A new species of Henneguya, a gill parasite of Astyanax altiparanae (Pisces: Characidae) from Brazil, with comments on histopathology and seasonality

Barassa, Bianca; Cordeiro, Nelson S.; Arana, Sarah

doi:10.1590/s0074-02762003000600009

Cited by 40 publications

(13 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding indicate that the life cycle of this parasite is not influenced by environmental factors or host development, as also reported by Barassa et al (2003) for H. chydadea Barassa, Arana et Cordeiro, 2003 infecting the gills of Astyanax altiparanae. In contrast, Molnár (1998) observed that the prevalence of H. creplini Gurley, 1894 varied with season and host size.…”

Section: Resultssupporting

confidence: 84%

Histophatology and ultrastructure of Henneguya caudalongula sp. n. infecting Prochilodus lineatus (Pisces: Prochilodontidae) cultivated in the state of São Paulo, Brazil

Adriano

Arana

Cordeiro

2005

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

Prochilodus lineatus (Valenciennes, 1836) is a detritivorous teleost popularly known as curimba or curimbatá, is widely distributed in South American rivers and attains a relatively large size, with some specimens measuring 75 cm in length and weighing 8.2 kg (Godoy 1975). It has a high reproductive capacity, rapid growth, good acceptance on the market and is one of the most consumed fish species in Brazil. Its consumption has led to increased interest in its cultivation in fish farms throughout the country.Until recently, Myxobolus porofilus Adriano, Aranas, Ceccarelli et Cordeiro, 2002 was the only myxosporean species reported to parasitize P. lineatus. However, during a survey of myxosporean parasites of fish species cultivated in Brazil, we found a new species of Henneguya parasitizing the gills of P. lineatus. This species is described based on light microscopy, scanning electron microscopy, transmission electron microscopy, and on the histopathological effects produced in the host. MATERIALS AND METHODSFour-month old specimens of P. lineatus (curimbatá), Piaractus mesopotamicus (Holmberg, 1887) (pacu), Brycon cephalus (Günther, 1869) (matrinxã) and Leporinus macrocephalus Garavello et Britski, 1988 (piauçu) obtained from artificial reproduction programs were released in a pond at the Center for the Research and Management of Continental Fishing Resources (Cepta/Ibama) located in the municipality of Pirassununga, state of São Paulo, Brazil. Each month, five specimens of each fish species were examined for the presence of myxozoan parasites from March 2000 to February 2002. Immediately after collection, the fish were transported alive to the laboratory where they were killed by transection of the spinal cord, before being measured and necropsied. Of 120 curimbatá examined, 20 were 7-15 cm long, 78 were 15.1-25 cm long and 22 were 25.1-30.5 cm long.The parasite was identified according to Lom and Arthur (1989). Measurements from 40 fresh mature spores were obtained using a ocular micrometer and are givem in micrometres (mm) as the mean ± standard deviation (SD). Smears containing free spores were stained with a Giemsa solution and mounted in low-viscosity mounting medium (Cytoseal) as permanent preparations. For histological analysis, the gills were fixed in 10% buffered formalin for 24 h, embedded in paraffin, cut into sections 4 µm thick and stained with sirius red (Adriano et al. 2002), haematoxylin and eosin, and PAS. For scanning electron microscopy, free spores were deposited on a coverslip coated with poly-L-lysine and fixed for 2 h at room temperature with glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2). After washing in the same buffer, the preparations were dehydrated in ethanol, critical point dried by CO 2 , coated with metallic gold and examined in JEOL JSM 35 microscope operated at 15 kV. For transmission electron microscopy, fragments of gills containing plasmodia were fixed in 2.5% glutaraldeyde in cacodylate buffer (2 h), dehydrated in increasing concentrations of acetone and embe...

show abstract

Section: Resultssupporting

confidence: 84%

Histophatology and ultrastructure of Henneguya caudalongula sp. n. infecting Prochilodus lineatus (Pisces: Prochilodontidae) cultivated in the state of São Paulo, Brazil

Adriano

Arana

Cordeiro

2005

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, poor water quality due to excesses of particulate material and/or pollutants also causes subepithelial edema, and this edema protects the integrity of the sinusoid capillaries in that it keeps them further away from the aggressive agent. The consequence of the edema is similar to that of hyperplasia, as previously observed in Piaractus mesopotamicus (Martins et al 1997(Martins et al , 1999a and in Astyanax altiparanae (Barassa et al 2003). …”

Section: Discussionsupporting

confidence: 84%

Myxosporidiosis in intensively-reared Piaractus mesopotamicus: Histopathological diagnosis by means of Ziehl-Neelsen staining

et al. 2012

View full text Add to dashboard Cite

Samples of different organs from intensively-reared Piaractus mesopotamicus were collected and processed using routine histological techniques in order to produce thin sections for staining with hematoxylin-eosin and with the Ziehl-Neelsen method. Through examination under an optical microscope, myxosporidians of the genera Henneguya sp. and Myxobolus sp. were identified, respectivelyin the gills and kidneys of P. mesopotamicus. Plasmodia with immature spores of Henneguya sp. were located along the secondary lamellae, with total length of 30.45±4.84µm and width of 3.52±0.33µm. Spores of Myxobolus sp. were located in the kidneys, with total length of 8.94±0.82µm and width of 5.59±0.39µm. Histopathological analysis of the gills showed plasmodia containing spores of Henneguya sp., at intralamellar and intravascular localities, at different stages of development. Spores of Myxobolus sp. were identified in the kidneys, in the peritubular region and in the interstices and glomerulus, surrounded by melanomacrophages. Focal hemorrhage was recorded in a few cases. Ziehl-Neelsen staining allowed to identify particular features of the spores and facilitated biometry and enabled classification in comparison with hematoxylin-eosin, thus demonstrating its usefulness for histopathological diagnosis of the parasitosis.

show abstract

“…In addition, a variable number of inflammatory cells occurred in the lamina propria-submucosa, as well as in the basal part of the lining epithelium. In several other myxosporean infections no inflammatory response was associated with the presence of the parasite (Moran et al 1999, Ali et al 2003, Barassa et al 2003, Adriano et al 2005. However, Enteromyxum scophthalmi can elicit a strong inflammatory host response in addition to the direct deleterious effects on the gastrointestinal mucosa.…”

Section: Discussionmentioning

confidence: 99%

“…These structures together with their junctions play roles in attachment and communication with host cells. Not only the acquisition of nutrients by the myxosporean through this linkage may contribute to the epithelial injury observed, but also the compression suffered by epithelial cells as the disease progresses and the number and size of parasitic forms increases (El-Mansy & Bashtar 2002, Barassa et al 2003.…”

Section: Discussionmentioning

confidence: 99%

Light and electron microscopic studies on turbot Psetta maxima infected with Enteromyxum scophthalmi: histopathology of turbot enteromyxosis

Bermúdez¹,

Losada²,

Vázquez³

et al. 2010

Dis. Aquat. Org.

View full text Add to dashboard Cite

In the last decade, a new parasite that causes severe losses has been detected in farmed turbot Psetta maxima (L.), in north-western Spain. The parasite was classified as a myxosporean and named Enteromyxum scophthalmi. The aim of this study was to characterize the main histological changes that occur in E. scophthalmi-infected turbot. The parasite provoked catarrhal enteritis, and the intensity of the lesions was correlated with the progression of the infection and with the development of the parasite. Infected fish were classified into 3 groups, according to the lesional degree they showed (slight, moderate and severe infections). In fish with slight infections, early parasitic stages were observed populating the epithelial lining of the digestive tract, without eliciting an evident host response. As the disease progressed, catarrhal enteritis was observed, the digestive epithelium showed a typical scalloped shape and the number of both goblet and rodlet cells was increased. Fish with severe infections suffered desquamation of the epithelium, with the subsequent release of parasitic forms to the lumen. The dislodged enterocytes underwent anoikis, a mode of apoptosis triggered by the loss of anchorage, which might facilitate spreading of the parasite. Lymphohaematopoietic depletion was also observed, mainly in head kidney and spleen, which could contribute to the high virulence of this parasite.

show abstract

A new species of Henneguya, a gill parasite of Astyanax altiparanae (Pisces: Characidae) from Brazil, with comments on histopathology and seasonality

Cited by 40 publications

References 10 publications

Histophatology and ultrastructure of Henneguya caudalongula sp. n. infecting Prochilodus lineatus (Pisces: Prochilodontidae) cultivated in the state of São Paulo, Brazil

Histophatology and ultrastructure of Henneguya caudalongula sp. n. infecting Prochilodus lineatus (Pisces: Prochilodontidae) cultivated in the state of São Paulo, Brazil

Myxosporidiosis in intensively-reared Piaractus mesopotamicus: Histopathological diagnosis by means of Ziehl-Neelsen staining

Light and electron microscopic studies on turbot Psetta maxima infected with Enteromyxum scophthalmi: histopathology of turbot enteromyxosis

Contact Info

Product

Resources

About