A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide

Zhao, Xihua; Wang, Wei; Tong, Bin; Zhang, Suping; Wei, Dongzhi

doi:10.1007/s12010-015-1866-x

Cited by 24 publications

(20 citation statements)

References 35 publications

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“…and Penicillium sp. can produce proteases that degrade cellulose (Zhao et al 2016; Thongekkaew et al 2013), dairy products, and polysaccharides (Inoue et al 2015). Fusarium sp.…”

Section: Discussionmentioning

confidence: 99%

“…Endophytic fungi can combine with other endogenous microorganisms and antibacterial compounds to form a defense system that produces alkaloids to strengthen immunity and maintain growth under stress (Qin et al 2011; Clay and Holah 1999). Some endophytic fungi produce active proteases helped maintain plant activities, such as pectinase and esterase, which degrade cell walls (Zhao et al 2016). Pathology can result when programmed senescence in the plant or environmental change perturb the fungal population in the plant (Stamford et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Gong

Zhou

Xiao

et al. 2019

World J Microbiol Biotechnol

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Dipsacus asperoides contains multiple pharmacologically active compounds. The principal are saponins. The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. However, the endophytic fungi of D. asperoides have not been analyzed in detail. In this study, we isolated and identified 46 endophytic fungal strains from the taproots, lateral roots and leaves, and we used morphological and molecular biological methods to assign them into 15 genera: Fusarium sp., Ceratobasidium sp., Chaetomium sp., Penicillium sp., Aspergillus sp., Talaromyces sp., Cladosporium sp., Bionectria sp., Mucor sp., Trichoderma sp., Myrothecium sp., Clonostachys sp., Ijuhya sp., Leptosphaeria sp. and Phoma sp. Taproots contained abundant endophytic fungi, the numbers of which correlated positively with level of dipsacus saponin VI. Primary fermentation of several endophytic fungal strains from taproots showed that Fusarium, Leptosphaeria, Ceratobasidium sp. and Phoma sp. can produce the triterpenoid saponin. These results may guide efforts to sustainably produce bioactive compounds from D. asperoides . Graphical abstract Electronic supplementary material The online version of this article (10.1007/s11274-019-2616-y) contains supplementary material, which is available to authorized users.

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“…and Penicillium sp. can produce proteases that degrade cellulose (Zhao et al 2016; Thongekkaew et al 2013), dairy products, and polysaccharides (Inoue et al 2015). Fusarium sp.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Gong

Zhou

Xiao

et al. 2019

World J Microbiol Biotechnol

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show abstract

“…DNS spectrophotometry is a widely accepted and applied method in research (Aoyama et al, 2015;Miller, 1959;Wood and Bhat, 1988;Xiao et al, 2004;Zhao et al, 2016), which determined the ability of DNS to saccharify CMC via the analysis of its hydrolysis products, such as glucose and cellobiose (reducing sugars).…”

Section: Optimization Of Measuring the Wavelength For Cellulase Activitymentioning

confidence: 99%

Characteristics of cellulase in cellulose-degrading bacterium strain Clostridium straminisolvens (CSK1)

Wang¹,

Hua²,

Wang³

et al. 2017

Afr. J. Microbiol. Res.

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Microbial degradation of biomass was considered as a clean and highly efficient approach to produce bioenergy, as it could mitigate the urgent demand for limited petroleum and natural gas. A thermophilic microbial consortium (MC1) was proved a high efficiency cellulose-degrading bacterial community in previous studies. A novel anaerobic, thermophilic, and cellulolytic bacterium (strain CSK1) was isolated from MC1. The cellulase activity characteristics of CSK1 were analyzed and evaluated by exploring new measuring conditions via the 3,5-dinitrosalicylic acid (DNS) spectrophotometry and the carboxymethyl cellulose (CMC) saccharogenic power method. The results indicated that the optimal measuring wavelength, reaction temperature, and pH value, were 530 nm, 60°C and 6.0, respectively. The ideal reaction time to achieve stable and significant measuring cellulase activity was about 10 min. Cellulase of CSK1 remained stable when the temperature was below 70°C, and the pH between 5.0 and 10.0, and its activity was quickly reduced when the temperature and pH exceeded such ranges. The cellulase activity of CSK1 reached the highest level on culturing day 8, and high correlations were found among cellulase activity variation, pH and CSK1 biomass change.

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“…Cell-free medium was collected after centrifugation at 6000 g per 20 min. Enzyme activity was measured according to Zhao et al (2016) with some modifications. Endoglucanase activity was determined by incubating 900 μL of CMC (1%) with enzymatic extract to a final volume of 1.1 mL containing 0.1 M acetate buffer, pH 6.0, at 50 °C for 50 min.…”

Section: Enzymatic Activitymentioning

confidence: 99%

Effect of CMC and MCC as Sole Carbon Sources on Cellulase Activity and eglS Gene Expression in Three Bacillus subtilis Strains Isolated from Corn Stover

et al. 2016

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a Cellulolytic activities in Bacillus subtilis have been demonstrated and it is known that the eglS gene encodes an endoglucanase that could play a key role. Three Bacillus subtilis strains (RZ164, RS351, and RS273) isolated from corn stover with contrasting cellulase activity were examined in this work. The aim was to analyze the influence of eglS gene on the ability of bacteria to grow on a liquid medium supplied with carboxymethyl cellulose (CMC) or microcrystalline cellulose (MCC) as sole carbon sources. All strains displayed similar growth in CMC medium and comparable exoglucanase and endoglucanase activity. However, the expression of eglS did not correlate among strains. On the other hand, when MCC was the carbon source tested, the growth of RS351 was higher than that obtained by RZ164 and RS273 strains. This behavior could be related to the level of cellulase activities displayed by this strain. Besides, eglS expression was higher in RS351 strain, suggesting a direct participation of this enzyme when the carbon source is MCC. Taken together, eglS could be involved in different roles exerted by these strains on either exo-or endoglucanase activity and under either substrate. The enzymes described here could be considered good alternatives for biomass conversion.

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A Newly Isolated Penicillium oxalicum 16 Cellulase with High Efficient Synergism and High Tolerance of Monosaccharide

Cited by 24 publications

References 35 publications

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Characteristics of cellulase in cellulose-degrading bacterium strain Clostridium straminisolvens (CSK1)

Effect of CMC and MCC as Sole Carbon Sources on Cellulase Activity and eglS Gene Expression in Three Bacillus subtilis Strains Isolated from Corn Stover

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