A Novel ATP-Binding Cassette Transporter Involved in Multidrug Resistance in the Phytopathogenic Fungus
            <i>Penicillium digitatum</i>

Nakaune, Ryoji; Adachi, Kenjiro; Nawata, Osamu; Tomiyama, Masamitsu; Akutsu, Katsumi; Hibi, Tadaaki

doi:10.1128/aem.64.10.3983-3988.1998

Cited by 182 publications

(90 citation statements)

References 41 publications

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“…there is often incomplete cross-resistance among azoles) (Cools et al 2013). Currently, three main mechanisms of azole resistance have been identified in plant pathogenic fungi: (i) alteration of the CYP51 enzyme (D elye et al 1998;Wyand & Brown 2005;Leroux & Walker 2011), (ii) overexpression of CYP51 (Schnabel & Jones 2001;Sun et al 2011;Cools et al 2012) and (iii) activation of efflux pump activity to reduce the intracellular accumulation of azole compounds (Nakaune et al 1998(Nakaune et al , 2002Hayashi et al 2002;Omrane et al 2015). These mechanisms can either act alone or in combination to produce various levels of azole sensitivity (Cools et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Multilocus resistance evolution to azole fungicides in fungal plant pathogen populations

2016

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Evolution of fungicide resistance is a major threat to food production in agricultural ecosystems. Fungal pathogens rapidly evolved resistance to all classes of fungicides applied to the field. Resistance to the commonly used azole fungicides is thought to be driven mainly by mutations in a gene (CYP51) encoding a protein of the ergosterol biosynthesis pathway. However, some fungi gained azole resistance independently of CYP51 mutations and the mechanisms leading to CYP51-independent resistance are poorly understood. We used whole-genome sequencing and genome-wide association studies (GWAS) to perform an unbiased screen of azole resistance loci in Rhynchosporium commune, the causal agent of the barley scald disease. We assayed cyproconazole resistance in 120 isolates collected from nine populations worldwide. We found that mutations in highly conserved genes encoding the vacuolar cation channel YVC1, a transcription activator, and a saccharopine dehydrogenase made significant contributions to fungicide resistance. These three genes were not previously known to confer resistance in plant pathogens. However, YVC1 is involved in a conserved stress response pathway known to respond to azoles in human pathogenic fungi. We also performed GWAS to identify genetic polymorphism linked to fungal growth rates. We found that loci conferring increased fungicide resistance were negatively impacting growth rates, suggesting that fungicide resistance evolution imposed costs. Analyses of population structure showed that resistance mutations were likely introduced into local populations through gene flow. Multilocus resistance evolution to fungicides shows how pathogen populations can evolve a complex genetic architecture for an important phenotypic trait within a short time span.

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Section: Introductionmentioning

confidence: 99%

Multilocus resistance evolution to azole fungicides in fungal plant pathogen populations

2016

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“…A cDNA macroarray approach (Fig. 5) was employed to test the hypothesis that the lower sensitivities of some P. infestans strains was due to enhanced ABC transporter expression, as in many non-oomycetes (Hayashi et al, 2001;Laing et al, 1998;Lyons and White, 2000;Nakaune et al, 1998;Slaven et al, 2002). Polymerase chain reaction (PCR)-amplified fragments of the 54 ABC transporter genes plus actin (ActA) were spotted on membranes, which were hybridized with 32 P-cDNA from four 1114 × 510 mex/mex offspring.…”

Section: Transcription Of Abc Transporter Genes In P Infestansmentioning

confidence: 99%

“…It is reasonable to propose that ABC transporters are involved owing to similarities with the phenomenon of multidrug resistance. This often results from increased transcription of ABC transporters as a consequence of promoter mutations or gene amplification (Hayashi et al, 2001;Laing et al, 1998;Lyons and White, 2000;Nakaune et al, 1998;Slaven et al, 2002). In addition, over-expressing ABC transporters in transgenic Magnaporthe grisea and Aspergillus nidulans decreased their sensitivities to diverse toxicants (Andrade et al, 2000;Lee et al, 2005).…”

Section: Figmentioning

confidence: 99%

“…Reductions in sensitivity mediated by ABC transporters in fungal pathogens of humans has also been described (Ben-Yaacov et al ., 1994). In plant pathogenic fungi, laboratory studies have demonstrated that ABC transporters have roles in virulence or in toxicant efflux, but their relevance to fungicide insensitivity in the field is less well understood (Hayashi et al ., 2001;Lee et al ., 2005;Nakaune et al ., 1998;Urban et al ., 1999). That factors besides 'major genes' influence the sensitivity of plant pathogens to crop protection chemicals is evident from surveys of field isolates of various ascomycetes, basidiomycetes and oomycetes (Franke et al ., 1998;Gisi et al ., 1997;Olaya and Koller, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Judelson¹,

Senthil²

2005

Molecular Plant Pathology

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SUMMARY Isolates of the oomycete Phytophthora infestans exhibit a wide range of intrinsic sensitivities to fungicides, which potentially influences the application rates of chemicals needed to control potato late blight. To help understand what determines such levels of sensitivity, a genetic approach was employed which followed the segregation of sensitivities to structurally diverse fungicides such as metalaxyl and trifloxystrobin. Progeny exhibited broad distributions of sensitivity phenotypes, consistent with the behaviour of a quantitative trait. Measurements of the inhibition of strains by seven fungicides revealed that basal sensitivities to metalaxyl and trifloxystrobin, and to cymoxanil and dimethomorph, correlated at the 95% confidence level. These compounds have distinct modes of action, suggesting the involvement of a multifungicide efflux phenomenon mediated by ABC transporters. To determine whether such proteins contribute to variation in sensitivity, 41 full transporters and 13 half transporters were identified from P. infestans and their mRNA levels compared in strains exhibiting higher or lower sensitivities to fungicides. No correlation was observed between the expression of any ABC transporter and fungicide sensitivity. Other genes, or variation in the activities of the transporters, may therefore explain the differences between strains. Five ABC transporters were induced by several fungicides in strains with both higher and lower sensitivities to fungicides, which probably reflects the existence of a network for protecting against natural and artificial toxins.

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“…The PDR5 is a classical multidrug transporter and can confer resistance to many unrelated toxicants (Balzi et al, 1994). Since then, the ABC transporters have been cloned from several plant pathogenic fungi, including Botrytis cinerea (Vermeulen et al, 2001), Gibberella pulicaris (Fleibner et al, 2002), Magnaporthe grisea (Lee et al, 2005), Monilinia fructicola (Schnabel et al, 2003), Mycosphaerella graminicola (Stergiopoulos et al, 2002) and Penicillium digitatum (Nakaune et al, 1998). However, no ABC transporters have been characterized from a biotrophic pathogenic fungus.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression Analysis of a Putative ABC Transporter Gene BgABC1 from the Biotrophic Pathogenic Fungus Blumeria graminis f. sp. tritici

Hu¹,

Yan²,

Ma³

2007

Journal of Phytopathology

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A putative ATP-binding cassette (ABC) transporter gene (BgABC1) was isolated from the biotrophic pathogenic fungus Blumeria graminis f. sp. tritici (Bgt). Analysis of the deduced amino acid sequence of BgABC1 showed that the BgABC1 protein had a conserved nucleotide-binding fold in the N-terminus, and six transmembrane domains (TMDs) in the C-terminus. Analysis of the BgABC1 expression using realtime polymerase chain reaction showed that expression of the gene was increased significantly when the fungus was growing in wheat seedlings treated with 14a-demethylase-inhibiting fungicide, triadimefon. The results indicated that the BgABC1 was involved in the protection of the fungus against fungicide toxicity.

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A Novel ATP-Binding Cassette Transporter Involved in Multidrug Resistance in the Phytopathogenic Fungus Penicillium digitatum

Cited by 182 publications

References 41 publications

Multilocus resistance evolution to azole fungicides in fungal plant pathogen populations

Multilocus resistance evolution to azole fungicides in fungal plant pathogen populations

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Cloning and Expression Analysis of a Putative ABC Transporter Gene BgABC1 from the Biotrophic Pathogenic Fungus Blumeria graminis f. sp. tritici

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