A novel co-culture process with Zymomonas mobilis and Pichia stipitis for efficient ethanol production on glucose/xylose mixtures

Fu, Nan; Peiris, Paul; Markham, Julie L.; Bavor, John

doi:10.1016/j.enzmictec.2009.04.006

Cited by 138 publications

(108 citation statements)

References 26 publications

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“…After these times the ethanol concentration was notably reduced, probably due to its consumption by the yeast as a consequence of the fast exhaustion of sugars from the media. Similar behaviour has already been observed for other yeast strains (Fu et al, 2009;Groleau et al, 1995). The differences on ethanol production and sugars consumption observed in the experiments were statistically analysed, aiming to verify the influence of each variable on ethanol formation by S. stipitis.…”

Section: Hydrolysate Supplementation With Nutrientsmentioning

confidence: 82%

Ethanol production by a new pentose‐fermenting yeast strain, Scheffersomyces stipitis UFMG‐IMH 43.2, isolated from the Brazilian forest

Ferreira

Mussatto

Cadete

et al. 2011

Yeast

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The ability of a recently isolated Scheffersomyces stipitis strain (UFMG-IMH 43.2) to produce ethanol from xylose was evaluated. For the assays, a hemicellulosic hydrolysate produced by dilute acid hydrolysis of sugarcane bagasse was used as the fermentation medium. Initially, the necessity of adding nutrients (MgSO 4 ·7H 2 O, yeast extract and/or urea) to this medium was verified, and the yeast extract

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Section: Hydrolysate Supplementation With Nutrientsmentioning

confidence: 82%

Ethanol production by a new pentose‐fermenting yeast strain, Scheffersomyces stipitis UFMG‐IMH 43.2, isolated from the Brazilian forest

Ferreira

Mussatto

Cadete

et al. 2011

Yeast

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“…Nonetheless, glucose and fructose are the mayor carbohydrates presented in citrus waste [10,11], a complete and efficient conversion of hexoses and pentoses carbohydrates presented in the lignocellulosic hydrolysates is a prerequisite for maximizing the profitability of an industrial process for bioethanol production [9]. Cell growth obtained by dry weight of the strains C.…”

Section: Capability Of Candidas To Ferment Mixtures Of Carbohydrates mentioning

confidence: 99%

“…Co-culture fermentations may result in increased yield, improved control of product qualities, possibility of utilizing cheaper substrates and potential of improving existing processes [8]. These methods have been described to improve the efficiency of lignocellulosic biomass fermentation, having a great impact on the development of biofuels, bioenergy and biobased products [4,8,9]. On the other hand, carbohydrates concentrations of agroindustrial residues vary depending on the crop used and cultivation conditions.…”

Section: Introductionmentioning

confidence: 99%

“…have shown the ability to ferment pentoses and hexoses carbohydrates from degradation of hemicellulose and cellulose, individually and in co-culture [5][6][7]. The co-culture system appears to be an advantageous system over individual cultures because of the potential for synergistic utilization of the metabolic pathways of the strains involved [8,9]. Co-culture fermentations may result in increased yield, improved control of product qualities, possibility of utilizing cheaper substrates and potential of improving existing processes [8].…”

Section: Introductionmentioning

confidence: 99%

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Capability of Candidas to Ferment Mixtures of Carbohydrates to Alcohol in Free Cells and Co-Culture

Estrada-Martínez¹,

Pacheco-López²,

Ramírez‐Sucre³

et al. 2017

JFSE

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Abstract:Organic biomass is an attractive feedstock for second generation alcohol production. Wild-type strains of the genus Candida showed capabilities different to produce alcohol fermenting a carbohydrates mixture (synthetic medium), individually and in co-culture. Therefore, the main objective of this work was to evaluate the capability of Candida wild-type strains isolated from termite gut and rumen liquid, to ferment the most commonly carbohydrates presented in citrus residues, individually and in co-culture to alcohol production. C. Tropicalis (LR4) presented higher percentage of carbohydrate consumption (74.20% ± 4.60%), alcohol production (44.53 ± 0.01 gL -1 ) and maximal alcohol productivity (6.40 ± 0.01 gL -1 day) than C. Glabrata (T1). Co-culture schemas, CC1 (LR4: 60%; T1: 40%) and CC3 (first LR4 alone and 2 days later T1) presented the highest alcohol production (45.20 ± 1.30 gL -1 and 46.80 ± 2.60 gL -1 , respectively). Maximal alcohol productivity was obtained with CC2 (LR4: 80%; T1: 20%) and CC3 schemas, 7.70 ± 0.29 gL -1 day and 7.80 ± 0.44 gL -1 day, respectively. The results suggest the usefulness of these wild-type strains in co-culture as an alternative to alcohol production from carbohydrates mixtures at concentrations commonly found in citrus waste.

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“…Singh et al (2014) co-cultured Pichia stipitis and Z. mobilis for bioethanol production from kans grass biomass with significant yields and Zhang et al (2016a) employed C. cellulolyticum and hydrogen fermentation bacteria for enhanced biohydrogen production from corn stover with significant differences seen in the metabolites of the co-culture system over the mono-cultures. Other reports of successful bio-catalysis based on microbial consortia have equally been reported (Fu et al, 2009;He et al, 2011;Ho et al, 2011;Li et al, 2011;Quinn et al, 2016;Reddy and Basappa, 1996;Yaun et al, 2016;Zhang et al, 2016b;Zhong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Engineered microbial consortium for the efficient conversion of biomass to biofuels: A preliminary study

Anieto¹

2017

Afr. J. Microbiol. Res.

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This work showed ethanol production by a microbial consortium of Clostridium cellulolyticum and a recombinant Zymomonas mobilis (ZM4 pAA1). The ZM4 pAA1 and wild type ZM4 (ZM4 WT) were first tested on RM medium (ATCC 1341) containing 2% cellobiose as the carbon source. Ethanol production from ZM4 pAA1 was three times higher than that observed from the ZM4 WT. Concomitant with ethanol production was the reduction in OD from 2.00 to 1.580. The ZM4 pAA1 was then co-cultured with C. cellulolyticum using cellobiose and microcrystalline cellulose , respectively, as carbon sources. Results indicate that the ZM4 pAA1 with C. cellulolyticum utilized 2.0 g/L cellobiose, producing as much as 0.40 mM of ethanol, whereas only 0.20 mM ethanol was detected for the ZM4 WT co-cultured with C. cellulolyticum under similar conditions. A consortium of the ZM4 pAA1 and C. cellulolyticum using 7.5 g/L microcrystalline cellulose gave a far lower ethanol yi eld than when using cellobiose. In the latter case, ethanol production was detected within 5 days, whereas it took about 10 days for ethanol to be detectable for the ZM4 WT and C. cellulolyticum. Future efforts will concentrate on identifying suitable partners for the ZM4 pAA1, the correct concentration of feedstocks at which synergy will be observed, as well as optimize medium formulations and inoculation techniques.

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A novel co-culture process with Zymomonas mobilis and Pichia stipitis for efficient ethanol production on glucose/xylose mixtures

Cited by 138 publications

References 26 publications

Ethanol production by a new pentose‐fermenting yeast strain, Scheffersomyces stipitis UFMG‐IMH 43.2, isolated from the Brazilian forest

Ethanol production by a new pentose‐fermenting yeast strain, Scheffersomyces stipitis UFMG‐IMH 43.2, isolated from the Brazilian forest

Capability of Candidas to Ferment Mixtures of Carbohydrates to Alcohol in Free Cells and Co-Culture

Engineered microbial consortium for the efficient conversion of biomass to biofuels: A preliminary study

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