuscovaginae, and P. syringae. Pseudomonas agarici and Pseudomonas marginalis produced typical pyoverdins. Among the arginine dihydrolase-negative fluorescent Pseudomonas species, spectral, amino acid, and mass spectrometry analyses underscored for the first time the clear similarities among the pyoverdins produced by related species. Within this group, the oxidase-negative species Pseudomonas viridiflava and Pseudomonas ficuserectae and the pathovars of P. syringae produced the same atypical pyoverdin, whereas the oxidase-positive species Pseudomonas cichorii produced a similar atypical pyoverdin that contained a glycine instead of a serine. The more distantly related species Pseudomonas asplenii and Pseudomonas fuscovaginae both produced a less similar atypical pyoverdin. The spectral characteristics of Fe(III)-chelated atypical pyoverdins at pH 7.0 were related to the presence of two ␤-hydroxyaspartic acids as iron ligands, whereas in typical pyoverdins one of the ligands is always ornithine based. The peptide chain influenced the chelation of iron more in atypical pyoverdins. Our results demonstrated that there is relative pyoverdin conservation in the amino acids involved in iron chelation and that there is faster evolution of the other amino acids, highlighting the usefulness of pyoverdins in systematics and in identification.

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Towards Raman‐based epidemiological typing of Pseudomonas aeruginosa

Willemse-Erix

Jachtenberg

Schut

et al. 2010

Journal of Biophotonics

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Raman spectra of bacteria can be used as highly specific fingerprints, enabling discrimination at strain level. Pseudomonas aeruginosa strains can be strongly pigmented, making it difficult to obtain high quality spectra of such isolates due to high fluorescent spectral backgrounds. Furthermore, the spectra that could be measured with acceptable quality often showed large spectral variations limiting the reproducibility required for strain level discrimination. P. aeruginosa produces a characteristic yellowish green fluorescent pigment, called pyoverdin. Applying a washing procedure to reduce the amount of fluorescent pigment, enabled the highly pigmented isolates to be measured with sufficient spectral quality. Isolation of the pigment/pyoverdin spectral features, together with spectral scaling methods improved reproducibility. It will be important to analyze the range of the spectral variations that can occur and ensure the correction of all of these factors to obtain the highest reproducibility required for strain level typing.

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A New Pyoverdin from Pseudomonas aeruginosa R'

Abstract: From a Pseudom onas aeruginosa hospital isolate a new pyoverdin was isolated. It is identical with that of Pseudomonas aeruginosa strain R except that in the peptide chain L-Gln is missing.

Cited by 19 publications

References 0 publications

Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa

Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa

High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species

Towards Raman‐based epidemiological typing of Pseudomonas aeruginosa

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A New Pyoverdin from Pseudomonas aeruginosa R'

Abstract: From a Pseudom onas aeruginosa hospital isolate a new pyoverdin was isolated. It is iden­tical with that of Pseudomonas aeruginosa strain R except that in the peptide chain L-Gln is missing.

Cited by 19 publications

References 0 publications

Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa

Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa

High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species

Towards Raman‐based epidemiological typing of Pseudomonas aeruginosa

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Product

Resources

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Abstract: From a Pseudom onas aeruginosa hospital isolate a new pyoverdin was isolated. It is identical with that of Pseudomonas aeruginosa strain R except that in the peptide chain L-Gln is missing.