A novel alkaline surfactant-stable keratinase with superior feather-degrading potential based on library screening strategy

Su, Chang; Gong, Jin‐Song; Zhang, Rongxian; Tao, Li-Yan; Dou, Wenfang; Zhang, Dandan; Li, Heng; Lü, Zhen-Ming; Xu, Zhenghong; Shi, Jin‐Song

doi:10.1016/j.ijbiomac.2016.11.045

Cited by 44 publications

(27 citation statements)

References 31 publications

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“…Moreover, the detergent SDS enhanced the enzyme activity by 2.79 folds, thereby suggesting that KerP could resist the detergent and that the detergent could increase the surface contact between the feathers and the enzyme. Compared to other keratinases (Su et al 2017a;Zhang et al 2016), KerP has higher potential for application in commercial cleaning or bath products that contain SDS.…”

Section: Effects Of Chemical Compounds On Kerp Activitymentioning

confidence: 99%

Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis

Dong

Chang

Chen

2017

Bioresour. Bioprocess.

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Background: Keratins, insoluble proteins with a robust structure, are a major component of epidermal tissue and appendages such as hair, feathers, nails, and walls. Keratinous waste mainly emanates from poultry and leather industries, thereby severely contaminating the environment. Keratinase can lyse proteins with robust cross-linked structures, such as keratin, and can hence be used in animal feed, fertilizer, detergent, leather, pharmaceutical, and cosmetic industries. Bacillus polyfermenticus B4, isolated from feather compost, secretes keratinase to metabolize feathers. Hence, this study aimed to investigate the enzymatic characteristics and recombinant production of keratinase from B. polyfermenticus B4.Methods: A novel keratinase KerP was isolated from B. polyfermenticus B4 and overexpressed in B. subtilis PT5, via the T7 promoter. Results:The highest keratinolytic activity of recombinant KerP was observed at pH 9.0 and 60 °C. Enzyme activity was enhanced with Fe

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Section: Effects Of Chemical Compounds On Kerp Activitymentioning

confidence: 99%

Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis

Dong

Chang

Chen

2017

Bioresour. Bioprocess.

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“…A novel recombinant keratinase expressed in E. coli BL21 (DE3) exhibited high specificity towards some substrates including wool. The protease has the potential for application in wool processing (Su et al 2017). Several strains of bacteria like Bacillus, Exiguobacterium, Deinococcus and Micrococcus isolated from Patagonian Merino wool, were reported to produce wool-degrading enzymes.…”

Section: Proteases For Biopolishing Of Woolmentioning

confidence: 99%

Potential application spectrum of microbial proteases for clean and green industrial production

Singh

Bajaj

2017

Energ. Ecol. Environ.

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Enzymes are the cornerstones of metabolism and constitute the fundamental basis for existence of life. However, recently enzymes are being implicated in diverse industrial processes because of their specific and fast action for efficient bioconversion of substrate to product, and their capability to save raw materials, energy and chemicals for various manufacturing processes. Enzymes are considered as environment-friendly (green) chemicals that may potentially help replacing completely or reducing the usage of hazardous chemicals for industrial processes, thus promising sustainable production and manufacturing. Among various industrial enzymes microbial proteases dominate the world enzyme market due to their multifaceted application potential in varied bioindustries like food, pharmaceutical, textile, photographic, leather and detergent. Promising applications of proteases in agricultural sector for instance may include biocontrol of pests, degumming of silk, selective delignification of hemp and wool processing. However, for successful industrial applications the proteases must be robust enough to suit the process conditions which are generally hostile. Proteases intended for industrial applications must have activity and stability over wide range of temperature and pH extremes for prolonged time periods and even in the presence of various potential enzyme inhibitors. Of various microbial proteases those from Bacillus spp. have got special significance because the latter are known for their ability to produce sturdy enzymes that might have suitability for industrial process conditions. The current article presents an interpretive summary of the recent developments on application potential of proteases for various industries.

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“…Keratinases (EC 3.4.21/24/99.11) are a class of proteases that hydrolyze keratin, an advantage over other proteases (Brandelli, Daroit and Riffel 2010;Gegeckas et al 2018). These enzymes have applications such as biotechnology (Okoroma et al 2012;Mazotto et al 2013; Paul et al 2014; Brandelli, Sala and Kalil 2015) and waste degradation (Fang et al 2013a;Yusuf et al 2016;Su et al 2017;Abdel-Fattah et al 2018;Thankaswamy et al 2018).…”

Section: Introduction Highlightsmentioning

confidence: 99%

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

Czapela

Kubeneck

Preczeski

et al. 2020

Preprint

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Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

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A novel alkaline surfactant-stable keratinase with superior feather-degrading potential based on library screening strategy

Cited by 44 publications

References 31 publications

Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis

Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis

Potential application spectrum of microbial proteases for clean and green industrial production

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

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