A Non-Invasive Hair Test to Determine Vitamin D3 Levels

Shah, Iltaf; Mansour, Mohamed H.; Jobe, Sheikh O.; Salih, E. T. Y.; Naughton, Declan P.; Ashraf, S. Salman

doi:10.3390/molecules26113269

Cited by 5 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various methods have been proposed for the non-invasive measurement of vitamin D. Methods for measuring vitamin D that do not use blood include skin color [31], body mass index [32], and hair [9]. The correlation with the amount of vitamin D in the blood was confirmed using saliva [33], and skin autofluorescence was confirmed to be related to vitamin D through an AEG reader in patients with type 2 diabetes [34].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, most of these methods use blood samples and put considerable Sensors 2021, 21, 8118 2 of 12 pressure on users. A non-invasive method of measuring vitamin D using hair sample exists, but the analysis uses classical methods, such as HPLC [9]. Other conditions that predict vitamin D levels include normal blood levels of calcium and phosphorus, high or high levels of parathyroid hormone, normal to high levels of total alkaline phosphatase, urinary calcium excretion rates, and vitamin D binding protein analysis [6,10,11].…”

Section: Introductionmentioning

confidence: 99%

“…A blood test is an accurate way to measure the amount of vitamin D in the body. Methods for indirectly measuring vitamin D include hair analysis [9] and Insufficiency Prediction score reflects individual characteristics using a multivariate logistic regression model for overweight, physical activity, winter season, sun exposure, etc. [12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Biosensor and Algorithm to Predict Vitamin D Status by Measuring Skin Impedance

Heo

Kim

et al. 2021

Sensors

View full text Add to dashboard Cite

The deficiency and excess of vitamin D cause various diseases, necessitating continuous management; but it is not easy to accurately measure the serum vitamin D level in the body using a non-invasive method. The aim of this study is to investigate the correlation between vitamin D levels, body information obtained by an InBody scan, and blood parameters obtained during health checkups, to determine the optimum frequency of vitamin D quantification in the skin and to propose a vitamin D measurement method based on impedance. We assessed body composition, arm impedance, and blood vitamin D concentrations to determine the correlation between each element using multiple machine learning analyses and an algorithm which predicted the concentration of vitamin D in the body using the impedance value developed. Body fat percentage obtained from the InBody device and blood parameters albumin and lactate dehydrogenase correlated with vitamin D level. An impedance measurement frequency of 21.1 Hz was reflected in the blood vitamin D concentration at optimum levels, and a confidence level of about 75% for vitamin D in the body was confirmed. These data demonstrate that the concentration of vitamin D in the body can be predicted using impedance measurement values. This method can be used for predicting and monitoring vitamin D-related diseases and may be incorporated in wearable health measurement devices.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Biosensor and Algorithm to Predict Vitamin D Status by Measuring Skin Impedance

Heo

Kim

et al. 2021

Sensors

View full text Add to dashboard Cite

show abstract

“…The second method in the literature was presented by Shah et al, who washed 500 mg of hair using a mixture of methanol:water and then analysed 200 mg of the cleaned hair, which was previously pulverized [ 89 ]. As the more polar nature of the washing solvent could potentially result in extraction of the analytes of interest, the washing solutions were analysed after the washing step.…”

Section: Sample Preparation Of Alternative/non-conventional Biologica...mentioning

confidence: 99%

Sample preparation techniques for extraction of vitamin D metabolites from non-conventional biological sample matrices prior to LC–MS/MS analysis

Αλεξανδρίδου

Volmer

2022

Anal Bioanal Chem

View full text Add to dashboard Cite

The determination of vitamin D metabolites as status marker or for diagnostic purposes is almost entirely conducted from blood serum or plasma. Other biological matrices, however, have also interested researchers, for two main reasons: (1) alternative matrices may allow non-invasive sampling, permit easier sample transfer and require less demanding storage conditions; and (2) the levels of vitamin D metabolites in other body compartments may further aid the understanding of vitamin D metabolism and function. Thus, the development of reliable and efficient sample preparation protocols for sample matrices other than serum/plasma, which will remove potential interferences and selectively extract the targeted metabolites, is of great importance. This review summarizes sample preparation methods for measurement of vitamin D metabolites using liquid chromatography-(tandem)mass spectrometry in more than ten different human tissues, including hair, saliva, adipose tissue, brain and others. Graphical abstract

show abstract

“…Zgaga et al (2019) [ 31 ] and another study published by our lab recently [ 32 ] analysed and quantified 25-hydroxyvitamin D concentrations in human hair using LC/MS-MS. These are the first two studies to use human hair and beard samples for LC/MS-MS to quantify 25(OH)D3 and to show the existence of 25(OH)D3 in human hair samples.…”

Section: Introductionmentioning

confidence: 99%

Determination of Vitamin B3 Vitamer (Nicotinamide) and Vitamin B6 Vitamers in Human Hair Using LC-MS/MS

et al. 2021

Self Cite

View full text Add to dashboard Cite

Water-soluble B vitamins participate in numerous crucial metabolic reactions and are critical for maintaining our health. Vitamin B deficiencies cause many different types of diseases, such as dementia, anaemia, cardiovascular disease, neural tube defects, Crohn’s disease, celiac disease, and HIV. Vitamin B3 deficiency is linked to pellagra and cancer, while niacin (or nicotinic acid) lowers low-density lipoprotein (LDL) and triglycerides in the blood and increases high-density lipoprotein (HDL). A highly sensitive and robust liquid chromatography–tandem mass spectroscopy (LC/MS-MS) method was developed to detect and quantify a vitamin B3 vitamer (nicotinamide) and vitamin B6 vitamers (pyridoxial 5′-phosphate (PLP), pyridoxal hydrochloride (PL), pyridoxamine dihydrochloride (PM), pridoxamine-5′-phosphate (PMP), and pyridoxine hydrochloride (PN)) in human hair samples of the UAE population. Forty students’ volunteers took part in the study and donated their hair samples. The analytes were extracted and then separated using a reversed-phase Poroshell EC-C18 column, eluted using two mobile phases, and quantified using LC/MS-MS system. The method was validated in human hair using parameters such as linearity, intra- and inter-day accuracy, and precision and recovery. The method was then used to detect vitamin B3 and B6 vitamers in the human hair samples. Of all the vitamin B3 and B6 vitamers tested, only nicotinamide was detected and quantified in human hair. Of the 40 samples analysed, 12 were in the range 100–200 pg/mg, 15 in the range 200–500 pg/mg, 9 in the range of 500–4000 pg/mg. The LC/MS-MS method is effective, sensitive, and robust for the detection of vitamin B3 and its vitamer nicotinamide in human hair samples. This developed hair test can be used in clinical examination to complement blood and urine tests for the long-term deficiency, detection, and quantification of nicotinamide.

show abstract

A Non-Invasive Hair Test to Determine Vitamin D3 Levels

Cited by 5 publications

References 31 publications

A Novel Biosensor and Algorithm to Predict Vitamin D Status by Measuring Skin Impedance

A Novel Biosensor and Algorithm to Predict Vitamin D Status by Measuring Skin Impedance

Sample preparation techniques for extraction of vitamin D metabolites from non-conventional biological sample matrices prior to LC–MS/MS analysis

Determination of Vitamin B3 Vitamer (Nicotinamide) and Vitamin B6 Vitamers in Human Hair Using LC-MS/MS

Contact Info

Product

Resources

About