A novel extracellular cold-active esterase of Pseudomonas sp. TB11 from glacier No.1: Differential induction, purification and characterisation

Dong, Jun; Zhao, Wei; Gasmalla, Mohammed Abdalbasit A.; Sun, Jing‐Tao; Xiao, Hua; Zhang, Wenbin; Han, Liang; Fan, Yuting; Yan, Feng; Shen, Qiuyun; Yang, Ruijin

doi:10.1016/j.molcatb.2015.07.015

Cited by 8 publications

(1 citation statement)

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“…Active protein was applied to a Superdex 75 10/300 GL gel‐filtration column (1.0 × 30 cm; GE Healthcare, USA). Buffer C (50 mM Tris–HCl, pH 8.5) was used for sample loading and elution, and the flow rate was 0.8 mL/Min . Active fractions were pooled.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a cold‐adapted esterase and mutants from a psychotolerant Pseudomonas sp. strain

Dong

Gasmalla

Zhao

et al. 2017

Biotech and App Biochem

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A cold-adapted esterase-producing strain named T1-39 was isolated from Glacier No. 1, Tianshan, People's Republic of China and identified as Pseudomonas sp. from 16S rRNA sequence analysis. The esterase (EstT1-39) secreted by this strain preferentially hydrolyzed esters of glycerol with short- and medium-chain fatty acids. Mutants of T1-39 were generated by the atmospheric and room temperature plasma method and screened for enhanced esterase activity. Among all the mutants, strain TB11 had 4.45-fold higher esterase productivity than T1-39, with high genetic stability over 10 generations of continuous cultivation. Maximum activity of EstT1-39 and EstTB11 was observed at 30 ℃, pH 9.0 and 25 ℃, pH 8.5, respectively. EstTB11 was thermally more stable (50 ℃ for 1 H) and active over a broader pH range than EstT1-39. EstTB11 also retained 38% of its maximal activity at 0 ℃ and was found to be able to hydrolyze milk fats into short- and medium-chain fatty acids at 4 ℃. The characteristics of EstT1-39 made it a cold-adapted enzyme and the EstTB11 from the mutant, with its higher activity at lower temperatures, may be suitable for the production of aromas and flavors in the dairy industry.

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Section: Methodsmentioning

confidence: 99%