A non-pathogenic and optically high concentrated (R,R)-2,3-butanediol biosynthesizing Klebsiella strain

Lee, Soojin; Kim, Borim; Yang, Jeongmo; Jeong, Daun; Park, Soo-Hyun; Lee, Jinwon

doi:10.1016/j.jbiotec.2015.06.385

Cited by 12 publications

(3 citation statements)

References 26 publications

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“…To increase the final 2,3-butanediol concentration, additional glucose supplementation during the cultivation is essential [10, 15]. Additionally, genetic engineering to decrease by-product formation and to enable production of optically pure 2,3-butanediol will be beneficial [12, 51, 52]. Furthermore, the cultivation medium has to be optimized with respect to utilization of cheaper carbon sources and media components [18, 23, 30, 53, 54].…”

Section: Discussionmentioning

confidence: 99%

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

et al. 2019

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Background Production of 2,3-butanediol from renewable resources is a promising measure to decrease the consumption of fossil resources in the chemical industry. One of the most influential parameters on biotechnological 2,3-butanediol production is the oxygen availability during the cultivation. As 2,3-butanediol is produced under microaerobic process conditions, a well-controlled oxygen supply is the key parameter to control biomass formation and 2,3-butanediol production. As biomass is on the one hand not the final product, but on the other hand the essential biocatalyst, the optimal compromise between biomass formation and 2,3-butanediol production has to be defined. Results A shake flask methodology is presented to evaluate the effects of oxygen availability on 2,3-butanediol production with Bacillus licheniformis DSM 8785 by variation of the filling volume. A defined two-stage cultivation strategy was developed to investigate the metabolic response to different defined maximum oxygen transfer capacities at equal initial growth conditions. The respiratory quotient was measured online to determine the point of glucose depletion, as 2,3-butanediol is consumed afterwards. Based on this strategy, comparable results to stirred tank reactors were achieved. The highest space–time yield (1.3 g/L/h) and a 2,3-butanediol concentration of 68 g/L combined with low acetoin concentrations and avoided glycerol formation were achieved at a maximum oxygen transfer capacity of 13 mmol/L/h. The highest overall 2,3-butanediol concentration of 78 g/L was observed at a maximum oxygen transfer capacity of 4 mmol/L/h. Conclusions The presented shake flask approach reduces the experimental effort and costs providing a fast and reliable methodology to investigate the effects of oxygen availability. This can be applied especially on product and by-product formation under microaerobic conditions. Utilization of the maximum oxygen transfer capacity as measure for the oxygen availability allows for an easy adaption to other bioreactor setups and scales. Electronic supplementary material The online version of this article (10.1186/s12934-019-1126-9) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

et al. 2019

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“…The NADH availability and the carbon flux towards BDO production were enhanced leading to BDO conversion yield of 0.46 g g -1 . Lee et al (2015) developed a mutant K. pneumoniae strain by deleting the protein secreting outer core polysaccharides. Further, overexpression of the glycerol dehydrogenase gene (gldA and dhaD) and deletion of ldhA enhanced the production of D-BDO to 61 g L -1 in a fed-batch cultivation strategy using glycerol as carbon source.…”

Section: Klebsiella Spmentioning

confidence: 99%

Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances

Maina

Prabhu

Narisetty

et al. 2022

Biotechnology Advances

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“…Optically pure (2R,3R)-BDO can be produced by knocking out the endogenous gene encoding (2R,3S)-BDO dehydrogenase and thus preventing (2R,3S)-BDO formation. Using this strategy, various recombinant strains including K. pneumonia, [84] S. marcescens, [85] Enterobacter cloacae, [86] and Bacillus licheniformis [87,88] have been developed for the production of (2R,3R)-BDO. Alternatively, budC was deleted in recombinant K. oxytoca strain with disruption of ldhA and pflB genes by integration of the Paenibacillus polymyxa bdh gene.…”

Section: -Butanolmentioning

confidence: 99%

Recent advances in the microbial production of C4 alcohols by metabolically engineered microorganisms

Yoo

Sohn

Son

et al. 2021

Biotechnology Journal

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Background:The heavy global dependence on petroleum-based industries has led to serious environmental problems, including climate change and global warming. As a result, there have been calls for a paradigm shift towards the use of biorefineries, which employ natural and engineered microorganisms that can utilize various carbon sources from renewable resources as host strains for the carbon-neutral production of target products.Purpose and Scope: C4 alcohols are versatile chemicals that can be used directly as biofuels and bulk chemicals and in the production of value-added materials such as plastics, cosmetics, and pharmaceuticals. C4 alcohols can be effectively produced by microorganisms using DCEO biotechnology (tools to design, construct, evaluate, and optimize) and metabolic engineering strategies. Summary of New Synthesis and Conclusions:In this review, we summarize the production strategies and various synthetic tools available for the production of C4 alcohols and discuss the potential development of microbial cell factories, including the optimization of fermentation processes, that offer cost competitiveness and potential industrial commercialization.

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A non-pathogenic and optically high concentrated (R,R)-2,3-butanediol biosynthesizing Klebsiella strain

Cited by 12 publications

References 26 publications

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances

Recent advances in the microbial production of C4 alcohols by metabolically engineered microorganisms

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