A Novel Cell Penetrating Peptide for the Differentiation of Human Neural Stem Cells

Ma, Weili; Jin, Geun‐woo; Gehret, Paul M; Chada, Neil C; Suh, Won Hyuck

doi:10.3390/biom8030048

Cited by 11 publications

(5 citation statements)

References 89 publications

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“…CPP can also deliver nucleic acid such as DNA, mRNA and siRNA (Meade et al, 2007;Srimanee et al, 2018;Yokoo et al, 2021;Mahjoubin-Tehran et al, 2022) and can mediate anti-cancer effects (Klimpel et al, 2019). CPP can promote neural stem cell differentiation (Ma et al, 2018) and CPP-conjugated with reprogramming factors increased the rate of induced pluripotent stem cell formation (Lim et al, 2014). CPP-conjugated morpholino oligos has been used in vivo broadly (Moulton et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Cellular internalization effect of Ara27 in various cell lines

Kim

Park

Seo

et al. 2022

J Anim Reprod Biotechnol

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Protein and peptide candidates are screened to apply therapeutic application as a drug. Ensuring that these candidates are delivered and maximized effectiveness is still challenging and a variety of studies are ongoing. As drug delivery system vehicles, cell-penetrating peptide (CPP) can deliver various kinds of cargo into the cell cytosol. In a previous study, we developed Ara27 CPP, which are a zinc knuckle family protein of Arabidopsis, and confirmed internalization in human dermal fibroblasts and human dental pulp stem cells at low concentration with short time treatment condition without any toxicity. Ara27, an amphipathic CPP, could be modified and utilized in the biomedical field excluding the risk of toxicity. Therefore, we would like to confirm the non-toxic induced penetrating ability of Ara27 in various cell lines. The purpose of this study was to screen the cell internalization ability of Ara27 in various cell lines and to confirm Ara27 as a promising core CPP structure. First, Ara27 was screened to confirm non-toxicity concentration. Then, fluorescence-labeled Ara27 was treated on human normal cell lines, cancer cell lines and animal cell lines to identify the cellular internalization of Ara27. Ara27 was well intracellular localized in all cell lines and the intensity of fluorescence was remarkably increased in time pass manner. These results indicate that Ara27 has the potential as a core structure for applications in various drug delivery systems.

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Section: Discussionmentioning

confidence: 99%

Cellular internalization effect of Ara27 in various cell lines

Kim

Park

Seo

et al. 2022

J Anim Reprod Biotechnol

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“…The peptides AEDG and AEDP invoke differentiation of pluripotent cells of the epidermis, mesenchyme, and nervous tissue [17,20,34]. Peptides KE, AED, KED, AEDG [34], and AAAAEKAAAAEKAAAAEK [40] activate neuronal differentiation. It has been demonstrated that the AEDL peptide induces the differentiation of lung cells by regulating the expression of the NKX2-1, SCGB1A1, SCGB3A2, FOXA1, and FOXA2 genes [41].…”

Section: Peptide Regulation Of Cell Differentiationmentioning

confidence: 99%

Peptide Regulation of Gene Expression: A Systematic Review

et al. 2021

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Peptides are characterized by their wide range of biological activity: they regulate functions of the endocrine, nervous, and immune systems. The mechanism of such action of peptides involves their ability to regulate gene expression and protein synthesis in plants, microorganisms, insects, birds, rodents, primates, and humans. Short peptides, consisting of 2–7 amino acid residues, can penetrate into the nuclei and nucleoli of cells and interact with the nucleosome, the histone proteins, and both single- and double-stranded DNA. DNA–peptide interactions, including sequence recognition in gene promoters, are important for template-directed synthetic reactions, replication, transcription, and reparation. Peptides can regulate the status of DNA methylation, which is an epigenetic mechanism for the activation or repression of genes in both the normal condition, as well as in cases of pathology and senescence. In this context, one can assume that short peptides were evolutionarily among the first signaling molecules that regulated the reactions of template-directed syntheses. This situation enhances the prospects of developing effective and safe immunoregulatory, neuroprotective, antimicrobial, antiviral, and other drugs based on short peptides.

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“…CPPs can overcome some of the limitations of viral vectors and liposome-based delivery systems. The properties of CPPs not only allow for effective cellular internalization but also offer potential solutions to challenges encountered with viral vector-based gene delivery systems [19].…”

Section: Introductionmentioning

confidence: 99%

Peptosome: A New Efficient Transfection Tool as an Alternative to Liposome

Manteghi,

Can,

Kocagoz

2024

IJMS

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Gene therapy is one of the most promising techniques for treating genetic diseases and cancer. The current most important problem in gene therapy is gene delivery. Viral and non-viral vectors like liposomes, used for gene delivery, have many limitations. We have developed new hybrid peptides by combining cell-penetrating peptides (CPPs) with the DNA-binding domain of the human histone H4 protein. These small peptides bind to DNA molecules through their histone domain, leaving the CPP part free and available for binding and penetration into cells, forming complexes that we named “peptosomes”. We evaluated the transfection efficiency of several hybrid peptides by delivering a plasmid carrying the green fluorescent protein gene and following its expression by fluorescent microscopy. Among several hybrid peptides, TM3 achieved a gene delivery efficiency of 76%, compared to 52% for Lipofectamine 2000. TM3 peptosomes may become important gene delivery tools with several advantages over current gene delivery agents.

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A Novel Cell Penetrating Peptide for the Differentiation of Human Neural Stem Cells

Cited by 11 publications

References 89 publications

Cellular internalization effect of Ara27 in various cell lines

Cellular internalization effect of Ara27 in various cell lines

Peptide Regulation of Gene Expression: A Systematic Review

Peptosome: A New Efficient Transfection Tool as an Alternative to Liposome

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