A novel dual-emission fluorescent probe for the simultaneous detection of H₂S and GSH

Abstract

A novel chlorinated coumarin-malononitrile fluorescent probe with three potential reaction sites, which exhibited highly selective, rapid response, low detection limit, and was capable of the simultaneous detection of H2S (λex/em = 515/564 nm) and GSH (λex/em = 430/517 nm), was first proposed. The probe was successfully applied to dual-channel imaging H2S and GSH in MCF-7 cells.

A fluorescent probe for the dual detection of mercury ions and thiols based on a simple coumarin derivative

“…35,36 A novel chlorinated coumarin-malononitrile fluorescent probe with three potential reaction sites was developed, and it demonstrated a highly selective and rapid response to hydrogen sulphide and GSH. 37 It is known that thiophilic Hg(ii) has a strong binding ability with the sulphur atom in biothiols and thus has the potential to disturb the biothiol level in the physiological system. 38 In addition, the reaction between biothiol and Hg(ii) may bring about the inactivation of the enzymes in biothiol.…”

“…For example, certain bifunctional fluorescent probes were capable of sensing Hg( ii ) along with another analyte such as Ag( i ), Cu( ii ), Fe( iii ), Al( iii ), pH change, saccharides or F – . A novel chlorinated coumarin‐malononitrile fluorescent probe with three potential reaction sites was developed, and it demonstrated a highly selective and rapid response to hydrogen sulphide and GSH . It is known that thiophilic Hg( ii ) has a strong binding ability with the sulphur atom in biothiols and thus has the potential to disturb the biothiol level in the physiological system .…”

Salivary Hydrogen Sulfide Measured with a New Highly Sensitive Self-Immolative Coumarin-Based Fluorescent Probe

“…Based on a similar strategy, Zhao and Song demonstrated a series of probes with para-azidobenzyl group attached to the 1,8-naphthalimide [ 36 , 37 ]. Other methods are based on unique dual nucleophilic reactions [ 25 , 38 , 39 ], high binding affinity towards copper ions [ 40 , 41 , 42 ] and a specific addition reaction to unsaturated double bond [ 43 , 44 , 45 ].…”

Fluorescent probes for the simultaneous detection of multiple analytes in biology

“…Using this strategy, probes have been reported with characteristic responses to different transition metals, 63,64 ROS 65 or biothiols. [66][67][68][69][70][71][72] Theoretically, if the probe concentration, kinetics and thermodynamics of the reactions with the targets do not lead to saturation of the probe with one analyte alone, these systems will be capable of reporting on the presence of one or more analytes.…”