A novel approach to ion separations in solution: synchronized cyclic capillary electrophoresis (SCCE)

Microchannel electrophoretic separation of biogenic amines by micellar electrokinetic chromatography

“…Electrophoresis 1999, 20, 118±126 Microchannel MEKC of biogenic amines 123 consequence, decrease of the effective injected sample plug size [4,8]. Further improvements in efficiency could be achievable by using narrower injection channels and smaller detector view points.…”

“…Using electric fields to direct and control fluid flow eliminates the need for micromoving parts such as pumps and valves, allowing for a convenient integration process of complete assays. Over the past six years, applications of microfabricated capillary electrophoresis chips have been successfully demonstrated in the fields of free zone electrophoresis [4±6], synchronized cyclic electrophoresis [7,8], gel electrophoresis [9±12], openchannel electrochromatography [13] and micellar electrokinetic chromatography (MEKC) [14±16]. Monolithic devices that integrate sample processing, chemical reactions and analysis included free zone electrophoresis with precolumn [17] or postcolumn [18,19] reactors, devices which allow solvent mixing for isocratic and gradient elution MEKC [16], DNA restriction digestions with fragment sizing [20], PCR amplification and electrophoretic analysis [21], cell lysis, PCR amplification and electrophoretic analysis [22].…”

Cyclic electrophoretic and chromatographic separation methods

“…The feasibility of SCCE was first demonstrated by Burggraf et al [13,16] who made good use of the ease of sample manipulation on a planar glass chip device. The authors used a square channel geometry; Fig.…”

Integrated chip‐based capillary electrophoresis

“…A novel electrophoretic separation concept, dubbed 'synchronized cyclic capillary electrophoresis' (SCCE), is based on repeated column switching [40, [52][53][54]. The principle of SCCE is shown in Fig.…”