A new strain of docosahexaenoic acid producing microalga from Malaysian coastal waters

Manikan, Vidyah; Nazir, Mohamed Yusuf Mohamed; Kalil, Mohd Sahaid; Isa, Mohd Hafez Mohd; Kader, Abdul; Yusoff, Wan Mohtar Wan; Hamid, Aidil Abdul

doi:10.1016/j.algal.2015.02.023

Cited by 60 publications

(30 citation statements)

References 34 publications

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“…released zoospores from zoosporangium walls in two forms: some zoospores that split after being released, as previously reported[22]; other zoospores disintegrated after release, as reported by other authors[20]. The life cycle of A. limacinum BUCHAXM 122 was shown to be approximately 24 h. This study, therefore, suggests that cells www.scienceasia.org…”

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confidence: 85%

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Sirirak¹,

Suanjit²,

Powtongsook³

et al. 2020

ScienceAsia

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Aurantiochytrium limacinum BUCHAXM 122, a strain of thraustochytrid microorganism is potentially an alternative source of essential nutrients for commercial products. In this study, the strain was screened for its characterization and determination of polyunsaturated fatty acid (PUFA) production. Cells were isolated from fallen mangrove leaves, and grown in a glucose-yeast extract-peptone (GYP) medium. Their morphology, life cycle, biomass, fatty acid, extracellular enzymes and major elements were also investigated. Colonies occurred prominently on the fourth day of cultivation, appearing opaque white in color and containing large numbers of amoeboid cells. These thraustochytrids were observed to have a multi-stage life cycle, developing through zoospore, vegetative and zoosporangium stages with a life span of approximately 24 h. Zoospores developed into vegetative cells within 10-12 h and vegetative cells developed into zoosporangia, releasing zoospores, within approximately 10-15 h. Results showed that A. limacinum BUCHAXM 122 produced highest biomass at 108 h, with 23.85 ± 1.02 g/l dry weight. The isolates had high content of docosahexaenoic acid (DHA; 30.31 ± 5.88% of total fatty acids), indicating that they could serve as a fatty acid source for human consumption and aquaculture feeds. Major elemental analysis showed that cells contained 57% carbon, 29% oxygen, 9% hydrogen, 4% nitrogen and 1% sulfur. Extracellular degradative enzymes including protease, lipase, urease, α-glucosidase and phosphatase activities were also detected.

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supporting

confidence: 85%

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Sirirak¹,

Suanjit²,

Powtongsook³

et al. 2020

ScienceAsia

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“…When cultivated in artificial media containing yeast extract or peptone (for a review see [52]), thraustochytrids can accumulate large amounts of lipids as well as produce high biomass [53], which makes them very attractive for biotechnological applications. Indeed, when grown in the appropriate conditions, thraustochytrids from the genera Aurantiochytrium and Schizochytrium may accumulate lipids up to 30-50% of their dry weight, with DHA representing about half of the total FAs [6,19,35,62,63]. Lipid production depends on the composition of the medium [31,64], and thus varies along the life cycle [53].…”

Section: Lipids and The Life Cyclementioning

confidence: 99%

“…Besides their ecological relevance, thraustochytrids have attracted biotechnological interest because they naturally accumulate high levels of triacylglycerols (TAGs), like many other microalgae [30]. The peculiarity of thraustochytrids is their very high content in very long chains of polyunsaturated fatty acids (VLCPUFA), mainly ω3-docosahexaenoic acid (DHA, 22:6) [6,[31][32][33][34][35]. DHA and other ω3-PUFAs such as eicosapentaenoic acid (EPA, 20:5) are synthesized at extremely low levels in animals and are therefore considered 'conditionally essential' fatty acids (FAs) [36], which means they must be obtained from the diet.…”

Section: Introductionmentioning

confidence: 99%

The lipid metabolism in thraustochytrids

Morabito

Bournaud

Maës

et al. 2019

Progress in Lipid Research

139

100

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Thraustochytrids are unicellular heterotrophic marine protists of the Stramenopile group, often considered as non-photosynthetic microalgae. They have been isolated from a wide range of habitats including deep sea, but are mostly present in waters rich in sediments and organic materials. They are abundant in mangrove forests where they are major colonizers, feeding on decaying leaves and initiating the mangrove food web. Discovered 80 years ago, they have recently attracted considerable attention due to their biotechnological potential. This interest arises from their fast growth, their specific lipid metabolism and the improvement of the genetic tools and transformation techniques. These organisms are particularly rich in ω3-docosahexaenoic acid (DHA), an 'essential' fatty acid poorly encountered in land plants and animals but required for human health. To produce their DHA they use a complex system different from the classical fatty acid synthase system. They are also a potential source of squalene and carotenoids. Here we review our current knowledge about the life cycle, ecophysiology, and metabolism of these organisms, with a particular focus on lipid dynamics. We describe the different pathways involved in lipid and fatty acid syntheses, emphasizing their specificity, and we report on the recent efforts aimed to engineer their lipid metabolism.

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“…Therefore, the aim of this study is to optimize fructose and MSG which are the major carbon and nitrogen source in the cultivation media as well as agitation speed to enhance growth, lipid and DHA production of locally isolated Auranthiochytrium SW1 by using RSM. Fructose was used instead of glucose and other carbon sources is in conjunction from our previous experiment which concluded that fructose is the best carbon sources for growth, lipid and DHA production by this Thraustochytrids strain 17 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of Culture Conditions for Enhanced Growth, Lipid and Docosahexaenoic Acid (DHA) Production of Aurantiochytrium SW1 by Response Surface Methodology

Nazir

Shuib

Kalil

et al. 2018

Sci Rep

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In this study, optimization of growth, lipid and DHA production of Aurantiochytrium SW1 was carried out using response surface methodology (RSM) in optimizing initial fructose concentration, agitation speed and monosodium glutamate (MSG) concentration. Central composite design was applied as the experimental design and analysis of variance (ANOVA) was used to analyze the data. ANOVA analysis revealed that the process which adequately represented by quadratic model was significant (p < 0.0001) for all the response. All the three factors were significant (p < 0.005) in influencing the biomass and lipid data while only two factors (agitation speed and MSG) gave significant effect on DHA production (p < 0.005). The estimated optimal conditions for enhanced growth, lipid and DHA production were 70 g/L fructose, 250 rpm agitation speed and 10 g/L MSG. Consequently, the quadratic model was validated by applying the estimated optimum conditions, which confirmed the model validity where 19.0 g/L biomass, 9.13 g/L lipid and 4.75 g/L of DHA were produced. The growth, lipid and DHA were 28, 36 and 35% respectively higher than that produced in the original medium prior to optimization.

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A new strain of docosahexaenoic acid producing microalga from Malaysian coastal waters

Cited by 60 publications

References 34 publications

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

The lipid metabolism in thraustochytrids

Optimization of Culture Conditions for Enhanced Growth, Lipid and Docosahexaenoic Acid (DHA) Production of Aurantiochytrium SW1 by Response Surface Methodology

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