A novel application of Onyx™ monolithic column for simultaneous determination of salicylic acid and triamcinolone acetonide by sequential injection chromatography

Improvements in the Separation Capabilities of Sequential Injection Chromatography: Determination of Intracellular Dissolved Free Amino Acid Profiles in Three Taxonomic Groups of Microalgae

“…Although the use of 50 mm monolithic columns in SIC or multisyringe approaches has been reported previously (Chocholous et al, 2007b;Guzmán-Mar et al, 2011) the present paper is the first to report methanol concentrations as high as 65% (v/v). As methanol generates more pressure than acetonitrile in liquid chromatography, the absence of leakage under the conditions used proves the adequacy of the mechanical configurations made in the system.…”

“…The new configuration of the SIC system allowed the use of a 50 mm long monolithic column (plus a 5 mm guard column) without problems of leakage of mobile phases through the selection or relief valves at a flow rate of 30 μL/s (1.8 mL/min). Although the use of 50 mm monolithic columns in SIC or multi‐syringe approaches has been reported previously (Chocholous et al ., ; Guzmán‐Mar et al ., ) the present paper is the first to report methanol concentrations as high as 65% (v/v). As methanol generates more pressure than acetonitrile in liquid chromatography, the absence of leakage under the conditions used proves the adequacy of the mechanical configurations made in the system.…”

Application of a lanthanide composite nanoparticle‐sensitized luminescence method for the determination of salicylic acid in pharmaceutical formulations and human plasma

“…the solid-phase spectrophotometric (SPS) technique (10), the flow-injection analysis-atomic absorption spectroscopy (FIA-AAS) method (11), CZE (12), solid-phase extraction (SPE)-reversed phase (RP) HPLC-electrospray ionization mass spectrometry (ESI-MS)/fluorescence detection (FLD)/DAD (13), and sequential injection chromatography (SIC) (14) have been reported for the determination of SA, as well as for the simultaneous determination of this chemical with other anti-inflammatory agents. Apart from the methods mentioned, new methods have also been put forward in recent articles.…”

Automated Quality Control of Pharmaceuticals using Sequential Injection Chromatography (SIC)

“…Some representative pharmaceutical applications of SIC can be found in Table 1 [7,[12][13][14][15][16][17][18][19][20][21][22]. Looking at the methods and the analytical conditions included in Table 1 it is evident that most of the so far reported and published protocols involve separation of simple two/ three component mixtures employing isocratic elution and universal UV detection.…”

“…Very recently Chocholous et al [19] reported, for the first time, an interesting alternative that could expand the potentials of SIC [26]. This alternative was the incorporation of short HPLC columns based on the novel "fused core" or "core shell" technology.…”