A new sulfated β-galactan from clams with anti-HIV activity

Chaidedgumjorn, Amornrut; Toida, Toshihiko; Imanari, Toshio; Woo, Eun‐Rhan; Park, Hokoon; Linhardt, Robert J.; Wu, Song; Kim, Yeong Shik

doi:10.1016/s0008-6215(99)00188-3

Cited by 65 publications

(20 citation statements)

References 19 publications

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“…Uronic acid content was measured as described by Bitter and Muir (1962). The neutral sugars were determined by converting them into acetylated aldononitrile derivatives and detected by GC (Amornrut et al, 1999). GC was performed with flame ionization detection on a Hitachi G-3000 gas chromatography equipped with DB-1 fused silica capillary column (375 µm i.d.…”

Section: Methodsmentioning

confidence: 99%

Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta)

Mao

Li-gen

et al. 2005

J Appl Phycol

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Polysaccharide from the green alga Monostroma angicava was extracted with boiling water and was purified by ion-exchange and size-exclusion column chromatography. The radioprotective effect of the polysaccharide was investigated in mice. The results show that polysaccharide from M. angicava has a different chemical composition to other Chlorophyta having a high rhamnose -containing sulfated polysaccharide. The sulfate ester content was estimated to be 21.8%. When the polysaccharide was applied to BALB/c mice following whole-body X-ray irradiation the counts of leukocytes, thrombocytes and erythrocytes recovered more rapidly in the polysaccharide treated mice after irradiation. In the irradiated mice, the polysaccharide significantly increased the spleen index, natural killer cytostatic activity and the transformation response of splenic lymphocytes. The present observations suggest that polysaccharide from M. angicava led to leukocytogensis and hematopoetic activation in mice after irradiation and that the biological response might be caused by immune activation.

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Section: Methodsmentioning

confidence: 99%

Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta)

Mao

Li-gen

et al. 2005

J Appl Phycol

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“…Ash was quantified gravimetrically after 12 h at 550 • C and further 4 h at 900 • C. Protein content was determined as described by Bradford (1976). The neutral sugars were determined by converting them into acetylated aldononitrile derivatives and detected by gas chromatography (Amornrut et al, 1999). Briefly, 10 mg polysaccharides was hydrolyzed in 2 mol L −1 sulfuric acid at 105 • C for 10 h. The hydrolysate was neutralized to pH 7.0 with barium carbonate and the precipitate was removed by centrifugation.…”

Section: Composition Analysismentioning

confidence: 99%

Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity

et al. 2006

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Sulfated polysaccharides from the green alga Ulva conglobata were isolated and prepared by extraction in hot water, precipitation with ethanol and purification by ion-exchange and size-exclusion column chromatography. The characterizations of the sulfated polysaccharides were defined, and containing 23.04-35.20% sulfate ester groups, 10.82-14.91% uronic acid and 3.82-4.51% protein. Gas chromatography analysis shows that the sulfated polysaccharides from Ulva conglobata are mainly consisted of rhamnose with variable contents of glucose and fucose, trace amounts of xylose, glactose and mannose. The anticoagulant properties of the sulfated polysaccharides were compared with those of heparin by studying the activated partial thromboplastin time using normal human plasma. The sulfated polysaccharide from Ulva conglobata collected in Qingdao, China is the most potent among the sulfated polysaccharides tested. The mechanism of anticoagulant activity mediated by the sulfated polysaccharides is due to the direct inhibition of thrombin and the potentiation of heparin cofactor II.

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“…Many studies focus on the potential use of polysaccharides as the carrier to target drugs to the colon [2]. Recently, the most interesting and intensive researches mainly focus on the bioactivities of polysaccharides and their chemical derivatives, especially sulfated derivatives [3][4][5][6][7]. It is also known that the structure and physico-chemical properties are the major factors affecting the biological activity of polysaccharides [8].…”

Section: Introductionmentioning

confidence: 99%

Molecular mass and antitumor activities of sulfated derivatives of α-glucan from Poria cocos mycelia

Lin

Zhang

Chen

et al. 2004

International Journal of Biological Macromolecules

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A new sulfated β-galactan from clams with anti-HIV activity

Cited by 65 publications

References 19 publications

Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta)

Chemical characterization and radioprotective effect of polysaccharide from Monostroma angicava (Chlorophyta)

Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity

Molecular mass and antitumor activities of sulfated derivatives of α-glucan from Poria cocos mycelia

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