A new tropical cyanobacterium Pinocchia polymorpha gen. et sp. nov. derived from the genus Pseudanabaena

Dvořák, Petr; Jahodářová, Eva; Hašler, Petr; Gusev, Evgeniy; Poulíčková, Aloisie

doi:10.5507/fot.2015.010

Cited by 37 publications

(15 citation statements)

References 28 publications

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“…The phylogram generated in this study suggests the existence of two more genera in this family. The genus Pinocchia ( Dvorak et al, 2015 ) is still classified within the family Leptolyngbyaceae , which contradicts the 16S-based phylogeny obtained in this study. Therefore, we propose that the genus Pinocchia should be reclassified into the family Trichocoleusaceae .…”

Section: Resultscontrasting

confidence: 99%

“…The relevant biosynthetic genes encoding cyanophycin synthase ( cphA ) and cyanophycinase ( cphB ) were identified in the genome. The carboxysomes were observed in the cytoplasm in small numbers, similar to related Pinocchia strains ( Dvorak et al, 2015 ; Kim et al, 2021 ). Gas vesicles and polyphosphate granules were also observed in the cytoplasm ( Figure 7D ).…”

Section: Resultssupporting

confidence: 64%

“…In the present study, the phylogeny of 16S-23S ITS has not been reconstructed mainly for two reasons. First, the 16S-23S ITS of important reference cyanobacteria, Pinocchia strains, only contained conserved tRNA lle , while another conserved tRNA Ala was missing ( Dvorak et al, 2015 ). Second, the 16S-23S ITS of strain B231 lacks the V2 region ( Table 3 ); indicating significant operon heterogeneity in these strains.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of a novel thermophilic cyanobacterium within Trichocoleusaceae, Trichothermofontia sichuanensis gen. et sp. nov., and its CO2-concentrating mechanism

et al. 2023

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Thermophiles from extreme thermal environments have shown tremendous potential regarding ecological and biotechnological applications. Nevertheless, thermophilic cyanobacteria remain largely untapped and are rarely characterized. Herein, a polyphasic approach was used to characterize a thermophilic strain, PKUAC-SCTB231 (hereafter B231), isolated from a hot spring (pH 6.62, 55.5°C) in Zhonggu village, China. The analyses of 16S rRNA phylogeny, secondary structures of 16S-23S ITS and morphology strongly supported strain B231 as a novel genus within Trichocoleusaceae. Phylogenomic inference and three genome-based indices further verified the genus delineation. Based on the botanical code, the isolate is herein delineated as Trichothermofontia sichuanensis gen. et sp. nov., a genus closely related to a validly described genus Trichocoleus. In addition, our results suggest that Pinocchia currently classified to belong to the family Leptolyngbyaceae may require revision and assignment to the family Trichocoleusaceae. Furthermore, the complete genome of Trichothermofontia B231 facilitated the elucidation of the genetic basis regarding genes related to its carbon-concentrating mechanism (CCM). The strain belongs to β-cyanobacteria according to its β-carboxysome shell protein and 1B form of Ribulose bisphosphate Carboxylase-Oxygenase (RubisCO). Compared to other thermophilic strains, strain B231contains a relatively low diversity of bicarbonate transporters (only BicA for HCO3− transport) but a higher abundance of different types of carbonic anhydrase (CA), β-CA (ccaA) and γ-CA (ccmM). The BCT1 transporter consistently possessed by freshwater cyanobacteria was absent in strain B231. Similar situation was occasionally observed in freshwater thermal Thermoleptolyngbya and Thermosynechococcus strains. Moreover, strain B231 shows a similar composition of carboxysome shell proteins (ccmK1-4, ccmL, -M, -N, -O, and -P) to mesophilic cyanobacteria, the diversity of which was higher than many thermophilic strains lacking at least one of the four ccmK genes. The genomic distribution of CCM-related genes suggests that the expression of some components is regulated as an operon and others in an independently controlled satellite locus. The current study also offers fundamental information for future taxogenomics, ecogenomics and geogenomic studies on distribution and significance of thermophilic cyanobacteria in the global ecosystem.

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Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 64%

Section: Resultsmentioning

confidence: 99%

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Characterization of a novel thermophilic cyanobacterium within Trichocoleusaceae, Trichothermofontia sichuanensis gen. et sp. nov., and its CO2-concentrating mechanism

et al. 2023

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“…Species concepts within the cyanobacteria are subject to much debate (Dvo r ak et al 2015b). Given the dearth of morphological features from which to choose, coupled with issues related to both phenotypic plasticity and cryptic diversity, describing and elucidating cyanobacterial species may be problematic (Dvo r ak et al 2015a,b, Dvo r ak et al 2017, Struneck y et al 2017. The monophyletic species concept sensu Johansen and has been proposed as the standard for cyanobacterial systematics (e.g., Kom arek 2013), which requires the description of an autapomorphy to test phylogenetic hypotheses.…”

Section: Discussionmentioning

confidence: 99%

Brasilonema lichenoides sp. nov. and Chroococcidiopsis lichenoides sp. nov. (Cyanobacteria): two novel cyanobacterial constituents isolated from a tripartite lichen of headstones

Villanueva

Hašler

Dvořák

et al. 2018

Journal of Phycology

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Cyanolichens are an assemblage of fungi and cyanobacteria from diverse, cosmopolitan habitats. Typically composed of a single species of cyanobacterium, with or without another eukaryotic alga, here we present two novel cyanobionts isolated from an undescribed tripartite lichen. This endolithic lichen was isolated from a granite cemetery tombstone from Jacksonville, FL, and contains two potentially nitrogen-fixing cyanobionts. Employing a total evidence approach, we characterized the cyanobionts using molecular (the 16S rDNA and ITS gene region), morphological, and ecological data. Phylogenetic analyses revealed two novel taxa: Brasilonema lichenoides and Chroococcidiopsis lichenoides, both of which fell within well-supported clades. To our knowledge, this represents the first instance of a tripartite lichen with two cyanobacterial and no eukaryotic members. These types of lichens may well represent an unexplored reservoir of cyanobacterial diversity. The specific epithets are proposed under the provisions of the International Code of Nomenclature for algae, fungi, and plants.

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“…In most instances, when new genera have been described, they contain only a single new species (Casamatta et al 2006, Joosten 2006, Fiore et al 2007, Reh akov a et al 2007, Lamprinou et al 2011, 2012, Novis and Visnovsky 2011, Dadheech et al 2012, Thu et al 2012, Zammit et al 2012, Nguyen et al 2013, Ha sler et al 2014, Wilde et al 2014, Dvo rak et al 2015, Song et al 2015, Vaz et al 2015, Alvarenga et al 2016, Jahod arov a et al 2017, Sciuto et al 2017, Cai et al 2018a,b, 2019, 2020a,b,c, Zhou et al 2018, and many more). Multiple species have sometimes been described with the description of a new genus (Engene et al 2012, Gonz alez-Resendiz et al 2018a, Kabirnataj et al 2018, Mai et al 2018, Saraf et al 2018a, 2019a, Pietrasiak et al 2019, 2021, but it has been more common to have multiple species in a new genus consisting of multiple new combinations as a result of revision of polyphyletic genera (e.g., Wacklin et al 2009, Zapom elov a et al 2010, Zapom elov a et al 2012, Struneck y et al 2017.…”

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confidence: 99%

When will taxonomic saturation be achieved? A case study in Nunduva and Kyrtuthrix (Rivulariaceae, Cyanobacteria)

Johansen

González-Resendiz

Escobar‐Sánchez

et al. 2021

Journal of Phycology

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A number of heterocytous, mat-forming, tapering cyanobacteria in Rivulariaceae have recently been observed in both the Atlantic and Pacific coasts in the rocky intertidal and supratidal zones. These belong to the genera Nunduva, Kyrtuthrix, and Phyllonema and have been the subject of several recent studies. Herein, two new species of Nunduva (N. komarkovae and N. sanagustinensis) and two new species of Kyrtuthrix (K. munecosensis and K. totonaca) are characterized and described from the coasts of Mexico. Genetic separation based on the 16S-23S ITS region was pronounced (>10% in all comparisons). Morphological differences between all existing species in these two genera were also observed, but the group is morphologically complex, and these taxa are considered pseudocryptic. Nunduva and Kyrtuthrix remain morphologically and phylogenetically separate even with the addition of new species. However, how long will this remain the case? Many new genera and species of cyanobacteria have recently been described. Will the taxonomy of cyanobacteria eventually become saturated? Will we start to see multiple populations for the same cryptic species, or will future taxonomists collapse multiple species into fewer species, or multiple genera into single genera. The description of even more Nunduva and Kyrtuthrix species causes us to pause and evaluate the future of cyanobacterial taxonomy. These same questions are faced by algal taxonomists studying other phyla, and the resolution may ultimately be similar.

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A new tropical cyanobacterium Pinocchia polymorpha gen. et sp. nov. derived from the genus Pseudanabaena

Cited by 37 publications

References 28 publications

Characterization of a novel thermophilic cyanobacterium within Trichocoleusaceae, Trichothermofontia sichuanensis gen. et sp. nov., and its CO2-concentrating mechanism

Characterization of a novel thermophilic cyanobacterium within Trichocoleusaceae, Trichothermofontia sichuanensis gen. et sp. nov., and its CO2-concentrating mechanism

Brasilonema lichenoides sp. nov. and Chroococcidiopsis lichenoides sp. nov. (Cyanobacteria): two novel cyanobacterial constituents isolated from a tripartite lichen of headstones

When will taxonomic saturation be achieved? A case study in Nunduva and Kyrtuthrix (Rivulariaceae, Cyanobacteria)

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