A novel animal model of underactive bladder: Analysis of lower urinary tract function in a rat lumbar canal stenosis model

Sekido, Noritoshi; Jyoraku, Akira; Okada, Hiroki; Wakamatsu, Daisuke; Matsuya, Hidekazu; Nishiyama, Hiroyuki

doi:10.1002/nau.21255

Cited by 35 publications

(33 citation statements)

References 32 publications

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“…An aliquot of urine was collected before each experiment and examined microscopically for leukocytes in order to rule out significant urinary tract infection (UTI). As shown in the previous study [7], UTI was not identified on urinalysis at CMG, and histological studies of the bladder specimens showed no obvious qualitative difference between sham and LCS rats. Also, there was no significant renal impairment on blood chemistry at the time of CMG.…”

Section: Methodssupporting

confidence: 70%

“…This study further explored awake cystometric findings from a previous study [7]. The procedures of model preparation and the awake cystometric study are briefly described as below.…”

Section: Methodsmentioning

confidence: 99%

“…This model was created by insertion of a small piece of silicone rubber into the L5 to L6 epidural space to compress the cauda equina, and it reveals features of infrasacral neuropathic bladder (NB), namely, impairment of voiding efficiency and a decrease in detrusor contractility. Unexpectedly, maximum cystometric capacity (MCC, mL) in some LCS rats became smaller than that in sham rats, which is why MCC in LCS rats (1.58 ± 0.68 mL, mean ± SEM) was not significantly different from that in sham rats (1.34 ± 0.23 mL) [7]. In clinical settings, it was reported that DO was noted in 29% of LCS patients [9].…”

Section: Introductionmentioning

confidence: 93%

“…Recently, we reported a rat model of lumbar canal stenosis (LCS) as a UAB model [7] [8]. This model was created by insertion of a small piece of silicone rubber into the L5 to L6 epidural space to compress the cauda equina, and it reveals features of infrasacral neuropathic bladder (NB), namely, impairment of voiding efficiency and a decrease in detrusor contractility.…”

Section: Introductionmentioning

confidence: 99%

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Does an “Overactive to Underactive Bladder Transition” Phenomenon Exist in a Rat Lumbar Spinal Canal Stenosis Model?

Sekido¹,

Kida²,

Wakamatsu³

et al. 2015

OJU

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Purpose: To investigate the association between the maximum cystometric capacity (MCC) and other cystometric parameters in lumbar canal stenosis (LCS) rats. Material and Methods: One small hole was drilled at the fifth lumbar vertebral arch (Sham), and a rectangular piece of silicone rubber was then placed in the epidural space (LCS) of Wister rats. Two weeks after surgery, awake cystometry was performed. LCS rats were divided into three groups: Group A (n = 5, MCC < 0.87 mL), Group B (n = 13, MCC 0.87 -1.81 mL), and Group C (n = 14, MCC > 1.81 mL). Cystometric parameters were investigated in sham and LCS groups. Results: MCC did not significantly correlate to the frequency of non-voiding contractions (NVCs), voided volume (VV), or maximum intravesical pressure during voiding (Pmax), but significantly positively correlated to postvoid residual urine volume (PVR) and residual urine rate (RUR) (Spearman's correlation coefficients (ρ) = 0.8973 (p < 0.0001) and 0.4915 (p = 0.0068), respectively). Compared with the sham rats, LCS rats in each group revealed significantly smaller VV, larger RUR, and lower Pmax. On the other hand, among LCS rats, VV, RUR, and Pmax were not significantly different. The frequency of NVCs in each LCS group was not significantly different from that in sham-operated rats (Tukey-Kramer's HSD test). However, a Jonckheere-Terpstra trend test revealed a significant trend toward higher NVCs in the order of sham, Groups C, B, and A (p = 0.036). Conclusions: LCS rats showed the same degree of detrusor underactivity regardless of MCC. NVCs did not significantly increase in LCS rats with decreased MCC, but the trend toward higher NVCs with smaller MCC was significant.

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Section: Methodssupporting

confidence: 70%

“…This study further explored awake cystometric findings from a previous study [7]. The procedures of model preparation and the awake cystometric study are briefly described as below.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Does an “Overactive to Underactive Bladder Transition” Phenomenon Exist in a Rat Lumbar Spinal Canal Stenosis Model?

Sekido¹,

Kida²,

Wakamatsu³

et al. 2015

OJU

Self Cite

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“…At the evaluated time points of this study, the effects caused by sucrose ingestion on bladder function may be associated with increased drinking volumes leading to larger bladders, without activating oxidative stress pathways, a situation observed in rats with lumbar canal stenosis or diabetes [16,17]. Accordingly, the detrusor hypertrophy detected during sucrose feeding [14], but not oxidative cell or nerve damage, strongly supports the hypothesis of altered regulation of transmitter release sources leading to underactive bladder dysfunction [12].…”

Section: Discussionsupporting

confidence: 71%

Enhanced production of nitric oxide may contribute to the induction of detrusor underactivity in sucrose fed rats

Boone

Muñoz

2015

Bladder

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OBJECTIVE:In a previous study we showed that sucrose feeding creates cystometric properties resembling detrusor underactivity (DU) associated with enhanced production of nitric oxide (NO) in rats. Our present objective was to evaluate the possible role of P2X-purinergic and cholinergic receptors as molecular mechanisms underlying DU-like conditions associated with enhanced production of NO and altered contractile properties in rat bladder strips. MATERIALS AND METHODS:Female Sprague-Dawley rats had ad-libitum access to 5% sucrose in water for two to four weeks. Isometric detrusor contractions were characterized in response to electrical field stimulation, the cholinergic agonist carbachol, the P2X agonist alpha, beta-methylene-ATP or KCl-induced depolarization. ATP and NO release rates were determined from bladder strips after stimulation of cholinergic or P2X-type purinergic receptors. RESULTS:Electrical field stimulations generated stronger contractile responses in the strips from sucrose fed animals, while KCl induced similar contractions. The release of ATP after cholinergic or purinergic stimulation was similar in control or sucrose fed bladder strips. Nonetheless, NO production was significantly higher when pharmacologically triggered with either cholinergic or purinergic stimulation in the bladder strips from rats drinking sucrose for four weeks. The contractile responses to carbachol or abMe-ATP were also significantly reduced in this group. CONCLUSIONS:Detrusor contractions in these underactive-like conditions are gradual, and may be generated by excessive NO production that is mediated by individual activation of cholinergic or purinergic receptors, with higher effects by the latest. Our results suggest that nitric oxide production and not impaired contractile mechanisms are involved in this rat model of detrusor underactivity.

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Detrusor underactivity: Pathophysiological considerations, models and proposals for future research. ICI-RS 2013

Koeveringe

Rademakers

Birder

et al. 2014

Neurourol. Urodynam.

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Aims Detrusor underactivity, resulting in either prolonged or inefficient voiding, is a common clinical problem for which treatment options are currently limited. The aim of this report is to summarize current understanding of the clinical observation and its underlying pathophysiological entities. Methods This report results from presentations and subsequent discussion at the International Consultation on Incontinence Research Society (ICI-RS) in Bristol, 2013. Results and Conclusions The recommendations made by the ICI-RS panel include: Development of study tools based on a system’s pathophysiological approach, correlation of in vitro and in vivo data in experimental animals and humans, and development of more comprehensive translational animal models. In addition, there is a need for longitudinal patient data to define risk groups and for the development of screening tools. In the near-future these recommendations should lead to a better understanding of detrusor underactivity and its pathophysiological background.

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A novel animal model of underactive bladder: Analysis of lower urinary tract function in a rat lumbar canal stenosis model

Abstract: This rat model requires a relatively simple surgical procedure and has characteristics of neurogenic UAB. It seems to be useful in the pathophysiological elucidation of UAB and might have potential for assessment of pharmacotherapy of UAB.

Cited by 35 publications

References 32 publications

Does an “Overactive to Underactive Bladder Transition” Phenomenon Exist in a Rat Lumbar Spinal Canal Stenosis Model?

Does an “Overactive to Underactive Bladder Transition” Phenomenon Exist in a Rat Lumbar Spinal Canal Stenosis Model?

Enhanced production of nitric oxide may contribute to the induction of detrusor underactivity in sucrose fed rats

Detrusor underactivity: Pathophysiological considerations, models and proposals for future research. ICI-RS 2013

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